##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	849015
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7612657020194	33.0	33.0	33.0	27.0	33.0
2	31.913499761488314	33.0	33.0	33.0	33.0	33.0
3	31.63613363721489	33.0	33.0	33.0	27.0	33.0
4	31.923228682649896	33.0	33.0	33.0	33.0	33.0
5	32.01978410275437	33.0	33.0	33.0	33.0	33.0
6	35.15126234518825	37.0	37.0	37.0	33.0	37.0
7	35.294435316219385	37.0	37.0	37.0	33.0	37.0
8	35.439885043256005	37.0	37.0	37.0	33.0	37.0
9	35.56127394686784	37.0	37.0	37.0	33.0	37.0
10	35.564589553777026	37.0	37.0	37.0	33.0	37.0
11	35.57927245101676	37.0	37.0	37.0	33.0	37.0
12	35.56785215808908	37.0	37.0	37.0	33.0	37.0
13	35.56894518942539	37.0	37.0	37.0	33.0	37.0
14	35.55626225685059	37.0	37.0	37.0	33.0	37.0
15	35.57462706783743	37.0	37.0	37.0	33.0	37.0
16	35.55124703332685	37.0	37.0	37.0	33.0	37.0
17	35.54487612115216	37.0	37.0	37.0	33.0	37.0
18	35.539818495550726	37.0	37.0	37.0	33.0	37.0
19	35.51923110899101	37.0	37.0	37.0	33.0	37.0
20	35.52093661478301	37.0	37.0	37.0	33.0	37.0
21	35.51954205756082	37.0	37.0	37.0	33.0	37.0
22	35.427199755010214	37.0	37.0	37.0	33.0	37.0
23	35.46217322426577	37.0	37.0	37.0	33.0	37.0
24	35.45082360146758	37.0	37.0	37.0	33.0	37.0
25	35.478858441841425	37.0	37.0	37.0	33.0	37.0
26	35.363582504431605	37.0	37.0	37.0	33.0	37.0
27	35.36836687219896	37.0	37.0	37.0	33.0	37.0
28	35.38261043680029	37.0	37.0	37.0	33.0	37.0
29	35.3847705870921	37.0	37.0	37.0	33.0	37.0
30	35.3814573358539	37.0	37.0	37.0	33.0	37.0
31	35.36584865991767	37.0	37.0	37.0	33.0	37.0
32	35.36057431258576	37.0	37.0	37.0	33.0	37.0
33	35.35020818242316	37.0	37.0	37.0	33.0	37.0
34	35.30795451199331	37.0	37.0	37.0	33.0	37.0
35	35.23820427200933	37.0	37.0	37.0	33.0	37.0
36	35.2528117877776	37.0	37.0	37.0	33.0	37.0
37	35.24637845032184	37.0	37.0	37.0	33.0	37.0
38	35.217999681984416	37.0	37.0	37.0	33.0	37.0
39	35.10821716930796	37.0	37.0	37.0	33.0	37.0
40	34.88144732425222	37.0	37.0	37.0	27.0	37.0
41	35.03463778614041	37.0	37.0	37.0	27.0	37.0
42	35.06386812953835	37.0	37.0	37.0	33.0	37.0
43	34.704649505603555	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	34.0
16	34.0
17	42.0
18	34.0
19	36.0
20	63.0
21	200.0
22	486.0
23	1105.0
24	2201.0
25	4046.0
26	6306.0
27	9255.0
28	12930.0
29	17217.0
30	22520.0
31	27996.0
32	35558.0
33	46630.0
34	66966.0
35	133314.0
36	462040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.583482035064165	20.82012685288246	14.412701777942674	23.183689334110706
2	15.332237946326035	22.77827835786176	39.79446770669541	22.0950159891168
3	17.695447076906767	28.408214224719234	30.934318003804407	22.962020694569592
4	11.919695176174743	18.52146310724781	40.877605224878245	28.681236491699202
5	13.373379740051709	37.439856775204206	35.08442135886881	14.102342125875278
6	27.344628775698897	41.435899247952044	17.75268988180421	13.466782094544854
7	25.0628080775958	33.562304552923095	23.680853695164398	17.69403367431671
8	24.383550349522682	35.202322691589664	21.1972697773302	19.21685718155745
9	26.277156469555894	15.283593340518129	20.869360376436223	37.56988981348975
10	14.553924253399527	28.489838224295212	34.004346212964435	22.951891309340823
11	32.519331225007804	24.774002815026826	24.65857493683857	18.0480910231268
12	22.4977179437348	26.495762736818552	31.28283952580343	19.72367979364322
13	28.278298969982863	23.154950148112814	26.7385146316614	21.828236250242927
14	21.95120227557817	22.01339198954082	29.600772660082562	26.43463307479844
15	23.330329852829454	29.031760334034146	26.073155362390537	21.564754450745866
16	21.203983439632985	29.468737301461108	26.397060122612675	22.930219136293235
17	21.391848200561828	27.83072148313045	27.51305925101441	23.26437106529331
18	22.70525255737531	26.50848336012909	29.355900661354628	21.430363421140967
19	24.066477035152502	26.503300883965537	29.48699375158272	19.94322832929925
20	23.90028444727125	26.052661024834663	29.288175120580906	20.75887940731318
21	22.380287745210627	27.230967650748223	29.128342844354925	21.260401759686225
22	21.800792683285923	27.034386907180675	29.46767724951856	21.69714316001484
23	21.629653186339464	26.895873453354767	29.798295672043484	21.67617768826228
24	21.769815609853772	27.004234318592722	29.40430970006419	21.821640371489316
25	21.943899695529527	27.00388096794521	29.79912015688769	21.25309917963758
26	22.047667002349783	27.146634629541293	29.262851657509	21.54284671059993
27	21.979352543830203	27.03768484655748	29.35802076523972	21.624941844372596
28	21.603858589070864	27.238270230796864	29.40065841003987	21.7572127700924
29	22.140244871998725	27.1116529154373	29.032231468230833	21.715870744333138
30	22.095722690411833	27.204230785086246	29.440940383856585	21.259106140645336
31	22.159443590513714	26.94487140981019	29.243299588346495	21.6523854113296
32	21.130486504949854	26.953351825350552	29.677214183494993	22.2389474862046
33	21.241674175367926	27.030264482959666	30.093814596915248	21.63424674475716
34	21.488077360235096	26.891868812682933	29.501598911680006	22.118454915401966
35	21.52600366306838	27.495509502187833	29.35837411588723	21.620112718856557
36	21.956855885938413	27.232852187534967	29.394180314835427	21.416111611691193
37	22.237298516516198	27.041453920130976	29.02092424751035	21.700323315842475
38	21.978292491887657	27.19233464661991	28.961914689375334	21.8674581721171
39	21.70915708203035	26.95582527988316	29.277927951802972	22.05708968628352
40	21.71634187852983	27.327550161069002	29.40018727584318	21.55592068455799
41	21.888659210968004	26.783272380346634	29.267562999475867	22.060505409209497
42	21.42188300560061	27.339446299535343	29.356489579100487	21.88218111576356
43	21.66063025977162	27.002820916002662	29.039298481181135	22.297250343044585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	112.5
2	148.0
3	470.5
4	793.0
5	793.0
6	947.0
7	1101.0
8	1085.5
9	1070.0
10	1438.5
11	1807.0
12	1807.0
13	2885.0
14	3963.0
15	7182.5
16	10402.0
17	12062.0
18	13722.0
19	13722.0
20	12759.0
21	11796.0
22	12560.5
23	13325.0
24	16121.5
25	18918.0
26	18918.0
27	22163.0
28	25408.0
29	29308.5
30	33209.0
31	36357.5
32	39506.0
33	39506.0
34	42841.5
35	46177.0
36	48395.0
37	50613.0
38	51749.5
39	52886.0
40	52886.0
41	54720.0
42	56554.0
43	56794.5
44	57035.0
45	57684.5
46	58334.0
47	58334.0
48	58093.0
49	57852.0
50	58560.0
51	59268.0
52	58348.0
53	57428.0
54	57428.0
55	52836.5
56	48245.0
57	43403.5
58	38562.0
59	34302.5
60	30043.0
61	30043.0
62	25886.5
63	21730.0
64	17668.5
65	13607.0
66	11634.0
67	9661.0
68	9661.0
69	7908.0
70	6155.0
71	5208.0
72	4261.0
73	3374.0
74	2487.0
75	2487.0
76	1780.5
77	1074.0
78	852.0
79	630.0
80	517.0
81	404.0
82	404.0
83	338.5
84	273.0
85	261.0
86	249.0
87	198.0
88	147.0
89	147.0
90	109.0
91	71.0
92	45.5
93	20.0
94	12.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	849015.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.12409694384475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30983497387058	56.62788418959408
2	7.350220928097617	9.42652558704016
3	1.5608528053090356	3.0026482980812585
4	0.665050597229753	1.705830758772901
5	0.394910124967737	1.2661627568768505
6	0.250955387353556	0.965537255234372
7	0.18743943803554217	0.841356928698362
8	0.15698536962734036	0.8053236048599098
9	0.11556158443247809	0.6669254018819273
>10	0.8522836248580069	11.012631972284288
>50	0.10245326162347015	4.541882589783837
>100	0.05103914501812874	6.200304377035715
>500	0.00148477512419631	0.663969789096314
>1k	5.567906715736163E-4	0.6793223796669224
>5k	3.711937810490775E-4	1.5936941110931604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7966	0.9382637527016602	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5425	0.6389757542564031	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3177	0.3741983357184502	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1455	0.17137506404480485	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1076	0.1267350989087354	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	976	0.1149567439915667	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.88917745858436E-4	0.0	0.0
2	1.1778354917168719E-4	0.0	5.88917745858436E-4	1.1778354917168719E-4	0.0
3	1.1778354917168719E-4	0.0	5.88917745858436E-4	1.1778354917168719E-4	0.0
4	1.1778354917168719E-4	0.0	7.067012950301232E-4	3.533506475150616E-4	0.0
5	1.1778354917168719E-4	0.0	0.0010600519425451847	3.533506475150616E-4	0.0
6	1.1778354917168719E-4	0.0	0.002709021630948805	3.533506475150616E-4	0.0
7	1.1778354917168719E-4	0.0	0.004946909065210862	4.7113419668674876E-4	0.0
8	2.3556709834337438E-4	0.0	0.006242528106099421	4.7113419668674876E-4	0.0
9	3.533506475150616E-4	0.0	0.007655930696159667	9.422683933734975E-4	0.0
10	7.067012950301232E-4	0.0	0.008480415540361478	0.0010600519425451847	0.0
11	7.067012950301232E-4	0.0	0.012013922015512093	0.001295619040888559	0.0
12	8.244848442018103E-4	0.0	0.013662891703915714	0.0014134025900602463	0.0
13	8.244848442018103E-4	0.0	0.014605160097289212	0.0014134025900602463	0.0
14	8.244848442018103E-4	0.0	0.015547428490662709	0.0015311861392319334	0.0
15	8.244848442018103E-4	0.0	0.017431965277409704	0.0015311861392319334	0.0
16	8.244848442018103E-4	0.0	0.01896315141664164	0.0015311861392319334	0.0
17	8.244848442018103E-4	0.0	0.019198718514985013	0.0016489696884036207	0.0
18	8.244848442018103E-4	0.0	0.019434285613328387	0.0016489696884036207	0.0
19	8.244848442018103E-4	0.0	0.019552069162500074	0.0017667532375753078	0.0
20	0.001177835491716872	0.0	0.019905419810015135	0.001884536786746995	0.0
21	0.001295619040888559	0.0	0.020847688203388632	0.002473454532605431	0.0
22	0.001295619040888559	0.0	0.02096547175256032	0.0031801558276355543	0.0
23	0.001295619040888559	0.0	0.02096547175256032	0.004122424221009052	0.0
24	0.001295619040888559	0.0	0.021201038850903693	0.00541804326189761	0.0
25	0.0014134025900602463	0.0	0.021201038850903693	0.006242528106099421	0.0
26	0.0014134025900602463	0.0	0.02131882240007538	0.007184796499472919	0.0
27	0.0014134025900602463	0.0	0.02131882240007538	0.0091871168353916	0.0
28	0.0014134025900602463	0.0	0.02131882240007538	0.021554389498418754	0.0
29	0.0014134025900602463	0.0	0.021436605949247067	0.04840903870956344	0.0
30	0.0014134025900602463	0.0	0.021436605949247067	0.08539307314947321	0.0
31	0.0014134025900602463	0.0	0.021436605949247067	0.15429644941491022	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATCG	35	8.8674785E-4	26.42857	16
GGTATCA	1330	0.0	23.368422	1
TCGTTGG	50	2.701297E-4	22.199999	22
AGTCGTT	50	2.701297E-4	22.199999	20
GTATCAA	3110	0.0	22.069132	1
TTCGCCG	185	0.0	21.0	24
ATAACGC	45	0.0038249965	20.555555	3
GCCGCTC	180	0.0	19.527777	27
ACAACGC	60	9.2341064E-4	18.5	3
TTTACCG	60	9.2341064E-4	18.5	30
AGACGTA	60	9.2341064E-4	18.5	5
CAGTCCG	110	3.848072E-8	18.5	9
TTCGAGG	70	1.21872115E-4	18.5	13
GGACCGT	100	2.87273E-7	18.499998	6
CGGCAGC	240	0.0	17.729166	17
GCTTCGC	220	0.0	17.65909	22
TGCGGTA	85	2.7217582E-5	17.411764	36
CCGGCAG	235	0.0	17.319147	16
GCCGGCA	225	0.0	17.266666	15
CCGCTCT	205	0.0	17.146341	28
>>END_MODULE