##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	958812
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.918280121650543	33.0	33.0	33.0	33.0	33.0
2	32.024935023758566	33.0	33.0	33.0	33.0	33.0
3	31.74323016399461	33.0	33.0	33.0	27.0	33.0
4	32.0111346124162	33.0	33.0	33.0	33.0	33.0
5	32.114909909346146	33.0	33.0	33.0	33.0	33.0
6	35.27980354855801	37.0	37.0	37.0	33.0	37.0
7	35.41846889692661	37.0	37.0	37.0	33.0	37.0
8	35.54883439089206	37.0	37.0	37.0	33.0	37.0
9	35.65787349344814	37.0	37.0	37.0	33.0	37.0
10	35.659499463919936	37.0	37.0	37.0	33.0	37.0
11	35.691780036128044	37.0	37.0	37.0	33.0	37.0
12	35.68208991960885	37.0	37.0	37.0	33.0	37.0
13	35.685624501987874	37.0	37.0	37.0	33.0	37.0
14	35.65066040057905	37.0	37.0	37.0	33.0	37.0
15	35.6748309366174	37.0	37.0	37.0	33.0	37.0
16	35.66787128237861	37.0	37.0	37.0	33.0	37.0
17	35.65527757266284	37.0	37.0	37.0	33.0	37.0
18	35.637997855679735	37.0	37.0	37.0	33.0	37.0
19	35.62618219212943	37.0	37.0	37.0	33.0	37.0
20	35.624238119673095	37.0	37.0	37.0	33.0	37.0
21	35.625440649470384	37.0	37.0	37.0	33.0	37.0
22	35.531839401259056	37.0	37.0	37.0	33.0	37.0
23	35.584418008952746	37.0	37.0	37.0	33.0	37.0
24	35.576977551386506	37.0	37.0	37.0	33.0	37.0
25	35.603238173906874	37.0	37.0	37.0	33.0	37.0
26	35.4840458817787	37.0	37.0	37.0	33.0	37.0
27	35.51036595286667	37.0	37.0	37.0	33.0	37.0
28	35.52844040333246	37.0	37.0	37.0	33.0	37.0
29	35.525920618431975	37.0	37.0	37.0	33.0	37.0
30	35.53233689190373	37.0	37.0	37.0	33.0	37.0
31	35.52012386161208	37.0	37.0	37.0	33.0	37.0
32	35.5064538199355	37.0	37.0	37.0	33.0	37.0
33	35.49951919667255	37.0	37.0	37.0	33.0	37.0
34	35.476232045489624	37.0	37.0	37.0	33.0	37.0
35	35.4115238440904	37.0	37.0	37.0	33.0	37.0
36	35.43357404788426	37.0	37.0	37.0	33.0	37.0
37	35.43135359173644	37.0	37.0	37.0	33.0	37.0
38	35.42013658569146	37.0	37.0	37.0	33.0	37.0
39	35.31411162980856	37.0	37.0	37.0	33.0	37.0
40	35.09425205358298	37.0	37.0	37.0	33.0	37.0
41	35.26143811299817	37.0	37.0	37.0	33.0	37.0
42	35.30565741772109	37.0	37.0	37.0	33.0	37.0
43	34.96594327146511	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	22.0
16	53.0
17	43.0
18	33.0
19	38.0
20	57.0
21	158.0
22	424.0
23	958.0
24	2069.0
25	3694.0
26	5971.0
27	9029.0
28	12759.0
29	17702.0
30	22934.0
31	29427.0
32	37600.0
33	49302.0
34	71505.0
35	146428.0
36	548604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92511149213819	20.412135017083642	14.18432393420191	23.478429556576263
2	15.563113519647231	22.548945987325983	39.88988456548312	21.998055927543668
3	17.84385260092698	27.943434166447645	30.9984647668156	23.214248465809774
4	12.269662874473829	18.127745585161637	40.2268640776294	29.375727462735135
5	13.41086678097479	37.55324297151058	35.16184611790424	13.874044129610393
6	28.168608653208345	40.60191153218775	17.738931093895363	13.490548720708542
7	25.261782288915867	33.22058964635403	23.75835930297076	17.759268761759344
8	24.092522830335874	35.68217752802426	21.168696261623758	19.056603380016103
9	26.2624998435564	15.639979474599816	20.524565816865035	37.57295486497875
10	14.530794358018047	28.511845909312772	34.09125042239772	22.866109310271458
11	32.733424279212194	24.496043019903798	24.53442384951377	18.236108851370236
12	22.728126055994295	26.296813139593578	30.89260459819026	20.082456206221867
13	28.171633229454784	22.945791250005215	26.703045018210037	22.179530502329968
14	22.100787224189936	21.90283392364718	28.82223000963692	27.17414884252596
15	23.586479935586954	28.939249821654293	25.704204786756947	21.770065456001802
16	21.943509259375144	29.323266709219325	25.69012486285111	23.04309916855442
17	21.924318844570156	27.715756582103683	26.94553259658828	23.414391976737882
18	22.848691922921283	26.895053461992553	28.552312653575466	21.703941961510704
19	24.52806180982299	26.395164015469142	28.85247577210131	20.224298402606557
20	24.44577247677334	26.037742539726246	28.619687696858193	20.89679728664222
21	22.72395422668886	27.33518145371564	27.970238169735044	21.970626149860454
22	21.950288481996473	27.437286975966092	28.361242871386676	22.251181670650762
23	22.005043741630267	27.003312432468512	28.84600943667789	22.145634389223332
24	22.610167582383202	27.123565412197593	28.347475834678747	21.91879117074046
25	22.580651890047267	26.968373362035518	28.703019987234203	21.74795476068301
26	22.509313608924376	26.97807286517065	28.32640809668632	22.18620542921866
27	22.431091809447526	27.013846301464728	28.336420487019353	22.218641402068393
28	21.742844269783856	27.253726486527075	28.655878316082816	22.347550927606246
29	22.667634531065527	27.202100098872357	28.094558683036926	22.035706687025193
30	22.305832634551926	27.481612662336307	28.370629487323896	21.84192521578787
31	22.695794378877192	26.9901711701564	28.3636416732373	21.950392777729107
32	21.465521916705256	27.04367488099857	28.7169956154074	22.773807586888775
33	21.747537577752468	26.85875854703529	29.23336378768726	22.16034008752498
34	21.833685852909642	26.798684205037066	28.643571419631797	22.724058522421497
35	22.086185821620923	27.52750278469606	28.426010521353508	21.96030087232951
36	22.402201891507406	27.17237581507115	28.349770340796738	22.075651952624707
37	22.871428392635888	26.793260826940006	27.92716403215646	22.408146748267647
38	22.694334238620293	27.188958836560246	28.09424579583902	22.022461128980446
39	22.335556918353127	26.84759890364326	28.38742110027826	22.429423077725353
40	22.26786898787249	27.200118479952273	28.511950205045412	22.02006232712982
41	22.170769660788558	26.806193497786847	28.65566972461755	22.36736711680705
42	22.13019862079323	26.997054688510364	28.34372118830386	22.529025502392543
43	21.990650930526527	26.802960330075138	28.522796961239532	22.683591778158803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	37.0
1	65.5
2	94.0
3	347.0
4	600.0
5	600.0
6	724.0
7	848.0
8	836.5
9	825.0
10	1138.5
11	1452.0
12	1452.0
13	2291.0
14	3130.0
15	5821.0
16	8512.0
17	9747.5
18	10983.0
19	10983.0
20	10337.5
21	9692.0
22	10770.5
23	11849.0
24	15025.5
25	18202.0
26	18202.0
27	22119.5
28	26037.0
29	32022.5
30	38008.0
31	41142.0
32	44276.0
33	44276.0
34	48353.0
35	52430.0
36	55291.5
37	58153.0
38	60998.0
39	63843.0
40	63843.0
41	66236.0
42	68629.0
43	66567.0
44	64505.0
45	65994.5
46	67484.0
47	67484.0
48	66890.0
49	66296.0
50	67694.0
51	69092.0
52	69153.0
53	69214.0
54	69214.0
55	62452.5
56	55691.0
57	50032.5
58	44374.0
59	38948.5
60	33523.0
61	33523.0
62	29066.5
63	24610.0
64	20245.5
65	15881.0
66	13449.5
67	11018.0
68	11018.0
69	9034.0
70	7050.0
71	6114.5
72	5179.0
73	4154.0
74	3129.0
75	3129.0
76	2298.0
77	1467.0
78	1192.5
79	918.0
80	736.5
81	555.0
82	555.0
83	490.0
84	425.0
85	403.0
86	381.0
87	312.0
88	243.0
89	243.0
90	188.0
91	133.0
92	84.0
93	35.0
94	22.0
95	9.0
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	958812.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.38211211873743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.7530703739195	53.86468804455382
2	7.701390227243872	9.454551967976641
3	1.7054874992873608	3.140592746950857
4	0.7151163936703155	1.7558141861687395
5	0.40607316692358236	1.2462814330257053
6	0.26997543228421417	0.9942997352264544
7	0.18334027279480855	0.7877669226399591
8	0.14000751904197525	0.6875165785040632
9	0.10112136607343179	0.5586338726927353
>10	0.8143871419777333	10.314117534069572
>50	0.12355175584008556	5.2932401650141605
>100	0.08253239549960115	9.078273736607251
>500	0.0030885303471023763	1.2357489971059596
>1k	6.863400771338614E-4	0.9349479116431947
>5k	1.7158501928346535E-4	0.653526167820888
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6205	0.6471550210051605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4339	0.45253918390675124	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.24207039544769984	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1142	0.11910572667008758	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1075	0.11211791258348873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0859146527160694E-4	1.0429573263580347E-4	0.0
2	0.0	0.0	3.1288719790741043E-4	1.0429573263580347E-4	0.0
3	0.0	0.0	4.1718293054321387E-4	1.0429573263580347E-4	0.0
4	0.0	0.0	5.214786631790174E-4	2.0859146527160694E-4	0.0
5	0.0	0.0	8.343658610864277E-4	3.1288719790741043E-4	0.0
6	0.0	0.0	0.001773027454808659	3.1288719790741043E-4	0.0
7	0.0	0.0	0.0034417591769815145	4.1718293054321387E-4	0.0
8	0.0	0.0	0.00479760370124696	4.1718293054321387E-4	0.0
9	0.0	0.0	0.005631969562333388	0.0011472530589938382	0.0
10	2.0859146527160694E-4	0.0	0.0065706311560556185	0.0011472530589938382	0.0
11	2.0859146527160694E-4	0.0	0.010116686065672937	0.0012515487916296417	0.0
12	2.0859146527160694E-4	0.0	0.011055347659395169	0.0012515487916296417	0.0
13	2.0859146527160694E-4	0.0	0.011576826322574186	0.0012515487916296417	0.0
14	2.0859146527160694E-4	0.0	0.012724079381568023	0.0012515487916296417	0.0
15	2.0859146527160694E-4	0.0	0.014184219638469272	0.0012515487916296417	0.0
16	2.0859146527160694E-4	0.0	0.0150185854995557	0.0012515487916296417	0.0
17	2.0859146527160694E-4	0.0	0.015435768430098914	0.0013558445242654452	0.0
18	2.0859146527160694E-4	0.0	0.01616583855854954	0.001564435989537052	0.0
19	2.0859146527160694E-4	0.0	0.01647872575645695	0.0016687317221728555	0.0
20	2.0859146527160694E-4	0.0	0.016895908687000163	0.001773027454808659	0.0
21	2.0859146527160694E-4	0.0	0.01741738735017918	0.002190210385351873	0.0
22	2.0859146527160694E-4	0.0	0.01773027454808659	0.00239880185062348	0.0
23	2.0859146527160694E-4	0.0	0.018043161745994	0.0032331677117099075	0.0
24	2.0859146527160694E-4	0.0	0.01835604894390141	0.0040675335727963354	0.0
25	3.1288719790741043E-4	0.0	0.01835604894390141	0.004380420770703746	0.0
26	3.1288719790741043E-4	0.0	0.01856464040917302	0.005840561027604995	0.0
27	3.1288719790741043E-4	0.0	0.01856464040917302	0.007300701284506243	0.0
28	3.1288719790741043E-4	0.0	0.01856464040917302	0.01533147269746311	0.0
29	3.1288719790741043E-4	0.0	0.01856464040917302	0.03629491495725961	0.0
30	3.1288719790741043E-4	0.0	0.01856464040917302	0.06487194569946976	0.0
31	3.1288719790741043E-4	0.0	0.01856464040917302	0.1319341017842914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1220	0.0	24.717213	1
GTATCAA	3215	0.0	22.269053	1
AGCAACG	45	0.0038253516	20.555557	2
ATCTCGT	45	0.0038253516	20.555557	37
GCAACGC	45	0.0038253516	20.555557	3
GACCCGA	45	0.0038253516	20.555557	6
GCCGGCA	145	7.2759576E-12	19.13793	15
CGAGCCC	60	9.235291E-4	18.5	15
TTCGCCG	145	1.546141E-10	17.862068	24
GACCGTG	95	3.6051242E-6	17.526316	7
AGCGCAG	75	2.0668161E-4	17.266666	37
CGAGTCG	130	1.3922545E-8	17.076923	21
CCGTACA	240	0.0	16.958334	17
GAGTCGC	135	2.2177119E-8	16.444445	22
TAGGACC	215	0.0	16.348837	4
GACCGTT	80	3.3818328E-4	16.1875	7
TAAACGA	80	3.3818328E-4	16.1875	29
TCGCAAG	70	0.0025920726	15.857143	19
GCCGCTC	165	9.731593E-10	15.69697	27
GGACCGT	130	2.5874579E-7	15.653846	6
>>END_MODULE