FastQCFastQC Report
Fri 10 Feb 2017
ERR1633360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660711
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179522.717072971389912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT137102.0750373461316673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75691.1455840753370232No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA39870.6034408387328196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.5127809284240765No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31550.47751588818711965No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG29220.4422508479501628No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28790.43574270747724797No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT27880.4219696660113121No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC24080.3644558664832279No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23580.3568882612821642No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18310.2771257024629528No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC18070.2734932519664422No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.27213108303025074No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG17660.26728781570156995No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17080.2585093936683361No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16720.2530607179235702No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16710.2529093658195489No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16530.250185027947166No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA16280.24640122534663414No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA15910.240801197497847No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15310.23172007125657057No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15130.22899573338418766No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14560.22036866345497502No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14510.21961190293486865No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC14340.21703891716650697No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13710.20750373461316673No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13380.20250911518046466No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA13340.2019037067643796No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC13040.19736314364374136No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12990.196606383123635No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12820.19403339735527333No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC12440.18828201740246492No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11860.17950359536923102No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT11850.17935224326520977No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT11850.17935224326520977No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11730.17753601801695446No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11720.1773846659129332No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11680.17677925749684809No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11630.17602249697674174No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA11530.17450897593652898No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11440.17314680700033752No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.17057382123197587No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC11110.16815218756763547No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT11060.1673954270475291No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT11010.16663866652742274No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10770.16300621603091214No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10560.1598278218464654No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10490.1587683571183165No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.157406188182125No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10360.15680077976603993No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT10070.15241156874942297No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA10020.1516548082293166No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10000.15135210402127405No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG9980.1510493998132315No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT9840.14893047035693366No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9820.14862776614889112No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA9810.14847641404486983No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9760.14771965352476346No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA9750.14756830142074218No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG9720.14711424510867838No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA9600.1452980198604231No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9370.1418169214679338No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC9320.14106016094782742No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG9220.13954663990761468No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9100.13773041465935937No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9000.13621689361914666No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8920.13500607678697646No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8830.13364390785078498No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA8830.13364390785078498No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8820.13349255574676372No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT8630.1306168657703595No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.12940604893818933No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8540.12925469683416804No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8520.1289519926261255No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA8380.12683306316982765No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG8350.12637900685776382No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC8260.12501683792157237No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8020.12138438742506179No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7950.12032492269691288No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG7950.12032492269691288No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC7920.11987086638484905No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7910.11971951428082776No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT7900.1195681621768065No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG7740.11714652851246611No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT7590.11487624695214702No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG7540.11411948643204063No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7540.11411948643204063No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7460.11290866959987045No Hit
GTATCAACGCAGAGTACGGGACTAGTATGAAACTGCTTGTCCG7280.11018433172748751No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA7240.10957892331140241No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7200.10897351489531733No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG7150.10821675437521094No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7050.1067032333349982No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA7010.1060978249189131No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6990.10579512071087055No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6970.10549241650282802No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6950.10518971229478546No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6930.10488700808674292No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6930.10488700808674292No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC6830.10337348704653017No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC6810.10307078283848764No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA6710.10155726179827489No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA6670.1009518533821898No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6620.10019509286208342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCT200.001841370137.037
TAGCAAC200.001841370137.01
CTTCCCT303.5978368E-430.83333235
CAACGAC250.005494673729.59999820
TAAGTTG250.005494673729.59999817
TAACAAC405.9363672E-527.751
GGTATCA28700.026.106271
ATCTCGT509.083453E-625.89999837
ACCGAGT451.3223803E-424.66666822
CCGAGTC451.3223803E-424.66666823
GACGTAG601.3359695E-624.66666626
AGACGTA601.3359695E-624.66666625
GAGACGT601.3359695E-624.66666624
TATTATA701.9203435E-723.7857132
TCCCGTG400.001930323423.12522
TATTAGC1054.0017767E-1122.904762
GGTCGCT652.679979E-622.7692328
TCCGAGC652.679979E-622.7692313
AGCCCAC652.679979E-622.7692317
CGCTCTC6500.022.7692329
Produced by FastQC (version 0.11.5)