FastQCFastQC Report
Fri 10 Feb 2017
ERR1633355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807958
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34800.4307154579817268No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.30595649773874384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14240.17624678510516636No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11900.14728488362018818No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC10600.13119493835075585No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC10520.13020478787263695No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9630.11918936380356404No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8810.10904032140284521No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8290.10260434329507227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.027.8641971
GTATCAA20900.021.9521521
TTCTGCG705.0988274E-621.14285918
TCGCCAT1600.020.812513
TTCGCAC450.00382483920.55555527
GCCGGCA1303.274181E-1119.92307715
GTCGCCA1700.019.58823612
AGAGGTC2150.018.9302338
TCGCACC500.00703311718.528
CGAGTCG801.6157019E-518.521
AGCTTCG1552.0008883E-1117.90322721
TGCTCGC1552.0008883E-1117.90322710
CGCTCTC1451.546141E-1017.86206829
CCGCTCT1351.1496013E-917.81481628
TTCGCCG1351.1496013E-917.81481624
TGCCCGT1258.56744E-917.7623
GGCCGCA1900.017.52631633
CGTCAAT852.7214945E-517.41176430
CCTAGTA752.0663087E-417.2666662
CCGTTAA650.001579384817.07692316
Produced by FastQC (version 0.11.5)