FastQCFastQC Report
Fri 10 Feb 2017
ERR1633351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508536
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61901.217219626535781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.8380920918086429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24660.48492142149228373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.2210266333160288No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA7150.14059968222505387No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6770.13312725156134472No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6010.1181823902339264No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5740.11287303160444884No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5270.10363081473091383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG358.8625675E-426.428575
GGTATCA10250.026.3512211
GTATCAA24750.021.7515161
TTCGCCG1251.8189894E-1120.7224
CGAACCT450.00382291820.55555727
ATACCGC450.00382291820.5555576
TCCGTAG555.137676E-420.18181815
TGAGAGC555.137676E-420.1818186
CCGCTCT1303.274181E-1119.92307728
GCCGCTC1303.274181E-1119.92307727
TCCGCCG951.671524E-719.47368431
CCGTCCG1052.2519089E-819.3809528
GCTTTAT2400.019.2708341
GAGTCGC1254.110916E-1019.2422
CGGCAGC1306.9303496E-1018.517
TAGTTCG1103.838977E-818.57
ATGAGAG701.21750156E-418.55
TCGCTAT609.227162E-418.524
CTCGCTA500.007029620518.523
CGTCCGC1103.838977E-818.529
Produced by FastQC (version 0.11.5)