##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633358.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	834870
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86908979841173	33.0	33.0	33.0	33.0	33.0
2	31.98405140920143	33.0	33.0	33.0	33.0	33.0
3	31.69550229377029	33.0	33.0	33.0	27.0	33.0
4	31.965859355348737	33.0	33.0	33.0	33.0	33.0
5	32.067485955897325	33.0	33.0	33.0	33.0	33.0
6	35.238680273575525	37.0	37.0	37.0	33.0	37.0
7	35.395794554840876	37.0	37.0	37.0	33.0	37.0
8	35.533043467845296	37.0	37.0	37.0	33.0	37.0
9	35.63964569334148	37.0	37.0	37.0	33.0	37.0
10	35.65499299292105	37.0	37.0	37.0	33.0	37.0
11	35.67670056415969	37.0	37.0	37.0	33.0	37.0
12	35.66717692574892	37.0	37.0	37.0	33.0	37.0
13	35.65995783774719	37.0	37.0	37.0	33.0	37.0
14	35.641823277875595	37.0	37.0	37.0	33.0	37.0
15	35.66346377280295	37.0	37.0	37.0	33.0	37.0
16	35.647664905913494	37.0	37.0	37.0	33.0	37.0
17	35.6418759806916	37.0	37.0	37.0	33.0	37.0
18	35.616149819732414	37.0	37.0	37.0	33.0	37.0
19	35.61598212895421	37.0	37.0	37.0	33.0	37.0
20	35.61399139985866	37.0	37.0	37.0	33.0	37.0
21	35.6194557236456	37.0	37.0	37.0	33.0	37.0
22	35.52543988884497	37.0	37.0	37.0	33.0	37.0
23	35.5617868650209	37.0	37.0	37.0	33.0	37.0
24	35.568472935906186	37.0	37.0	37.0	33.0	37.0
25	35.58283205768563	37.0	37.0	37.0	33.0	37.0
26	35.47993220501395	37.0	37.0	37.0	33.0	37.0
27	35.492855175057194	37.0	37.0	37.0	33.0	37.0
28	35.516428905099	37.0	37.0	37.0	33.0	37.0
29	35.517964473510844	37.0	37.0	37.0	33.0	37.0
30	35.524472073496476	37.0	37.0	37.0	33.0	37.0
31	35.519131122210645	37.0	37.0	37.0	33.0	37.0
32	35.51919580293938	37.0	37.0	37.0	33.0	37.0
33	35.50688131086277	37.0	37.0	37.0	33.0	37.0
34	35.490161342484456	37.0	37.0	37.0	33.0	37.0
35	35.43393222896978	37.0	37.0	37.0	33.0	37.0
36	35.46505324182208	37.0	37.0	37.0	33.0	37.0
37	35.461942577886376	37.0	37.0	37.0	33.0	37.0
38	35.46142513205649	37.0	37.0	37.0	33.0	37.0
39	35.36495861631152	37.0	37.0	37.0	33.0	37.0
40	35.15787487872363	37.0	37.0	37.0	33.0	37.0
41	35.3341214799909	37.0	37.0	37.0	33.0	37.0
42	35.386700923497074	37.0	37.0	37.0	33.0	37.0
43	35.04854767808162	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	15.0
16	42.0
17	30.0
18	28.0
19	25.0
20	43.0
21	138.0
22	314.0
23	819.0
24	1703.0
25	3047.0
26	5061.0
27	7696.0
28	11104.0
29	15163.0
30	19840.0
31	26143.0
32	33108.0
33	43856.0
34	63356.0
35	129395.0
36	473939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.770575059590115	19.821049983829816	13.452154227604298	24.95622072897577
2	16.398121863284103	21.198390168529233	38.467785403715546	23.935702564471114
3	18.28272665205361	26.568567561416746	29.57262807383185	25.57607771269779
4	12.874938613197266	17.47445710110556	38.785439649286715	30.86516463641046
5	14.514595086660197	36.43932588307162	34.81224621797406	14.23383281229413
6	30.413836884784455	38.922227412651075	17.14757986273312	13.516355839831352
7	27.21106280019644	31.762190520679866	23.03807778456526	17.988668894558437
8	25.05970989495371	35.36346976175931	20.236563776396324	19.340256566890655
9	26.433935822343596	15.35939727143148	19.766191143531326	38.440475762693595
10	15.439649286715296	27.424030088516776	33.35788805442764	23.778432570340293
11	34.55555954819313	23.172469965383833	23.798076347215734	18.473894139207303
12	23.453831135386345	25.02593218105813	30.13690754249165	21.38332914106388
13	28.57067567405704	21.75692023907914	25.6429144657252	24.02948962113862
14	22.557883263262543	20.493969120940985	27.446309006192582	29.501838609603894
15	24.462251608034784	28.042210164456744	24.1023153305305	23.39322289697797
16	23.532526022015404	27.790075101512812	24.73187442356295	23.94552445290884
17	23.004659408051552	26.962880448453053	26.073041311821004	23.95941883167439
18	23.962533088983914	26.42064033921449	27.041695114209396	22.5751314575922
19	25.267047564291445	25.90139782241547	26.74548133242301	22.086073280870075
20	24.826020817612324	25.677171296129934	26.782732641009975	22.714075245247763
21	23.793165402996873	26.280858097667902	26.835076119635392	23.090900379699832
22	23.474313366152813	26.093403763460177	26.829925617161955	23.602357253225055
23	23.48820774491837	26.031717512906198	27.147100746223963	23.332973995951463
24	23.646196413812927	26.399439433684286	26.447231305472705	23.50713284703008
25	23.835686993184567	26.038544923161687	27.00767784205924	23.118090241594498
26	23.6398481200666	26.358954088660507	26.922035766047408	23.079162025225482
27	23.66068968821493	26.39919987542971	26.47322337609448	23.46688706026088
28	23.392624001341524	26.148741720267825	26.960844203289135	23.497790075101513
29	24.241498676440642	26.13329021284751	26.298465629379425	23.326745481332424
30	23.932348748907014	26.0138704229401	26.86154730676632	23.192233521386562
31	23.803226849689175	25.839951130116066	26.80896426988633	23.54785775030843
32	23.08646855199013	25.967995017188304	27.008875633332135	23.936660797489427
33	23.153305305017547	26.044893216908022	27.300657587408818	23.501143890665613
34	23.688718004000624	25.751913471558446	27.00001197791273	23.5593565465282
35	23.560674116928386	26.291638219123936	26.721645286092443	23.426042377855236
36	24.124833806460884	26.14958017415885	26.54173703690395	23.183848982476313
37	23.90360175835759	25.793836166109696	26.557428102578847	23.745133972953873
38	23.76729311150239	26.21569825242253	26.452741145328012	23.564267490747064
39	23.317522488531147	26.0965180207697	26.74033082994957	23.845628660749576
40	23.497191179465066	26.43848742918059	26.83112340843485	23.233197982919496
41	23.50485704361158	25.890258363577562	26.920119300010782	23.684765292800076
42	23.34543102518955	26.47130691005785	26.81722902966929	23.366033035083305
43	23.777833674703846	25.660282439182147	26.754584546097	23.807299340017007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	34.0
2	52.0
3	167.0
4	282.0
5	282.0
6	338.5
7	395.0
8	380.0
9	365.0
10	523.5
11	682.0
12	682.0
13	1097.5
14	1513.0
15	2720.5
16	3928.0
17	4589.5
18	5251.0
19	5251.0
20	4938.0
21	4625.0
22	5073.0
23	5521.0
24	7518.0
25	9515.0
26	9515.0
27	12268.0
28	15021.0
29	18016.5
30	21012.0
31	25074.5
32	29137.0
33	29137.0
34	35026.5
35	40916.0
36	44610.0
37	48304.0
38	51492.0
39	54680.0
40	54680.0
41	57404.5
42	60129.0
43	61980.5
44	63832.0
45	63888.0
46	63944.0
47	63944.0
48	63429.5
49	62915.0
50	62327.5
51	61740.0
52	59621.5
53	57503.0
54	57503.0
55	55528.0
56	53553.0
57	48596.0
58	43639.0
59	40537.0
60	37435.0
61	37435.0
62	33221.5
63	29008.0
64	24712.0
65	20416.0
66	17292.0
67	14168.0
68	14168.0
69	12121.5
70	10075.0
71	8264.5
72	6454.0
73	5167.5
74	3881.0
75	3881.0
76	2984.5
77	2088.0
78	1639.0
79	1190.0
80	953.0
81	716.0
82	716.0
83	577.0
84	438.0
85	360.5
86	283.0
87	218.0
88	153.0
89	153.0
90	111.5
91	70.0
92	45.0
93	20.0
94	12.0
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	834870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.07113393808874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88343978040217	54.38578239295836
2	8.953977034854425	11.473829237574568
3	2.510455415669696	4.825431755489198
4	1.0925509163547538	2.8000390438378804
5	0.6227477383179284	1.995007687570491
6	0.38639061745297704	1.4853891001950297
7	0.27142803983619485	1.2173491606428406
8	0.1917026842994094	0.9826086689630877
9	0.141911856334621	0.8183208199156475
>10	0.8254750672780484	9.928196132548768
>50	0.07044307333675723	3.121817910497246
>100	0.04740046467335997	5.630794400337991
>500	0.0015107717743247666	0.5466602708181938
>1k	5.665394153717875E-4	0.7887734186506873
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3176	0.38041850827074875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2135	0.25572843676260976	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1208	0.1446931857654485	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.1977912728927857E-4	0.0
3	0.0	0.0	2.3955825457855714E-4	1.1977912728927857E-4	0.0
4	0.0	0.0	4.791165091571143E-4	2.3955825457855714E-4	0.0
5	0.0	0.0	5.988956364463929E-4	2.3955825457855714E-4	0.0
6	0.0	0.0	0.0011977912728927858	2.3955825457855714E-4	0.0
7	0.0	0.0	0.0026351408003641285	3.593373818678357E-4	0.0
8	0.0	0.0	0.003114257309521243	3.593373818678357E-4	0.0
9	1.1977912728927857E-4	0.0	0.00419226945512475	7.186747637356714E-4	0.0
10	1.1977912728927857E-4	0.0	0.004791165091571143	8.3845389102495E-4	0.0
11	1.1977912728927857E-4	0.0	0.006827410255488878	8.3845389102495E-4	0.0
12	1.1977912728927857E-4	0.0	0.006827410255488878	9.582330183142286E-4	0.0
13	1.1977912728927857E-4	0.0	0.0074263058919352715	9.582330183142286E-4	0.0
14	1.1977912728927857E-4	0.0	0.008025201528381664	9.582330183142286E-4	0.0
15	1.1977912728927857E-4	0.0	0.008504318037538778	9.582330183142286E-4	0.0
16	1.1977912728927857E-4	0.0	0.009103213673985172	9.582330183142286E-4	0.0
17	1.1977912728927857E-4	0.0	0.009342771928563729	0.0011977912728927858	0.0
18	1.1977912728927857E-4	0.0	0.009462551055853008	0.0011977912728927858	0.0
19	1.1977912728927857E-4	0.0	0.009582330183142286	0.0011977912728927858	0.0
20	1.1977912728927857E-4	0.0	0.009702109310431565	0.0013175704001820642	0.0
21	1.1977912728927857E-4	0.0	0.009941667565010122	0.0015571286547606214	0.0
22	1.1977912728927857E-4	0.0	0.010181225819588679	0.0017966869093391786	0.0
23	1.1977912728927857E-4	0.0	0.010420784074167235	0.002156024291207014	0.0
24	1.1977912728927857E-4	0.0	0.010540563201456514	0.002754919927653407	0.0
25	1.1977912728927857E-4	0.0	0.010540563201456514	0.0029944781822319644	0.0
26	1.1977912728927857E-4	0.0	0.010540563201456514	0.0032340364368105214	0.0
27	1.1977912728927857E-4	0.0	0.010540563201456514	0.00419226945512475	0.0
28	1.1977912728927857E-4	0.0	0.010540563201456514	0.013175704001820642	0.0
29	1.1977912728927857E-4	0.0	0.010540563201456514	0.030304119204187477	0.0
30	1.1977912728927857E-4	0.0	0.010540563201456514	0.052223699498125456	0.0
31	1.1977912728927857E-4	0.0	0.010540563201456514	0.100614466922994	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	735	0.0	24.666666	1
GTATCAA	1860	0.0	23.274195	1
CGCCGCT	170	5.456968E-12	17.411764	26
CCGCTCT	140	1.8662831E-9	17.178572	28
GGTCGCC	175	7.2759576E-12	16.914286	36
AGGTCGC	165	5.4569682E-11	16.818182	35
GCCGCTC	155	4.0017767E-10	16.709679	27
TTCGCCG	155	4.0017767E-10	16.709679	24
TATCAAC	2665	0.0	16.035646	2
ATCAACG	2675	0.0	15.975701	3
TCAACGC	2715	0.0	15.944752	4
CCTACCG	105	9.336898E-6	15.857142	24
AACGGCC	140	3.468631E-8	15.857142	37
GTAGTAT	105	9.336898E-6	15.857142	3
CAACGCA	2755	0.0	15.713249	5
AACGCAG	2790	0.0	15.516129	6
GCGACAA	120	1.9338586E-6	15.416667	16
GTCGCCC	180	2.0190782E-10	15.416666	37
TGCTCGG	205	5.456968E-12	15.341464	10
CAGTCCG	85	5.361968E-4	15.235294	9
>>END_MODULE