FastQCFastQC Report
Fri 10 Feb 2017
ERR1633354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579292
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24250.4186144466003328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.30071190349599164No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC12210.21077453166969337No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10680.18436298101820842No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC10020.1729697630901169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9600.1657195334995132No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9590.16554690898545119No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC9510.16416591287295526No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9430.1627849167604593No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG8300.14327834667145412No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG7740.1336113738839825No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA7200.1242896501246349No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT6800.11738466956215518No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG6710.11583104893559724No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6670.11514055087934927No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG6420.11082493802779946No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6420.11082493802779946No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC6230.10754507226062159No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT6070.1047830800356297No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT6020.10391995746531973No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT5970.10305683489500977No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC5910.10202108781063782No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG5880.10150321426845184No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT5810.1002948426700179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG358.864064E-426.4285721
CGACGTT358.864064E-426.4285714
GCCGGCA509.080926E-625.90000215
CGCCGTC400.001930036923.12500226
GGTATCA5100.022.127451
TTCGCCG603.7221806E-521.58333224
GTATCAA12300.020.75611
TTACGGC450.003823552320.55555518
TGACTCG450.003823552320.55555510
TTACTCG555.138861E-420.18181819
GTCCGGC555.138861E-420.18181815
TATTAGC1202.382876E-1020.0416662
TCGCCGG656.894578E-519.92307713
AGCTTCG656.894578E-519.92307721
TTAACGG2850.018.8245635
TCGCCGT500.00703077318.525
CGAACCG1103.8417056E-818.531
TAGACGC500.00703077318.514
TGGACGT500.00703077318.526
CCGGAAT500.00703077318.53
Produced by FastQC (version 0.11.5)