##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	780685
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.667908311290727	33.0	33.0	33.0	27.0	33.0
2	31.82261731684354	33.0	33.0	33.0	27.0	33.0
3	31.55753344818973	33.0	33.0	33.0	27.0	33.0
4	31.853658005469555	33.0	33.0	33.0	27.0	33.0
5	31.957071033771623	33.0	33.0	33.0	33.0	33.0
6	35.08139902777689	37.0	37.0	37.0	33.0	37.0
7	35.221515720168824	37.0	37.0	37.0	33.0	37.0
8	35.357348994793036	37.0	37.0	37.0	33.0	37.0
9	35.47935338837047	37.0	37.0	37.0	33.0	37.0
10	35.49621678397817	37.0	37.0	37.0	33.0	37.0
11	35.5002670731473	37.0	37.0	37.0	33.0	37.0
12	35.471195168345744	37.0	37.0	37.0	33.0	37.0
13	35.483413924950526	37.0	37.0	37.0	33.0	37.0
14	35.461220594734115	37.0	37.0	37.0	33.0	37.0
15	35.486289604642074	37.0	37.0	37.0	33.0	37.0
16	35.463232930055014	37.0	37.0	37.0	33.0	37.0
17	35.44941429641917	37.0	37.0	37.0	33.0	37.0
18	35.44553565138308	37.0	37.0	37.0	33.0	37.0
19	35.438653234018844	37.0	37.0	37.0	33.0	37.0
20	35.436790767082755	37.0	37.0	37.0	33.0	37.0
21	35.4263729929485	37.0	37.0	37.0	33.0	37.0
22	35.32467384412407	37.0	37.0	37.0	33.0	37.0
23	35.376752467384414	37.0	37.0	37.0	33.0	37.0
24	35.36595425811947	37.0	37.0	37.0	33.0	37.0
25	35.38962705828855	37.0	37.0	37.0	33.0	37.0
26	35.26158181596931	37.0	37.0	37.0	33.0	37.0
27	35.27416563658838	37.0	37.0	37.0	33.0	37.0
28	35.28649327193426	37.0	37.0	37.0	33.0	37.0
29	35.29343717376407	37.0	37.0	37.0	33.0	37.0
30	35.292659651459935	37.0	37.0	37.0	33.0	37.0
31	35.28289771162505	37.0	37.0	37.0	33.0	37.0
32	35.274825313666845	37.0	37.0	37.0	33.0	37.0
33	35.262057039651076	37.0	37.0	37.0	33.0	37.0
34	35.24116128784337	37.0	37.0	37.0	33.0	37.0
35	35.162757065910064	37.0	37.0	37.0	33.0	37.0
36	35.18889821118633	37.0	37.0	37.0	33.0	37.0
37	35.17916957543696	37.0	37.0	37.0	33.0	37.0
38	35.16493976443764	37.0	37.0	37.0	33.0	37.0
39	35.05825268834421	37.0	37.0	37.0	33.0	37.0
40	34.83716992128708	37.0	37.0	37.0	27.0	37.0
41	35.00497511800534	37.0	37.0	37.0	27.0	37.0
42	35.03956781544413	37.0	37.0	37.0	27.0	37.0
43	34.70422769747081	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	20.0
16	27.0
17	28.0
18	30.0
19	33.0
20	66.0
21	201.0
22	512.0
23	1114.0
24	2204.0
25	3808.0
26	6264.0
27	9123.0
28	12605.0
29	16793.0
30	21311.0
31	27170.0
32	34178.0
33	44744.0
34	64285.0
35	125913.0
36	410255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34740644434054	21.191773890877883	13.966580631112421	23.49423903366915
2	15.711714712079777	22.78678340175615	39.215176415583755	22.286325470580323
3	17.483107783549062	28.170388825198383	30.606582680594606	23.739920710657948
4	11.854717331574193	18.59034053427439	40.88704150841889	28.66790062573253
5	13.27270281867847	37.466840018701525	35.77512056719419	13.485336595425812
6	27.79008178714847	41.519050577377556	17.82703651280606	12.863831122667913
7	25.746107585005472	32.880226980152045	24.03607088646509	17.337594548377385
8	24.019290751071175	35.798689612327635	21.235581572593297	18.94643806400789
9	25.6276218961553	15.155920761895004	21.099291007256447	38.117166334693245
10	14.522630766570382	28.5654265164567	34.68658934141171	22.225353375561205
11	32.99845648372903	24.57777464662444	24.651556005303036	17.77221286434349
12	22.080864881482288	26.506849753741907	31.252681939578704	20.159603425197105
13	27.595509072160983	23.06705009062554	26.968239430756324	22.36920140645715
14	21.600645586888437	21.745646451513736	28.919346471368097	27.734361490229738
15	22.937676527664806	29.677270602099437	25.8093853474833	21.575667522752454
16	22.191408826863587	29.14965703196552	25.9689887726804	22.68994536849049
17	21.82532007147569	27.842471675515736	27.125409095858128	23.206799157150453
18	22.17539724728924	27.18305078232578	29.111997796806648	21.529554173578333
19	24.321205095525084	26.81350352574982	28.70722506516713	20.158066313557967
20	23.6575571453275	26.882033086328033	28.661367901266193	20.799041867078273
21	22.093930330414956	27.619334302567616	28.70889026944286	21.577845097574567
22	22.251099995516757	26.878062214593594	28.966612654271568	21.90422513561808
23	21.60179842061779	27.18305078232578	29.534575404932852	21.680575392123583
24	21.775235850567128	27.822233038933756	28.32358761856575	22.078943491933366
25	22.03218967957627	27.703747350083578	29.21741803672416	21.04664493361599
26	22.624361938553964	27.348674561442838	28.397881347790722	21.62908215221248
27	22.20677994325496	27.487911257421366	28.04524231924528	22.260066480078393
28	21.79368119023678	27.166398739568454	29.23009920774704	21.809820862447722
29	22.744640924316464	27.47996951395249	28.432210174398126	21.34317938733292
30	22.557753767524673	27.1407802122495	28.58489659721911	21.716569423006717
31	22.155286703343858	26.965549485387836	28.806624951164682	22.072538860103627
32	21.35662911417537	27.19867808399034	29.042699680408873	22.401993121425416
33	21.50841888854019	26.950306461633055	29.353836694697605	22.18743795512915
34	22.106995779347624	27.032541934326904	29.073313820555025	21.787148465770446
35	22.104049648705946	27.6099835400962	28.317951542555576	21.968015268642283
36	22.25353375561206	27.27450892485446	28.19523879669777	22.276718522835715
37	22.321679038280486	26.951971665908786	28.584384226672732	22.141965069138
38	22.061266708083284	27.147312936715835	28.24788487033823	22.54353548486265
39	21.854525192619302	27.131685635051266	28.75397887752423	22.259810294805202
40	22.05460589098036	27.61702863510891	28.804959746888947	21.523405727021782
41	22.124672563197706	26.881008345235273	28.597193490332206	22.397125601234812
42	21.316279933648012	28.374568487930475	28.30462990834972	22.004521670071796
43	22.391105247314858	26.6348142977001	28.406847832352355	22.567232622632687
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	76.0
2	104.0
3	360.5
4	617.0
5	617.0
6	738.5
7	860.0
8	802.0
9	744.0
10	1042.0
11	1340.0
12	1340.0
13	2198.5
14	3057.0
15	5355.5
16	7654.0
17	8677.5
18	9701.0
19	9701.0
20	9339.5
21	8978.0
22	9900.0
23	10822.0
24	13209.0
25	15596.0
26	15596.0
27	18507.5
28	21419.0
29	24459.0
30	27499.0
31	30536.5
32	33574.0
33	33574.0
34	39720.5
35	45867.0
36	48760.5
37	51654.0
38	52984.5
39	54315.0
40	54315.0
41	55498.5
42	56682.0
43	57735.0
44	58788.0
45	57276.5
46	55765.0
47	55765.0
48	53947.5
49	52130.0
50	52232.5
51	52335.0
52	51338.0
53	50341.0
54	50341.0
55	47840.5
56	45340.0
57	38657.5
58	31975.0
59	29496.5
60	27018.0
61	27018.0
62	23880.0
63	20742.0
64	16180.0
65	11618.0
66	9904.5
67	8191.0
68	8191.0
69	6831.0
70	5471.0
71	5104.5
72	4738.0
73	4104.0
74	3470.0
75	3470.0
76	2214.5
77	959.0
78	733.0
79	507.0
80	411.0
81	315.0
82	315.0
83	249.5
84	184.0
85	156.0
86	128.0
87	110.5
88	93.0
89	93.0
90	65.0
91	37.0
92	21.5
93	6.0
94	4.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	780685.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.731824093913396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4318103423297	51.35029705238105
2	7.624188294430103	8.955649715346846
3	1.6846116345868654	2.9682094256734706
4	0.7507280250268438	1.763665052329904
5	0.45139403100361514	1.3255597412973406
6	0.2985758758336937	1.0521543490890375
7	0.2205642471764248	0.9067898396600552
8	0.17535286627779836	0.8239034957272937
9	0.13482731981467125	0.7126788987368168
>10	1.0018775414484653	11.776393540985683
>50	0.12927775829283011	5.302307192088922
>100	0.09170937549871627	9.830506561809942
>500	0.003977756045444774	1.4585729547291053
>1k	8.839457878766164E-4	1.0781603074332335
>5k	2.209864469691541E-4	0.6951518727112553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5356	0.6860641616016704	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4063	0.5204403824846129	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2147	0.27501489076900415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.13782767697598902	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1021	0.13078258196327586	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	912	0.11682048457444423	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	821	0.10516405464431877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	5.123705463791414E-4	0.0	0.0
2	0.0	0.0	5.123705463791414E-4	0.0	0.0
3	0.0	0.0	6.404631829739268E-4	0.0	0.0
4	0.0	0.0	7.685558195687121E-4	0.0	0.0
5	0.0	0.0	7.685558195687121E-4	0.0	0.0
6	0.0	0.0	0.002177574822111351	0.0	0.0
7	0.0	0.0	0.00550798337357577	2.561852731895707E-4	0.0
8	1.2809263659478536E-4	0.0	0.005764168646765341	2.561852731895707E-4	0.0
9	1.2809263659478536E-4	0.0	0.007557465559092336	8.966484561634975E-4	0.0
10	6.404631829739268E-4	0.0	0.008069836105471477	0.0011528337293530682	0.0
11	6.404631829739268E-4	0.0	0.01101596674715154	0.0015371116391374243	0.0
12	6.404631829739268E-4	0.0	0.01242498574969418	0.0015371116391374243	0.0
13	6.404631829739268E-4	0.0	0.013577819479047248	0.0015371116391374243	0.0
14	6.404631829739268E-4	0.0	0.01460256057180553	0.0015371116391374243	0.0
15	6.404631829739268E-4	0.0	0.015243023754779457	0.0015371116391374243	0.0
16	6.404631829739268E-4	0.0	0.016395857484132526	0.0019213895489217803	0.0
17	6.404631829739268E-4	0.0	0.016652042757322096	0.0020494821855165657	0.0
18	6.404631829739268E-4	0.0	0.016908228030511667	0.002177574822111351	0.0
19	6.404631829739268E-4	0.0	0.01703632066710645	0.0023056674587061364	0.0
20	6.404631829739268E-4	0.0	0.01729250594029602	0.0024337600953009218	0.0
21	6.404631829739268E-4	0.0	0.01844533966964909	0.0026899453684904925	0.0
22	6.404631829739268E-4	0.0	0.018829617579433446	0.003202315914869634	0.0
23	6.404631829739268E-4	0.0	0.018829617579433446	0.0038427790978435607	0.0
24	6.404631829739268E-4	0.0	0.018829617579433446	0.004098964371033131	0.0
25	6.404631829739268E-4	0.0	0.018829617579433446	0.0044832422808174874	0.0
26	6.404631829739268E-4	0.0	0.018829617579433446	0.0052517981003862	0.0
27	6.404631829739268E-4	0.0	0.018829617579433446	0.006532724466334053	0.0
28	6.404631829739268E-4	0.0	0.018829617579433446	0.01742059857689081	0.0
29	6.404631829739268E-4	0.0	0.018829617579433446	0.04073345843714174	0.0
30	6.404631829739268E-4	0.0	0.018829617579433446	0.06686435630247795	0.0
31	6.404631829739268E-4	1.2809263659478536E-4	0.018957710216028233	0.13052639669008628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGCA	145	0.0	29.344828	15
GGTATCA	665	0.0	29.210527	1
GCTTCGC	175	0.0	26.428572	22
GTATCAA	2105	0.0	25.135391	1
GCCGCTC	155	0.0	25.064514	27
CCGGCAG	170	0.0	23.941177	16
TTCGCCG	165	0.0	23.545454	24
AGCTTCG	195	0.0	22.769228	21
TGCTCGC	200	0.0	22.199999	10
CGCCGGC	195	0.0	21.82051	14
GCGGATA	60	3.7243146E-5	21.583334	19
TACCGGG	60	3.7243146E-5	21.583334	32
CCGCTCT	185	0.0	21.0	28
ACGGGTC	100	1.2882083E-8	20.349998	16
CGAGTCG	110	1.7516868E-9	20.181818	21
CGCTCTC	195	0.0	19.923075	29
CTGCTCG	350	0.0	19.557144	9
TCGCCGC	230	0.0	18.5	25
CTTAACG	50	0.007032912	18.499998	17
GGACCGT	50	0.007032912	18.499998	6
>>END_MODULE