FastQCFastQC Report
Fri 10 Feb 2017
ERR1633357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611613
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40230.6577688832644172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29100.4757910639571101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16590.271249957080703No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8580.14028478792962215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.1196835253665308No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6300.10300631281545683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG502.717261E-729.59999835
AACGGCC509.08201E-625.89999837
GGTATCA7850.024.9808921
CTGTCGC400.001930159923.1259
AGGTCCG400.001930159923.1258
GTATCAA19550.022.7109991
AGCGATA502.7000997E-422.19999911
TTCGCCG855.1706593E-821.76470624
GCCGGCA957.1286195E-921.42105315
GCCGCTC909.460018E-820.55555727
TTAGCGA555.139312E-420.1818189
CCGTGTA555.139312E-420.18181833
TCGCTAC555.139312E-420.18181827
TTCGCTA555.139312E-420.18181826
GCTTAAG656.895382E-519.9230771
GAGTCGC609.2300813E-418.522
GTACTAG609.2300813E-418.51
CCGACAA701.2180136E-418.514
ACCCGAC609.2300813E-418.512
ATTTCGC609.2300813E-418.524
Produced by FastQC (version 0.11.5)