FastQCFastQC Report
Fri 10 Feb 2017
ERR1633361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713185
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26430.3705910808555985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.2749637190911194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12080.1693810161458808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT250.005494961529.619
GGTATCA4350.023.8160931
GTATCAA12800.023.7031251
GGTCGCC1400.022.46428536
TATGCGG705.097443E-621.1428575
CGAACCG555.140463E-420.18181831
GTCGCCC1403.6379788E-1219.82142837
TTATGCG759.2576865E-619.7333344
GCTCGGA1750.019.0285711
TGCTCGG1950.018.97435810
GGGTATA801.6152688E-518.51
AGGTCGC1552.0008883E-1117.90322735
GTATAGA1258.561983E-917.761
TGTAGGG852.7207698E-517.4117642
TCGGAGG1603.45608E-1117.3437513
CTGCTCG2350.017.3191499
TTCCGAA752.0658805E-417.26666616
CTCGGAG1950.017.07692112
CCGTGCA1201.03953425E-716.95833410
ATCAACG17600.016.7130683
Produced by FastQC (version 0.11.5)