FastQCFastQC Report
Fri 10 Feb 2017
ERR1633364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752874
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT68610.9113078682488703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.656285115437643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27410.3640715445081116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.1742655477543387No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9390.1247220650467409No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8080.10732207514139153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTT352.3872213E-531.71428725
GGTATCA11350.023.6343611
CAACGAC802.7226633E-823.12533
GCCGTCC1550.022.6774227
GTATCAA29350.021.9982971
AACAACG603.7240883E-521.5833342
CGTCCGC1550.021.48387129
CCGTCCG1650.021.3030328
TTCGCCG1650.021.3030324
CCAACGA909.467476E-820.55555332
TGTCGAC450.003824601420.55555336
ACGACTC909.467476E-820.55555335
TCCGTTT555.140829E-420.18181831
ACAACGC656.898087E-519.9230773
TCTATAC851.2441415E-619.5882363
CGACTCC951.6741978E-719.47368436
CGAGTCG1750.019.02857221
CGCTCTC1850.019.029
GTTTCCG609.2327804E-418.534
CAGCACG701.2184881E-418.59
Produced by FastQC (version 0.11.5)