FastQCFastQC Report
Fri 10 Feb 2017
ERR1633352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences860689
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58620.6810822492212635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.4841470031567732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23160.2690867432951972No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC11190.13001211819832714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11190.13001211819832714No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG10840.12594560869257074No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10120.11758021770930033No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC9610.11165473242948382No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA9160.10642636306493983No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9100.10572924714966729No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8870.10305696947445593No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC8690.10096562172863834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10700.024.7242981
GTATCAA28650.022.9877831
CGCTCTC2350.022.82978829
CCGCTCT2400.022.35416628
CAACGAT855.176844E-821.76470621
GCCGGCA2350.021.2553215
AACGATT806.955943E-720.812522
GCCGCTC2500.020.7227
TGTACTA909.471296E-820.5555555
TTCGCCG2450.020.38775424
AGCTTCG2450.019.63265221
CCGGCAG2600.018.516
ACCGTTT701.21874575E-418.510
GCTACCG701.21874575E-418.57
TACCGTT701.21874575E-418.59
GCTTCGC2700.017.81481622
CGACACG1054.793801E-717.6190521
GCCGTCC1502.5102054E-1017.26666627
TAGGACC2550.016.6862744
GTCAACC2800.016.51785715
Produced by FastQC (version 0.11.5)