FastQCFastQC Report
Fri 10 Feb 2017
ERR1633362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930428
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37870.40701698573129785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.30588073445769043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14920.1603563091394498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG358.868118E-426.4285724
GGTATCA7100.023.971831
GTATCAA17350.021.9654161
TTTCGGG957.1413524E-921.42105322
TTATCGG450.003825269220.55555525
ATAGAAC801.6161304E-518.53
TTCGGGG1205.169568E-918.523
TTCGCCG1409.458745E-1118.524
GACCGTT500.007033900418.4999987
CGTAAAT500.007033900418.49999815
GTTATAC500.007033900418.4999983
TTAACGG1757.2759576E-1216.91428635
AGCTTCG1554.0017767E-1016.70967921
GTATTAG3900.016.6025621
TAACGGC1801.0913936E-1116.44444536
CCGCTCT1801.0913936E-1116.44444528
GCCGCTC1801.0913936E-1116.44444527
TAGGACC2150.016.3488394
GCCGGCA1606.2937033E-1016.187515
GCTTCGC1606.2937033E-1016.187522
Produced by FastQC (version 0.11.5)