##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	860689
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89010432339672	33.0	33.0	33.0	33.0	33.0
2	31.994554362841864	33.0	33.0	33.0	33.0	33.0
3	31.720569218382018	33.0	33.0	33.0	27.0	33.0
4	31.992942863217724	33.0	33.0	33.0	33.0	33.0
5	32.0927233878904	33.0	33.0	33.0	33.0	33.0
6	35.25680704644767	37.0	37.0	37.0	33.0	37.0
7	35.41270656415964	37.0	37.0	37.0	33.0	37.0
8	35.554023578783976	37.0	37.0	37.0	33.0	37.0
9	35.655237838522396	37.0	37.0	37.0	33.0	37.0
10	35.6535206096511	37.0	37.0	37.0	33.0	37.0
11	35.67987507682798	37.0	37.0	37.0	33.0	37.0
12	35.67225792359377	37.0	37.0	37.0	33.0	37.0
13	35.68200128036956	37.0	37.0	37.0	33.0	37.0
14	35.65210778806282	37.0	37.0	37.0	33.0	37.0
15	35.67700528297678	37.0	37.0	37.0	33.0	37.0
16	35.66319309297551	37.0	37.0	37.0	33.0	37.0
17	35.657634755411074	37.0	37.0	37.0	33.0	37.0
18	35.642628173474975	37.0	37.0	37.0	33.0	37.0
19	35.62881946905328	37.0	37.0	37.0	33.0	37.0
20	35.63430344758676	37.0	37.0	37.0	33.0	37.0
21	35.63112924645255	37.0	37.0	37.0	33.0	37.0
22	35.52995448994933	37.0	37.0	37.0	33.0	37.0
23	35.586136223421	37.0	37.0	37.0	33.0	37.0
24	35.57450949181412	37.0	37.0	37.0	33.0	37.0
25	35.596395445974096	37.0	37.0	37.0	33.0	37.0
26	35.49135634358055	37.0	37.0	37.0	33.0	37.0
27	35.51217919596974	37.0	37.0	37.0	33.0	37.0
28	35.53125925857075	37.0	37.0	37.0	33.0	37.0
29	35.53529904529975	37.0	37.0	37.0	33.0	37.0
30	35.53189247219379	37.0	37.0	37.0	33.0	37.0
31	35.5210720713289	37.0	37.0	37.0	33.0	37.0
32	35.50710883954599	37.0	37.0	37.0	33.0	37.0
33	35.498253143702314	37.0	37.0	37.0	33.0	37.0
34	35.48003285739681	37.0	37.0	37.0	33.0	37.0
35	35.4157610937284	37.0	37.0	37.0	33.0	37.0
36	35.44110241910841	37.0	37.0	37.0	33.0	37.0
37	35.43389540240435	37.0	37.0	37.0	33.0	37.0
38	35.41193160363383	37.0	37.0	37.0	33.0	37.0
39	35.31297483760104	37.0	37.0	37.0	33.0	37.0
40	35.09740684498117	37.0	37.0	37.0	33.0	37.0
41	35.268295516731364	37.0	37.0	37.0	33.0	37.0
42	35.29271316352364	37.0	37.0	37.0	33.0	37.0
43	34.958764431751774	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	26.0
16	46.0
17	37.0
18	31.0
19	38.0
20	42.0
21	114.0
22	347.0
23	846.0
24	1695.0
25	3185.0
26	5163.0
27	8098.0
28	11339.0
29	15688.0
30	20588.0
31	26688.0
32	34164.0
33	45245.0
34	65507.0
35	133451.0
36	488346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.934543139275625	20.173605100100037	13.746893477202567	24.14495828342177
2	16.15205957087868	21.660437161390465	39.023619449069294	23.16388381866156
3	17.813983912888396	27.760666163968633	30.000731971711037	24.424617951431934
4	12.163162303689253	18.038222865634392	40.139469657448856	29.659145173227497
5	13.607702666119817	37.187416128241445	35.248504395896774	13.956376809741963
6	28.61916441362676	40.47431766875143	17.39931612928712	13.507201788334694
7	26.21899431734343	32.4871120695164	23.51371982214249	17.78017379099768
8	24.641421001081692	35.26767508356677	20.48695870401504	19.603945211336498
9	26.438469644668398	15.09453472741025	20.096341419490663	38.370654208430686
10	15.349795338385874	27.75555398058997	33.53778193981798	23.356868741206174
11	33.696375810542484	24.03585964268162	23.852982900908458	18.41478164586744
12	23.174572929362405	26.13417854765194	29.715727748350453	20.975520774635207
13	28.274905337468002	22.27540958464672	26.041229758948937	23.40845531893634
14	22.25844643070842	21.090661086641052	28.162088745179737	28.488803737470793
15	24.305875873863847	28.118751372446958	25.215147399350986	22.360225354338212
16	22.513009925768774	28.329861308788658	25.433460866817164	23.723667898625404
17	22.31549374977489	27.253165777650228	26.222596082905675	24.208744389669206
18	23.37406426711623	26.4029167329895	28.084360320626843	22.138658679267422
19	25.160307614016215	25.697319240747817	27.984672744742873	21.15770040049309
20	24.453548261915746	25.704522771872302	27.883010007098964	21.95891895911299
21	23.11345910079018	26.751939434569284	27.968174334748092	22.166427129892448
22	22.903162466349634	26.436843040866098	27.930413889337498	22.729580603446774
23	22.73643557661362	26.443349456075303	28.495077780708243	22.325137186602824
24	22.942200957604896	26.886018062273365	27.10770092333003	23.06408005679171
25	22.999132090685485	26.61286480947241	28.470097793744316	21.91790530609779
26	23.02306640377651	26.86707974657513	28.044392341484553	22.065461508163807
27	22.902116792476725	26.75252036449868	27.407344580911342	22.93801826211326
28	22.382997807570444	26.479367111697723	28.513667538448846	22.623967542282987
29	23.865879545341002	26.26581726965257	27.434648287592843	22.433654897413582
30	23.467942543706265	26.1507931436326	28.141291453707435	22.2399728589537
31	23.169693117955497	26.26860573331366	27.983743256855842	22.577957891875
32	21.92615451109518	26.20203116340513	28.45662021938238	23.41519410611731
33	22.089860565198347	26.43091755558628	28.67760596452377	22.801615914691602
34	22.860289837560373	26.198197025871135	28.315338060553813	22.626175076014682
35	22.83647171045523	26.92377850768396	27.798078051421594	22.441671730439218
36	23.49443294848662	26.480877529514146	27.52364675277597	22.50104276922326
37	23.124612955434543	25.971053423478168	27.771471460655363	23.132862160431934
38	23.119849330013512	26.677348031635116	27.247821222299805	22.95498141605156
39	22.35650740279009	26.545593123648615	27.942264859897133	23.15563461366417
40	22.714824983240174	27.00801334744606	28.2229702017802	22.054191467533567
41	22.73550608872659	25.952579851723446	28.009885103678567	23.3020289558714
42	22.011783582687823	27.253630521593745	27.916123013074408	22.818462882644024
43	23.118455098182967	26.115821161883098	27.655634032734238	23.110089707199695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	59.5
2	92.0
3	328.0
4	564.0
5	564.0
6	681.0
7	798.0
8	774.5
9	751.0
10	1015.0
11	1279.0
12	1279.0
13	2100.0
14	2921.0
15	5171.0
16	7421.0
17	8300.0
18	9179.0
19	9179.0
20	8049.0
21	6919.0
22	6884.5
23	6850.0
24	8724.5
25	10599.0
26	10599.0
27	13101.0
28	15603.0
29	18961.5
30	22320.0
31	26501.0
32	30682.0
33	30682.0
34	37310.5
35	43939.0
36	47602.5
37	51266.0
38	55104.0
39	58942.0
40	58942.0
41	62529.5
42	66117.0
43	68080.5
44	70044.0
45	69524.5
46	69005.0
47	69005.0
48	67229.5
49	65454.0
50	65680.5
51	65907.0
52	62395.0
53	58883.0
54	58883.0
55	56360.0
56	53837.0
57	46233.0
58	38629.0
59	35655.5
60	32682.0
61	32682.0
62	28704.5
63	24727.0
64	19787.0
65	14847.0
66	12592.0
67	10337.0
68	10337.0
69	8592.0
70	6847.0
71	5813.5
72	4780.0
73	4351.5
74	3923.0
75	3923.0
76	3121.5
77	2320.0
78	1729.5
79	1139.0
80	785.0
81	431.0
82	431.0
83	344.0
84	257.0
85	223.0
86	189.0
87	153.0
88	117.0
89	117.0
90	82.0
91	47.0
92	31.5
93	16.0
94	9.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	860689.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.94722147648187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.29577470877004	43.45582725095809
2	8.331685245565566	8.489524269563303
3	2.1620281393479694	3.304479793612411
4	0.9550264054967594	1.9462376718692715
5	0.5793515390415682	1.4758175586145708
6	0.38122494013474345	1.1653410874441992
7	0.26626613773001134	0.9495863923432821
8	0.20019009082975492	0.8159303115920438
9	0.17235026320131236	0.7902690327762484
>10	1.3313467806543136	13.78706606564761
>50	0.18101174403655615	6.4530672585717
>100	0.13402218841564548	12.642874861494835
>500	0.008101513979789785	2.757049580049874
>1k	0.001388830967963963	1.275632204431305
>5k	2.314718279939939E-4	0.6912966610313683
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5862	0.6810822492212635	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4167	0.4841470031567732	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2316	0.2690867432951972	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1119	0.13001211819832714	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1119	0.13001211819832714	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1084	0.12594560869257074	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1012	0.11758021770930033	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	961	0.11165473242948382	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	916	0.10642636306493983	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	910	0.10572924714966729	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	887	0.10305696947445593	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	869	0.10096562172863834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.4855795763626585E-4	0.0	0.0
2	2.3237197175751055E-4	0.0	3.4855795763626585E-4	0.0	0.0
3	2.3237197175751055E-4	0.0	6.971159152725317E-4	1.1618598587875527E-4	0.0
4	2.3237197175751055E-4	0.0	8.133019011512869E-4	4.647439435150211E-4	0.0
5	2.3237197175751055E-4	0.0	0.0011618598587875527	4.647439435150211E-4	0.0
6	2.3237197175751055E-4	0.0	0.0017427897881813292	4.647439435150211E-4	0.0
7	3.4855795763626585E-4	0.0	0.005344555350422743	6.971159152725317E-4	0.0
8	3.4855795763626585E-4	0.0	0.0066226011950890505	6.971159152725317E-4	0.0
9	4.647439435150211E-4	0.0	0.008249204997391625	0.0017427897881813292	0.0
10	9.294878870300422E-4	0.0	0.009875808799694198	0.0018589757740600844	0.0
11	9.294878870300422E-4	0.0	0.015685108093631963	0.0019751617599388397	0.0
12	9.294878870300422E-4	0.0	0.016846967952419516	0.0019751617599388397	0.0
13	9.294878870300422E-4	0.0	0.017892641825328313	0.0019751617599388397	0.0
14	9.294878870300422E-4	0.0	0.019286873655873376	0.0019751617599388397	0.0
15	0.0010456738729087975	0.0	0.021262035415812217	0.002091347745817595	0.0
16	0.0011618598587875527	0.0	0.022772453232236034	0.0024399057034538607	0.0
17	0.0011618598587875527	0.0	0.0231210111898723	0.0026722776752113714	0.0
18	0.0011618598587875527	0.0	0.023934313091023587	0.0029046496469688817	0.0
19	0.0011618598587875527	0.0	0.024515243020417365	0.0029046496469688817	0.0
20	0.001278045844666308	0.0	0.024979986963932384	0.0031370216187263924	1.1618598587875527E-4
21	0.001278045844666308	0.0	0.025909474850962427	0.0031370216187263924	1.1618598587875527E-4
22	0.001278045844666308	0.0	0.026258032808598692	0.004066509505756434	1.1618598587875527E-4
23	0.001278045844666308	0.0	0.026722776752113715	0.004415067463392701	1.1618598587875527E-4
24	0.001278045844666308	0.0	0.026955148723871225	0.0047636254210289665	1.1618598587875527E-4
25	0.001278045844666308	0.0	0.02707133470974998	0.005344555350422743	1.1618598587875527E-4
26	0.001278045844666308	0.0	0.02730370668150749	0.00639022922333154	1.1618598587875527E-4
27	0.001278045844666308	0.0	0.02730370668150749	0.008481576969149135	1.1618598587875527E-4
28	0.001278045844666308	0.0	0.02730370668150749	0.02021636154290342	1.1618598587875527E-4
29	0.001278045844666308	0.0	0.02730370668150749	0.0418269549163519	1.1618598587875527E-4
30	0.001278045844666308	0.0	0.027419892667386245	0.07064107941428321	1.1618598587875527E-4
31	0.001278045844666308	0.0	0.027419892667386245	0.13675090537929496	1.1618598587875527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1070	0.0	24.724298	1
GTATCAA	2865	0.0	22.987783	1
CGCTCTC	235	0.0	22.829788	29
CCGCTCT	240	0.0	22.354166	28
CAACGAT	85	5.176844E-8	21.764706	21
GCCGGCA	235	0.0	21.25532	15
AACGATT	80	6.955943E-7	20.8125	22
GCCGCTC	250	0.0	20.72	27
TGTACTA	90	9.471296E-8	20.555555	5
TTCGCCG	245	0.0	20.387754	24
AGCTTCG	245	0.0	19.632652	21
CCGGCAG	260	0.0	18.5	16
ACCGTTT	70	1.21874575E-4	18.5	10
GCTACCG	70	1.21874575E-4	18.5	7
TACCGTT	70	1.21874575E-4	18.5	9
GCTTCGC	270	0.0	17.814816	22
CGACACG	105	4.793801E-7	17.61905	21
GCCGTCC	150	2.5102054E-10	17.266666	27
TAGGACC	255	0.0	16.686274	4
GTCAACC	280	0.0	16.517857	15
>>END_MODULE