FastQCFastQC Report
Fri 10 Feb 2017
ERR1633356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958812
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62050.6471550210051605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43390.45253918390675124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23210.24207039544769984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.11910572667008758No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10750.11211791258348873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.024.7172131
GTATCAA32150.022.2690531
AGCAACG450.003825351620.5555572
ATCTCGT450.003825351620.55555737
GCAACGC450.003825351620.5555573
GACCCGA450.003825351620.5555576
GCCGGCA1457.2759576E-1219.1379315
CGAGCCC609.235291E-418.515
TTCGCCG1451.546141E-1017.86206824
GACCGTG953.6051242E-617.5263167
AGCGCAG752.0668161E-417.26666637
CGAGTCG1301.3922545E-817.07692321
CCGTACA2400.016.95833417
GAGTCGC1352.2177119E-816.44444522
TAGGACC2150.016.3488374
GACCGTT803.3818328E-416.18757
TAAACGA803.3818328E-416.187529
TCGCAAG700.002592072615.85714319
GCCGCTC1659.731593E-1015.6969727
GGACCGT1302.5874579E-715.6538466
Produced by FastQC (version 0.11.5)