##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762892
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.911060805461325	33.0	33.0	33.0	33.0	33.0
2	32.014175007733726	33.0	33.0	33.0	33.0	33.0
3	31.734818296692062	33.0	33.0	33.0	27.0	33.0
4	32.00321670695197	33.0	33.0	33.0	33.0	33.0
5	32.106910283500156	33.0	33.0	33.0	33.0	33.0
6	35.28297321245996	37.0	37.0	37.0	33.0	37.0
7	35.41550704424742	37.0	37.0	37.0	33.0	37.0
8	35.54826633389785	37.0	37.0	37.0	33.0	37.0
9	35.66129150653041	37.0	37.0	37.0	33.0	37.0
10	35.66365488168705	37.0	37.0	37.0	33.0	37.0
11	35.688409368560684	37.0	37.0	37.0	33.0	37.0
12	35.69014486978497	37.0	37.0	37.0	33.0	37.0
13	35.68393560294249	37.0	37.0	37.0	33.0	37.0
14	35.65404277407549	37.0	37.0	37.0	33.0	37.0
15	35.673478028344775	37.0	37.0	37.0	33.0	37.0
16	35.6664717941727	37.0	37.0	37.0	33.0	37.0
17	35.64868946063139	37.0	37.0	37.0	33.0	37.0
18	35.63939718859288	37.0	37.0	37.0	33.0	37.0
19	35.6233569102835	37.0	37.0	37.0	33.0	37.0
20	35.62476471112556	37.0	37.0	37.0	33.0	37.0
21	35.62616464715845	37.0	37.0	37.0	33.0	37.0
22	35.537605585063154	37.0	37.0	37.0	33.0	37.0
23	35.597003245544585	37.0	37.0	37.0	33.0	37.0
24	35.57973474620261	37.0	37.0	37.0	33.0	37.0
25	35.6015228892163	37.0	37.0	37.0	33.0	37.0
26	35.487228860703745	37.0	37.0	37.0	33.0	37.0
27	35.51102515165974	37.0	37.0	37.0	33.0	37.0
28	35.519878305186054	37.0	37.0	37.0	33.0	37.0
29	35.530434189898436	37.0	37.0	37.0	33.0	37.0
30	35.532998117689004	37.0	37.0	37.0	33.0	37.0
31	35.525003539164125	37.0	37.0	37.0	33.0	37.0
32	35.508411413411075	37.0	37.0	37.0	33.0	37.0
33	35.50908516539694	37.0	37.0	37.0	33.0	37.0
34	35.48681071501602	37.0	37.0	37.0	33.0	37.0
35	35.428092049726565	37.0	37.0	37.0	33.0	37.0
36	35.44930344006753	37.0	37.0	37.0	33.0	37.0
37	35.451527870262105	37.0	37.0	37.0	33.0	37.0
38	35.4272007046869	37.0	37.0	37.0	33.0	37.0
39	35.32623621692192	37.0	37.0	37.0	33.0	37.0
40	35.121255695432644	37.0	37.0	37.0	33.0	37.0
41	35.27693566061775	37.0	37.0	37.0	33.0	37.0
42	35.31496987778087	37.0	37.0	37.0	33.0	37.0
43	34.9815701305034	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	26.0
16	40.0
17	40.0
18	29.0
19	31.0
20	52.0
21	115.0
22	347.0
23	773.0
24	1595.0
25	2846.0
26	4724.0
27	7178.0
28	10156.0
29	13539.0
30	18334.0
31	23232.0
32	29940.0
33	39447.0
34	57292.0
35	117323.0
36	435829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.93318582446794	21.08135882929694	14.225342512439507	23.76011283379561
2	15.45015546106133	22.384295549042328	39.781384521006906	22.384164468889438
3	17.46656145299728	27.89503625677029	31.181084609617088	23.45731768061534
4	12.237380913681099	18.2517315688197	40.224697598087275	29.28618991941192
5	13.168967560283761	37.7835919107816	35.65602994919333	13.3914105797413
6	28.05442972268683	40.82661766016684	17.847611457454004	13.27134115969233
7	25.155198901024	33.28806698720133	24.01113132658358	17.54560278519109
8	23.80533548654331	36.34406967172287	21.129596325561153	18.720998516172667
9	25.68830188283532	15.827666301389973	20.581943446778837	37.90208836899588
10	14.49327034495053	28.662117311493635	34.423352191397996	22.421260152157842
11	32.57564635623391	24.550132915275032	24.842048415765273	18.032172312725788
12	22.05292492253163	26.589740094272845	30.94396061303566	20.413374370159865
13	27.395883034557972	22.93234166828332	27.178814301369002	22.492960995789705
14	21.656931780645227	21.817373887784903	28.706291323018203	27.819403008551667
15	23.401870775942076	29.188535205507467	25.593137691835803	21.816456326714658
16	22.05095872023825	29.494607362510028	25.654221043083425	22.800212874168295
17	21.70818412042596	28.228897406185933	26.964105010932084	23.09881346245602
18	22.017140040792142	27.667350031197074	28.771962479617034	21.543547448393742
19	24.09030373893028	26.87641238864741	28.928078941711277	20.105204930711032
20	23.927371108885662	26.233202078406904	28.87079691489752	20.96862989780991
21	22.118071758518898	27.763431783266835	28.242005421475124	21.876491036739147
22	21.73374475023988	27.61072340514778	28.3991705247925	22.256361319819842
23	21.68314781122361	27.51280653093754	28.85808214006701	21.945963517771848
24	22.40867645748022	27.77378711534529	27.941700791199803	21.875835635974685
25	22.5270418355416	27.097675686728923	28.83094854841839	21.544333929311097
26	22.300666411497303	27.370191324591158	28.400612406474313	21.928529857437226
27	22.166964655547574	27.30635529013281	28.177382906099425	22.34929714822019
28	21.479711413935394	27.604955878420533	28.800669033100363	22.11466367454371
29	22.74371732827189	27.543217126408454	27.969227623307102	21.743837922012553
30	22.131704094419653	27.70300383278367	28.450422864573227	21.714869208223444
31	22.449180224723815	27.311074175636918	28.534707402882713	21.70503819675655
32	21.29567487927518	27.28459598475276	28.76947195671209	22.650257179259974
33	21.55783518505896	27.147879385286515	29.114999239735113	22.179286189919413
34	21.833889987049282	27.229935560996836	28.556991028874336	22.379183423079542
35	22.04741955611017	27.774049275651073	28.324585917797013	21.85394525044174
36	22.39897652616622	27.615049050193214	28.13543725717402	21.85053716646655
37	22.68250289687138	26.80825070914363	28.11774143653361	22.39150495745138
38	22.5286147973763	27.437697603330484	28.082349795252803	21.951337804040413
39	21.98148623920555	27.281843301542025	28.49223743334574	22.24443302590668
40	22.12147984249409	27.51241329047886	28.537460086093446	21.828646780933607
41	21.853027689371494	27.143815900546862	28.7014413573612	22.30171505272044
42	21.94124463226774	27.505203882069807	28.30164689104093	22.251904594621518
43	22.03771962479617	27.053501675204355	28.39405839882972	22.51472030116976
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	53.5
2	75.0
3	262.0
4	449.0
5	449.0
6	553.0
7	657.0
8	619.0
9	581.0
10	868.5
11	1156.0
12	1156.0
13	1766.5
14	2377.0
15	4516.5
16	6656.0
17	7610.0
18	8564.0
19	8564.0
20	8186.5
21	7809.0
22	8902.0
23	9995.0
24	12035.5
25	14076.0
26	14076.0
27	17174.0
28	20272.0
29	24663.5
30	29055.0
31	31654.0
32	34253.0
33	34253.0
34	38310.0
35	42367.0
36	44980.0
37	47593.0
38	50959.0
39	54325.0
40	54325.0
41	56697.0
42	59069.0
43	57264.0
44	55459.0
45	55976.5
46	56494.0
47	56494.0
48	55366.0
49	54238.0
50	54852.5
51	55467.0
52	54722.0
53	53977.0
54	53977.0
55	47766.0
56	41555.0
57	36508.5
58	31462.0
59	27821.0
60	24180.0
61	24180.0
62	21148.5
63	18117.0
64	14681.5
65	11246.0
66	9588.0
67	7930.0
68	7930.0
69	6529.5
70	5129.0
71	4314.0
72	3499.0
73	2854.5
74	2210.0
75	2210.0
76	1634.0
77	1058.0
78	824.0
79	590.0
80	479.0
81	368.0
82	368.0
83	288.5
84	209.0
85	196.0
86	183.0
87	142.0
88	101.0
89	101.0
90	75.5
91	50.0
92	29.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	762892.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16113485993222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1547596900114	48.94710212635714
2	7.486466213068191	8.408968772328974
3	1.7689802536062023	2.98043815762005
4	0.7929507368523538	1.7813205307859066
5	0.5166969303210618	1.4509142992737074
6	0.3251340472014005	1.0955938243460053
7	0.24173038657209558	0.9503096988013305
8	0.17662547261110875	0.7935589589609394
9	0.14392285594075177	0.7274583829723642
>10	1.1145510616453338	12.803759184507388
>50	0.16700416675767035	6.598609768434133
>100	0.10597410013795912	10.597480715751587
>500	0.004494437282570114	1.5799706428569025
>1k	7.096479919847547E-4	1.2845149370035642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4586	0.601133581162209	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3289	0.4311226228614273	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1794	0.23515779428805125	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	801	0.10499520246640415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	786	0.1030290001730258	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	765	0.10027631696229611	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.3108015289189034E-4	0.0	0.0
2	0.0	0.0	1.3108015289189034E-4	1.3108015289189034E-4	0.0
3	0.0	0.0	3.93240458675671E-4	1.3108015289189034E-4	0.0
4	0.0	0.0	5.243206115675613E-4	2.6216030578378067E-4	0.0
5	0.0	0.0	9.175610702432324E-4	3.93240458675671E-4	0.0
6	0.0	0.0	0.001966202293378355	3.93240458675671E-4	0.0
7	0.0	0.0	0.003145923669405368	6.554007644594517E-4	0.0
8	0.0	0.0	0.0038013244338648195	6.554007644594517E-4	0.0
9	1.3108015289189034E-4	0.0	0.004194564892540491	7.86480917351342E-4	0.0
10	2.6216030578378067E-4	0.0	0.004849965656999943	7.86480917351342E-4	0.0
11	2.6216030578378067E-4	0.0	0.007602648867729639	7.86480917351342E-4	0.0
12	2.6216030578378067E-4	0.0	0.008782370243756653	9.175610702432324E-4	0.0
13	3.93240458675671E-4	0.0	0.009306690855324214	0.001179721376027013	0.0
14	3.93240458675671E-4	0.0	0.009699931313999885	0.001179721376027013	0.0
15	3.93240458675671E-4	0.0	0.010486412231351227	0.0013108015289189034	0.0
16	5.243206115675613E-4	0.0	0.011272893148702568	0.0013108015289189034	0.0
17	6.554007644594517E-4	0.0	0.011272893148702568	0.001572961834702684	0.0
18	6.554007644594517E-4	0.0	0.01166613360737824	0.001572961834702684	0.0
19	6.554007644594517E-4	0.0	0.01192829391316202	0.0017040419875945744	0.0
20	6.554007644594517E-4	0.0	0.012714774830513363	0.001966202293378355	0.0
21	6.554007644594517E-4	0.0	0.013239095442080923	0.0022283625991621355	0.0
22	7.86480917351342E-4	0.0	0.013501255747864704	0.002490522904945916	0.0
23	7.86480917351342E-4	0.0	0.013763416053648485	0.0030148435165134775	0.0
24	7.86480917351342E-4	0.0	0.013763416053648485	0.003145923669405368	0.0
25	7.86480917351342E-4	0.0	0.013763416053648485	0.003277003822297258	0.0
26	7.86480917351342E-4	0.0	0.013763416053648485	0.0036702442809729294	0.0
27	7.86480917351342E-4	0.0	0.013763416053648485	0.004718885504108052	0.0
28	7.86480917351342E-4	0.0	0.013763416053648485	0.013763416053648485	0.0
29	7.86480917351342E-4	0.0	0.013763416053648485	0.0325078779171888	0.0
30	7.86480917351342E-4	0.0	0.013894496206540376	0.05505366421459394	0.0
31	7.86480917351342E-4	0.0	0.013894496206540376	0.11338433225148514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCGT	35	8.8666484E-4	26.428572	29
ATCCGTC	35	8.8666484E-4	26.428572	30
GGTATCA	670	0.0	25.402985	1
GTATCAA	2035	0.0	24.454546	1
CCGTCCG	95	7.1358954E-9	21.421053	28
ACGGTAC	90	9.46784E-8	20.555555	3
CGCCGTC	105	2.2564564E-8	19.380953	26
TCGCCAT	145	7.2759576E-12	19.13793	13
CGGCCTT	130	6.9485395E-10	18.5	24
GCCGGCA	130	6.9485395E-10	18.5	15
ACTGTAT	80	1.615509E-5	18.5	8
CGTCCGC	110	3.846617E-8	18.5	29
CGTCCTA	50	0.0070327683	18.499998	33
TGTACCG	50	0.0070327683	18.499998	5
CGGTACT	105	4.7920366E-7	17.61905	4
TCACGGT	95	3.6031706E-6	17.526316	1
TGTCCGG	150	2.5102054E-10	17.266666	14
CGAGTCG	140	1.8662831E-9	17.178572	21
CCATCGG	65	0.0015792756	17.076923	19
TCGCCGT	120	1.0398071E-7	16.958334	25
>>END_MODULE