FastQCFastQC Report
Fri 10 Feb 2017
ERR1633353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762892
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45860.601133581162209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.4311226228614273No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17940.23515779428805125No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8010.10499520246640415No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.1030290001730258No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC7650.10027631696229611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGT358.8666484E-426.42857229
ATCCGTC358.8666484E-426.42857230
GGTATCA6700.025.4029851
GTATCAA20350.024.4545461
CCGTCCG957.1358954E-921.42105328
ACGGTAC909.46784E-820.5555553
CGCCGTC1052.2564564E-819.38095326
TCGCCAT1457.2759576E-1219.1379313
CGGCCTT1306.9485395E-1018.524
GCCGGCA1306.9485395E-1018.515
ACTGTAT801.615509E-518.58
CGTCCGC1103.846617E-818.529
CGTCCTA500.007032768318.49999833
TGTACCG500.007032768318.4999985
CGGTACT1054.7920366E-717.619054
TCACGGT953.6031706E-617.5263161
TGTCCGG1502.5102054E-1017.26666614
CGAGTCG1401.8662831E-917.17857221
CCATCGG650.001579275617.07692319
TCGCCGT1201.0398071E-716.95833425
Produced by FastQC (version 0.11.5)