##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633354.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	579292
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8694561637309	33.0	33.0	33.0	33.0	33.0
2	31.98905387956333	33.0	33.0	33.0	33.0	33.0
3	31.695764830172003	33.0	33.0	33.0	27.0	33.0
4	31.970634843912915	33.0	33.0	33.0	33.0	33.0
5	32.07495183776058	33.0	33.0	33.0	33.0	33.0
6	35.24801827057857	37.0	37.0	37.0	33.0	37.0
7	35.39605587510271	37.0	37.0	37.0	33.0	37.0
8	35.51919929845398	37.0	37.0	37.0	33.0	37.0
9	35.63708457910691	37.0	37.0	37.0	33.0	37.0
10	35.629748037259276	37.0	37.0	37.0	33.0	37.0
11	35.65651346816459	37.0	37.0	37.0	33.0	37.0
12	35.64898876559663	37.0	37.0	37.0	33.0	37.0
13	35.6549926461957	37.0	37.0	37.0	33.0	37.0
14	35.62666841592841	37.0	37.0	37.0	33.0	37.0
15	35.641738536006024	37.0	37.0	37.0	33.0	37.0
16	35.640628560380605	37.0	37.0	37.0	33.0	37.0
17	35.630828666717306	37.0	37.0	37.0	33.0	37.0
18	35.614954116404164	37.0	37.0	37.0	33.0	37.0
19	35.60441711606582	37.0	37.0	37.0	33.0	37.0
20	35.609200541350475	37.0	37.0	37.0	33.0	37.0
21	35.60366447318451	37.0	37.0	37.0	33.0	37.0
22	35.50252031790531	37.0	37.0	37.0	33.0	37.0
23	35.56032191019382	37.0	37.0	37.0	33.0	37.0
24	35.54955531925177	37.0	37.0	37.0	33.0	37.0
25	35.564628891819666	37.0	37.0	37.0	33.0	37.0
26	35.4699288096504	37.0	37.0	37.0	33.0	37.0
27	35.48056765845204	37.0	37.0	37.0	33.0	37.0
28	35.50466776686024	37.0	37.0	37.0	33.0	37.0
29	35.50817894947626	37.0	37.0	37.0	33.0	37.0
30	35.504015246197085	37.0	37.0	37.0	33.0	37.0
31	35.499195569764474	37.0	37.0	37.0	33.0	37.0
32	35.48751579514304	37.0	37.0	37.0	33.0	37.0
33	35.49524419463759	37.0	37.0	37.0	33.0	37.0
34	35.462600899028466	37.0	37.0	37.0	33.0	37.0
35	35.41046829578175	37.0	37.0	37.0	33.0	37.0
36	35.43946576165388	37.0	37.0	37.0	33.0	37.0
37	35.43336866381721	37.0	37.0	37.0	33.0	37.0
38	35.43014058540425	37.0	37.0	37.0	33.0	37.0
39	35.33763801329899	37.0	37.0	37.0	33.0	37.0
40	35.13770602735753	37.0	37.0	37.0	33.0	37.0
41	35.29592675196619	37.0	37.0	37.0	33.0	37.0
42	35.34274597267009	37.0	37.0	37.0	33.0	37.0
43	35.01469207239182	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	14.0
16	29.0
17	27.0
18	17.0
19	22.0
20	33.0
21	74.0
22	268.0
23	619.0
24	1240.0
25	2140.0
26	3707.0
27	5506.0
28	7798.0
29	10605.0
30	13731.0
31	18228.0
32	22828.0
33	30442.0
34	44393.0
35	90497.0
36	327072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07324112882622	21.000635258211748	13.464366847807325	24.461756765154707
2	16.28418828500998	21.196046208129925	39.136566705564725	23.383198801295375
3	17.318036499727256	27.580391236198672	29.87560677516693	25.22596548890715
4	12.175034352278297	17.921186551859858	40.20994593400219	29.69383316185965
5	13.454527250505791	37.59261305179426	35.83408712704474	13.118772570655214
6	29.470802289691555	40.309550278615966	17.236730353604056	12.982917078088423
7	26.99899187283788	32.00493015612161	23.6937503020929	17.302327668947612
8	24.368884776589354	36.14532912589851	20.396276834480712	19.089509263031424
9	25.81323408574605	15.525503545707519	20.44392120036182	38.21734116818461
10	15.213225799769372	28.00211292405212	34.01376162626102	22.770899649917485
11	33.670756716819845	23.973919888415516	24.029504981943475	18.325818412821167
12	23.210919536261507	26.17263832402312	29.784461031742193	20.83198110797318
13	26.931150438811514	22.522320349668217	26.464201128273825	24.082328083246445
14	21.85909696664204	21.13252038695511	27.837083888608856	29.171298757793995
15	23.890714872637634	28.817591128480974	25.02175068877181	22.269943310109582
16	23.08990975190405	28.556237614191115	25.006559731534356	23.34729290237048
17	22.375244263687396	27.756295616027842	26.22304468212922	23.645415438155542
18	22.42047188637164	27.605421790737662	28.259150825490426	21.714955497400275
19	24.902294525040915	26.21890169379173	27.386706531421112	21.49209724974624
20	23.64783218135241	26.72814401027461	27.957403174910063	21.666620633462916
21	22.849098554787567	27.29107255063077	27.674126347334333	22.18570254724733
22	22.598793009397678	27.077536026736084	27.582635354881475	22.74103560898476
23	22.481580964349586	27.09272698397354	28.208053969328077	22.2176380823488
24	23.03898552025576	27.965516527070978	26.109803000904552	22.885694951768713
25	22.69856997852551	27.379283677316447	27.91718166313362	22.004964681024422
26	23.135827872644537	27.346830268672797	27.804285230937076	21.71305662774559
27	22.907618265054584	27.26586937157772	26.533768807440804	23.29274355592689
28	21.977344758774503	27.002271738605053	28.45611539603516	22.56426810658528
29	24.158110244919662	26.877636839452297	27.134847365404667	21.829405550223377
30	23.35799562224232	26.528762696533008	27.79720762586053	22.316034055364135
31	22.56772059686652	26.932186185895883	27.9277117584914	22.572381458746193
32	21.746891032501743	26.578823805610984	28.141248282386083	23.533036879501186
33	21.825607810914015	26.84690967594926	28.55140412779738	22.776078385339346
34	23.167245534203822	26.751620944187042	28.238090634774863	21.843042886834276
35	22.99548414271214	27.480959516098963	27.147448954931193	22.376107386257708
36	23.775229072730163	27.192676577615433	26.617491696760876	22.41460265289353
37	22.675265669127143	26.478528962940967	27.484584630894265	23.361620737037626
38	23.12650614888519	26.837070078647727	27.252404659480884	22.784019112986197
39	22.184321551134833	27.08910186917824	27.69639490964833	23.030181670038598
40	22.809049667525187	27.52049052981916	27.895085725333686	21.77537407732197
41	22.332260759685962	26.352858316703838	27.970177388950653	23.34470353465955
42	21.84373338489052	27.91303867479613	27.45299434482092	22.790233595492428
43	23.313803746642453	25.77007795723055	27.656863895928137	23.259254400198863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	39.0
2	43.0
3	147.5
4	252.0
5	252.0
6	289.5
7	327.0
8	328.0
9	329.0
10	413.0
11	497.0
12	497.0
13	887.0
14	1277.0
15	2251.5
16	3226.0
17	3742.5
18	4259.0
19	4259.0
20	4100.0
21	3941.0
22	4402.0
23	4863.0
24	6328.0
25	7793.0
26	7793.0
27	9937.0
28	12081.0
29	14245.0
30	16409.0
31	19681.0
32	22953.0
33	22953.0
34	28643.5
35	34334.0
36	37404.0
37	40474.0
38	42500.5
39	44527.0
40	44527.0
41	45782.0
42	47037.0
43	47806.0
44	48575.0
45	46033.0
46	43491.0
47	43491.0
48	40870.5
49	38250.0
50	38492.0
51	38734.0
52	37573.5
53	36413.0
54	36413.0
55	36307.0
56	36201.0
57	30363.5
58	24526.0
59	22206.5
60	19887.0
61	19887.0
62	18295.0
63	16703.0
64	13180.5
65	9658.0
66	7934.0
67	6210.0
68	6210.0
69	5257.0
70	4304.0
71	3970.0
72	3636.0
73	3787.0
74	3938.0
75	3938.0
76	3144.0
77	2350.0
78	1716.0
79	1082.0
80	684.0
81	286.0
82	286.0
83	231.0
84	176.0
85	145.5
86	115.0
87	94.0
88	73.0
89	73.0
90	48.5
91	24.0
92	13.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	579292.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.140081438304556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.74612436639029	46.62836194725579
2	6.709160611146156	7.130506825179437
3	1.7248983121696335	2.749837103444652
4	0.8096146338841481	1.7209195031298703
5	0.49932850749735397	1.3267178776438227
6	0.35152382091134016	1.120800268243956
7	0.23237923693629486	0.8644056102756044
8	0.1864262985409996	0.7925366949368317
9	0.17713301085363473	0.847157635995681
>10	1.2431489120499228	13.430939241086598
>50	0.18192218747883993	6.741185120767061
>100	0.12810491558682738	12.284740473887227
>500	0.008584350013550289	3.053376824060376
>1k	0.0016508365410673631	1.3085148740931039
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2425	0.4186144466003328	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1742	0.30071190349599164	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1221	0.21077453166969337	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1068	0.18436298101820842	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1002	0.1729697630901169	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	960	0.1657195334995132	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	959	0.16554690898545119	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	951	0.16416591287295526	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	943	0.1627849167604593	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	830	0.14327834667145412	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	774	0.1336113738839825	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA	720	0.1242896501246349	No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACAT	680	0.11738466956215518	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	671	0.11583104893559724	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	667	0.11514055087934927	No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCG	642	0.11082493802779946	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	642	0.11082493802779946	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	623	0.10754507226062159	No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCAT	607	0.1047830800356297	No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACAT	602	0.10391995746531973	No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT	597	0.10305683489500977	No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC	591	0.10202108781063782	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	588	0.10150321426845184	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	581	0.1002948426700179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	1.7262451406199292E-4	0.0	0.0
4	0.0	0.0	3.4524902812398583E-4	1.7262451406199292E-4	0.0
5	0.0	0.0	3.4524902812398583E-4	1.7262451406199292E-4	0.0
6	0.0	0.0	8.631225703099645E-4	1.7262451406199292E-4	0.0
7	0.0	0.0	0.0015536206265579363	3.4524902812398583E-4	0.0
8	0.0	0.0	0.001898869654681922	5.178735421859787E-4	0.0
9	0.0	0.0	0.002589367710929894	0.0010357470843719574	0.0
10	0.0	0.0	0.0027619922249918867	0.0012083715984339504	0.0
11	0.0	0.0	0.004488237365611816	0.0012083715984339504	0.0
12	0.0	0.0	0.0048334863937358015	0.0012083715984339504	0.0
13	0.0	0.0	0.005523984449983773	0.0012083715984339504	0.0
14	0.0	0.0	0.0056966089640457665	0.0013809961124959433	0.0
15	0.0	0.0	0.0063871070202937376	0.0013809961124959433	0.0
16	0.0	0.0	0.0070776050765417094	0.0015536206265579363	1.7262451406199292E-4
17	0.0	0.0	0.007250229590603703	0.0015536206265579363	1.7262451406199292E-4
18	0.0	0.0	0.007250229590603703	0.001726245140619929	1.7262451406199292E-4
19	0.0	0.0	0.007422854104665695	0.001726245140619929	1.7262451406199292E-4
20	0.0	0.0	0.007422854104665695	0.001898869654681922	1.7262451406199292E-4
21	0.0	0.0	0.007422854104665695	0.002071494168743915	1.7262451406199292E-4
22	0.0	0.0	0.007595478618727688	0.0024167431968679007	1.7262451406199292E-4
23	0.0	0.0	0.007595478618727688	0.0027619922249918867	1.7262451406199292E-4
24	0.0	0.0	0.007595478618727688	0.0032798657671778654	1.7262451406199292E-4
25	0.0	0.0	0.007595478618727688	0.0032798657671778654	1.7262451406199292E-4
26	0.0	0.0	0.007595478618727688	0.003797739309363844	1.7262451406199292E-4
27	0.0	0.0	0.007595478618727688	0.004488237365611816	1.7262451406199292E-4
28	0.0	0.0	0.007595478618727688	0.013119463068711461	1.7262451406199292E-4
29	0.0	0.0	0.007595478618727688	0.02606630162336093	1.7262451406199292E-4
30	0.0	0.0	0.007595478618727688	0.045400247198304135	1.7262451406199292E-4
31	0.0	0.0	0.007595478618727688	0.09580660530440607	1.7262451406199292E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCG	35	8.864064E-4	26.42857	21
CGACGTT	35	8.864064E-4	26.42857	14
GCCGGCA	50	9.080926E-6	25.900002	15
CGCCGTC	40	0.0019300369	23.125002	26
GGTATCA	510	0.0	22.12745	1
TTCGCCG	60	3.7221806E-5	21.583332	24
GTATCAA	1230	0.0	20.7561	1
TTACGGC	45	0.0038235523	20.555555	18
TGACTCG	45	0.0038235523	20.555555	10
TTACTCG	55	5.138861E-4	20.181818	19
GTCCGGC	55	5.138861E-4	20.181818	15
TATTAGC	120	2.382876E-10	20.041666	2
TCGCCGG	65	6.894578E-5	19.923077	13
AGCTTCG	65	6.894578E-5	19.923077	21
TTAACGG	285	0.0	18.82456	35
TCGCCGT	50	0.007030773	18.5	25
CGAACCG	110	3.8417056E-8	18.5	31
TAGACGC	50	0.007030773	18.5	14
TGGACGT	50	0.007030773	18.5	26
CCGGAAT	50	0.007030773	18.5	3
>>END_MODULE