FastQCFastQC Report
Fri 10 Feb 2017
ERR1633358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834870
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31760.38041850827074875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.25572843676260976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12080.1446931857654485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.024.6666661
GTATCAA18600.023.2741951
CGCCGCT1705.456968E-1217.41176426
CCGCTCT1401.8662831E-917.17857228
GGTCGCC1757.2759576E-1216.91428636
AGGTCGC1655.4569682E-1116.81818235
GCCGCTC1554.0017767E-1016.70967927
TTCGCCG1554.0017767E-1016.70967924
TATCAAC26650.016.0356462
ATCAACG26750.015.9757013
TCAACGC27150.015.9447524
CCTACCG1059.336898E-615.85714224
AACGGCC1403.468631E-815.85714237
GTAGTAT1059.336898E-615.8571423
CAACGCA27550.015.7132495
AACGCAG27900.015.5161296
GCGACAA1201.9338586E-615.41666716
GTCGCCC1802.0190782E-1015.41666637
TGCTCGG2055.456968E-1215.34146410
CAGTCCG855.361968E-415.2352949
Produced by FastQC (version 0.11.5)