FastQCFastQC Report
Fri 10 Feb 2017
ERR1633359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780685
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53560.6860641616016704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.5204403824846129No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21470.27501489076900415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.13782767697598902No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG10210.13078258196327586No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA9120.11682048457444423No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8210.10516405464431877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCA1450.029.34482815
GGTATCA6650.029.2105271
GCTTCGC1750.026.42857222
GTATCAA21050.025.1353911
GCCGCTC1550.025.06451427
CCGGCAG1700.023.94117716
TTCGCCG1650.023.54545424
AGCTTCG1950.022.76922821
TGCTCGC2000.022.19999910
CGCCGGC1950.021.8205114
GCGGATA603.7243146E-521.58333419
TACCGGG603.7243146E-521.58333432
CCGCTCT1850.021.028
ACGGGTC1001.2882083E-820.34999816
CGAGTCG1101.7516868E-920.18181821
CGCTCTC1950.019.92307529
CTGCTCG3500.019.5571449
TCGCCGC2300.018.525
CTTAACG500.00703291218.49999817
GGACCGT500.00703291218.4999986
Produced by FastQC (version 0.11.5)