##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633361.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713185
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.641223525452723	33.0	33.0	33.0	27.0	33.0
2	31.8206986966916	33.0	33.0	33.0	27.0	33.0
3	31.543762137453815	33.0	33.0	33.0	27.0	33.0
4	31.83554617665823	33.0	33.0	33.0	27.0	33.0
5	31.950506530563597	33.0	33.0	33.0	33.0	33.0
6	35.05502359135428	37.0	37.0	37.0	33.0	37.0
7	35.19556496561201	37.0	37.0	37.0	33.0	37.0
8	35.30148699145383	37.0	37.0	37.0	33.0	37.0
9	35.43906139360755	37.0	37.0	37.0	33.0	37.0
10	35.47240197143799	37.0	37.0	37.0	33.0	37.0
11	35.464910226659285	37.0	37.0	37.0	33.0	37.0
12	35.46696158780681	37.0	37.0	37.0	33.0	37.0
13	35.46223069750486	37.0	37.0	37.0	33.0	37.0
14	35.44537111689113	37.0	37.0	37.0	33.0	37.0
15	35.44770571450606	37.0	37.0	37.0	33.0	37.0
16	35.428723262547585	37.0	37.0	37.0	33.0	37.0
17	35.43090362248225	37.0	37.0	37.0	33.0	37.0
18	35.41914229828165	37.0	37.0	37.0	33.0	37.0
19	35.42006772436324	37.0	37.0	37.0	33.0	37.0
20	35.40982634239363	37.0	37.0	37.0	33.0	37.0
21	35.409679115517015	37.0	37.0	37.0	33.0	37.0
22	35.31345162896023	37.0	37.0	37.0	33.0	37.0
23	35.35879330047603	37.0	37.0	37.0	33.0	37.0
24	35.34459502092725	37.0	37.0	37.0	33.0	37.0
25	35.362368810336726	37.0	37.0	37.0	33.0	37.0
26	35.250676893092255	37.0	37.0	37.0	33.0	37.0
27	35.26195447184111	37.0	37.0	37.0	33.0	37.0
28	35.275289020380406	37.0	37.0	37.0	33.0	37.0
29	35.28676430379215	37.0	37.0	37.0	33.0	37.0
30	35.279025778725014	37.0	37.0	37.0	33.0	37.0
31	35.27925292876322	37.0	37.0	37.0	33.0	37.0
32	35.26695878348535	37.0	37.0	37.0	33.0	37.0
33	35.258801012360045	37.0	37.0	37.0	33.0	37.0
34	35.237617168055976	37.0	37.0	37.0	33.0	37.0
35	35.167551196393646	37.0	37.0	37.0	33.0	37.0
36	35.21304009478607	37.0	37.0	37.0	33.0	37.0
37	35.200726319257974	37.0	37.0	37.0	33.0	37.0
38	35.19819962562308	37.0	37.0	37.0	33.0	37.0
39	35.09156249780912	37.0	37.0	37.0	33.0	37.0
40	34.89803346957662	37.0	37.0	37.0	27.0	37.0
41	35.06103185008097	37.0	37.0	37.0	27.0	37.0
42	35.10826503642112	37.0	37.0	37.0	33.0	37.0
43	34.76193554267125	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	29.0
17	25.0
18	23.0
19	34.0
20	82.0
21	151.0
22	453.0
23	1069.0
24	2105.0
25	3696.0
26	5834.0
27	8393.0
28	11729.0
29	15251.0
30	19461.0
31	24806.0
32	30804.0
33	40703.0
34	58377.0
35	116195.0
36	373951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17288641797009	21.32041475914384	14.18888507189579	24.317813750990275
2	15.440453739212126	21.973401010957886	39.169920848026806	23.41622440180318
3	17.58001079663762	27.631259771307587	30.24110153746924	24.547627894585556
4	12.35471862139557	18.43182343992092	40.04991692197677	29.163541016706745
5	13.544592216605789	37.5258873924718	35.73532814066476	13.194192250257647
6	28.944383294657065	40.3843322560065	17.881194921373837	12.79008952796259
7	25.781529336707866	32.562799273680746	24.209006078366762	17.446665311244626
8	24.0199948120053	36.77769442711218	20.878033048928398	18.32427771195412
9	25.251652796960116	16.08362486591838	20.883501475774167	37.781220861347336
10	14.2474953903966	28.850578741841176	34.689877100612044	22.21204876715018
11	33.20050197354122	24.139458906174415	24.954254506194047	17.705784614090312
12	22.200551049166766	26.100941550929985	31.34642484067949	20.352082559223764
13	27.288992337191615	22.808387725484973	26.764444008216664	23.13817592910675
14	21.274143455064255	21.53396383827478	28.52149161858424	28.670401088076723
15	22.933740894718763	29.723143363923803	24.686582022897284	22.656533718460146
16	22.253132076529933	29.291418075253965	25.75699152393839	22.698458324277713
17	21.687079789956325	28.520930754292362	27.239776495579687	22.552212960171623
18	22.251028835435406	28.048402588388704	28.343978070206187	21.3565905059697
19	23.537791737066822	27.379291488183288	28.108134635473263	20.974782139276627
20	23.102140398353864	27.330496294790272	28.221429222431766	21.345934084424098
21	22.36796904029109	27.583305874352376	28.28242321417304	21.766301871183494
22	22.04687423319335	27.542222564972622	28.31944025743671	22.091462944397318
23	22.12553545012865	27.33736688236573	28.37791035986455	22.159187307641076
24	22.135490791309408	27.8271416252445	27.772036708567903	22.265330874878188
25	22.40204154602242	27.467767830226382	28.190020822086836	21.940169801664364
26	22.334737830997568	27.667295302060474	28.126783373178064	21.87118349376389
27	22.13885597706065	27.786338748010685	27.764605256700577	22.31020001822809
28	21.964006534069	27.5667603777421	28.29406114822942	22.17517193995948
29	22.72930586033077	27.49581104482007	27.751424945841542	22.02345814900762
30	22.552072744098655	27.316755119639364	28.131270287513054	21.999901848748923
31	22.323380329087122	27.35545475577866	28.179224184468264	22.141940730665958
32	21.89880606013867	27.2535176707306	28.292658987499735	22.55501728163099
33	21.87847472955825	27.405792325974325	28.53957949199717	22.176153452470256
34	22.311181530738867	27.18102596100591	28.328834734325596	22.178957773929625
35	22.323380329087122	27.609526279997475	28.0373255186242	22.0297678722912
36	22.710937554771903	27.548952936475107	27.77764535148664	21.962464157266346
37	22.398255712052272	27.11736786387824	27.968058778577788	22.5163176454917
38	22.32001514333588	27.499877310936157	27.95614041237547	22.223967133352495
39	22.103241094526666	27.39751957766919	28.10490966579499	22.394329662009156
40	22.187791386526637	27.54376494177528	28.40006449939357	21.86837917230452
41	22.102399798088854	27.167144569782035	28.30513821799393	22.425317414135183
42	21.91002334597615	27.743011981463432	28.169268843287504	22.17769582927291
43	22.372736386772015	27.05300868638572	28.16968949150641	22.40456543533585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	41.0
2	55.0
3	172.0
4	289.0
5	289.0
6	363.0
7	437.0
8	397.5
9	358.0
10	511.5
11	665.0
12	665.0
13	1040.0
14	1415.0
15	2697.0
16	3979.0
17	4758.0
18	5537.0
19	5537.0
20	5984.0
21	6431.0
22	7638.5
23	8846.0
24	11480.0
25	14114.0
26	14114.0
27	17312.0
28	20510.0
29	23943.5
30	27377.0
31	30742.0
32	34107.0
33	34107.0
34	38747.5
35	43388.0
36	45969.5
37	48551.0
38	50513.5
39	52476.0
40	52476.0
41	53610.0
42	54744.0
43	54818.5
44	54893.0
45	53543.0
46	52193.0
47	52193.0
48	50148.0
49	48103.0
50	47257.5
51	46412.0
52	43933.0
53	41454.0
54	41454.0
55	39230.5
56	37007.0
57	33094.5
58	29182.0
59	26949.5
60	24717.0
61	24717.0
62	21787.0
63	18857.0
64	15915.5
65	12974.0
66	10873.0
67	8772.0
68	8772.0
69	7311.0
70	5850.0
71	4831.0
72	3812.0
73	3090.5
74	2369.0
75	2369.0
76	1841.0
77	1313.0
78	1042.0
79	771.0
80	649.0
81	527.0
82	527.0
83	395.0
84	263.0
85	253.0
86	243.0
87	168.5
88	94.0
89	94.0
90	75.5
91	57.0
92	35.0
93	13.0
94	8.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	713185.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.48338965763675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.35926900739518	64.04579325326971
2	7.334117669644699	10.63203417687631
3	1.7902644151881115	3.892932995888432
4	0.7411961206593859	2.1489762890593203
5	0.4440233379946813	1.6092158312476512
6	0.2667029352433611	1.1598919666848
7	0.1823183090335892	0.9250534324782156
8	0.1380337536567213	0.8004123481765095
9	0.1101449174928387	0.7185309276097508
>10	0.5606914087008797	7.502536440940939
>50	0.047721973120581615	2.39605335547165
>100	0.02473703272965375	3.2683862342059777
>500	1.9477978509392714E-4	0.0796272067006214
>1k	5.843393552817814E-4	0.8205555413900915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2643	0.3705910808555985	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1961	0.2749637190911194	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1208	0.1693810161458808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.8043214593688877E-4	0.0	0.0
4	0.0	0.0	4.206482189053331E-4	0.0	0.0
5	0.0	0.0	4.206482189053331E-4	0.0	0.0
6	0.0	0.0	7.010803648422218E-4	0.0	0.0
7	0.0	0.0	0.001121728583747555	0.0	0.0
8	0.0	0.0	0.0012619446567159994	0.0	0.0
9	0.0	0.0	0.0012619446567159994	4.206482189053331E-4	0.0
10	1.4021607296844438E-4	0.0	0.0015423768026528882	5.608642918737775E-4	0.0
11	1.4021607296844438E-4	0.0	0.0026641053864004433	9.815125107791106E-4	0.0
12	1.4021607296844438E-4	0.0	0.002944537532337332	9.815125107791106E-4	0.0
13	1.4021607296844438E-4	0.0	0.0030847536053057764	9.815125107791106E-4	0.0
14	1.4021607296844438E-4	0.0	0.0036456178971795536	9.815125107791106E-4	0.0
15	1.4021607296844438E-4	0.0	0.0036456178971795536	0.001121728583747555	0.0
16	1.4021607296844438E-4	0.0	0.003785833970147998	0.001121728583747555	0.0
17	1.4021607296844438E-4	0.0	0.004206482189053331	0.0012619446567159994	0.0
18	1.4021607296844438E-4	0.0	0.004627130407958664	0.0014021607296844437	0.0
19	1.4021607296844438E-4	0.0	0.004627130407958664	0.0014021607296844437	0.0
20	1.4021607296844438E-4	0.0	0.0047673464809271085	0.0015423768026528882	0.0
21	1.4021607296844438E-4	0.0	0.005187994699832442	0.0015423768026528882	0.0
22	1.4021607296844438E-4	0.0	0.0053282107728008865	0.00224345716749511	0.0
23	1.4021607296844438E-4	0.0	0.0053282107728008865	0.0025238893134319988	0.0
24	1.4021607296844438E-4	0.0	0.0053282107728008865	0.0028043214593688874	0.0
25	1.4021607296844438E-4	0.0	0.0053282107728008865	0.0030847536053057764	0.0
26	1.4021607296844438E-4	0.0	0.0053282107728008865	0.0032249696782742205	0.0
27	1.4021607296844438E-4	0.0	0.0053282107728008865	0.003926050043116442	0.0
28	1.4021607296844438E-4	0.0	0.0053282107728008865	0.00897382866998044	0.0
29	1.4021607296844438E-4	0.0	0.0053282107728008865	0.019910682361519102	0.0
30	1.4021607296844438E-4	0.0	0.0053282107728008865	0.03463337002320576	0.0
31	1.4021607296844438E-4	0.0	0.0053282107728008865	0.0745949508192124	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGAT	25	0.0054949615	29.6	19
GGTATCA	435	0.0	23.816093	1
GTATCAA	1280	0.0	23.703125	1
GGTCGCC	140	0.0	22.464285	36
TATGCGG	70	5.097443E-6	21.142857	5
CGAACCG	55	5.140463E-4	20.181818	31
GTCGCCC	140	3.6379788E-12	19.821428	37
TTATGCG	75	9.2576865E-6	19.733334	4
GCTCGGA	175	0.0	19.02857	11
TGCTCGG	195	0.0	18.974358	10
GGGTATA	80	1.6152688E-5	18.5	1
AGGTCGC	155	2.0008883E-11	17.903227	35
GTATAGA	125	8.561983E-9	17.76	1
TGTAGGG	85	2.7207698E-5	17.411764	2
TCGGAGG	160	3.45608E-11	17.34375	13
CTGCTCG	235	0.0	17.319149	9
TTCCGAA	75	2.0658805E-4	17.266666	16
CTCGGAG	195	0.0	17.076921	12
CCGTGCA	120	1.03953425E-7	16.958334	10
ATCAACG	1760	0.0	16.713068	3
>>END_MODULE