FastQCFastQC Report
Fri 10 Feb 2017
ERR1633363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849015
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79660.9382637527016602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.6389757542564031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31770.3741983357184502No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.17137506404480485No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10760.1267350989087354No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9760.1149567439915667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG358.8674785E-426.4285716
GGTATCA13300.023.3684221
TCGTTGG502.701297E-422.19999922
AGTCGTT502.701297E-422.19999920
GTATCAA31100.022.0691321
TTCGCCG1850.021.024
ATAACGC450.003824996520.5555553
GCCGCTC1800.019.52777727
ACAACGC609.2341064E-418.53
TTTACCG609.2341064E-418.530
AGACGTA609.2341064E-418.55
CAGTCCG1103.848072E-818.59
TTCGAGG701.21872115E-418.513
GGACCGT1002.87273E-718.4999986
CGGCAGC2400.017.72916617
GCTTCGC2200.017.6590922
TGCGGTA852.7217582E-517.41176436
CCGGCAG2350.017.31914716
GCCGGCA2250.017.26666615
CCGCTCT2050.017.14634128
Produced by FastQC (version 0.11.5)