FastQCFastQC Report
Fri 10 Feb 2017
ERR1633350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420174
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124832.970912050721844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90662.1576775335932257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49121.1690394931623567No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA23950.5700019515724438No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.5373964119626631No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19790.47099534954566435No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG18380.43743782337793385No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG15120.359850918905025No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14480.34461913397782823No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.31558354396035926No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12370.2944018430459762No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA10900.25941633704132094No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10550.2510864546592602No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT10550.2510864546592602No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC10460.24894448490387316No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10290.2448985420325865No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA10040.23894862604540024No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9460.22514482095512808No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9440.22466882767615323No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC9260.22038488816537913No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC9070.21586295201511757No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8830.21015103266741872No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA8780.2089610494699815No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8720.20753306963305676No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG8710.2072950729935693No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8440.20086916372740815No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8070.19206328806637252No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.1915872947873976No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT7830.1863513687186737No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7720.18373340568431173No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7640.18182943256841214No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7600.18087744601046235No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT7550.17968746281302508No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7450.17730749641815058No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7430.17683150313917567No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7080.16850162075711492No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.16635965100172784No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT6870.16350369132787845No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6790.16159971821197885No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6780.1613617215724914No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6710.15969574509607923No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6670.15874375853812944No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6640.15802976861966708No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6630.15779177198017963No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6540.15564980222479258No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6520.15517380894581767No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6390.15207985263248083No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.15017587951658123No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6300.14993788287709378No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT6300.14993788287709378No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6210.14779591312170673No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6010.14303598033195772No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5940.14137000385554557No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5880.13994202401862085No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5820.13851404418169616No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5760.13708606434477147No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5700.13565808450784675No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5560.13232613155502246No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5470.1301841617996354No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5430.1292321752416856No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5400.12851818532322323No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT5320.12661421220732363No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5280.12566222564937385No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC5190.12352025589398678No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC5020.11947431302270012No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5000.11899831974372523No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA4960.11804633318577543No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4900.11661835334885072No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4890.11638035670936327No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4880.1161423600698758No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4880.1161423600698758No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA4770.11352439703551387No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4720.11233441383807662No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4610.10971645080371464No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4570.10876446424576486No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4510.10733648440884014No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4480.1066224944903778No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4480.1066224944903778No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4430.10543251129294054No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4390.10448052473499075No Hit
GTATCAACGCAGAGTACGGGCTCCGTCATCTTTAGTGACCATG4340.10329054153755349No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4330.10305254489806603No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC4290.10210055834011623No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10091057514267898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGT251.2322929E-437.04
CTAGTGG251.2322929E-437.09
GTACTAA251.2322929E-437.01
TAGTGCA251.2322929E-437.010
TCCCATG251.2322929E-437.02
GGTTCAG200.001840607537.020
ACTAGTG601.2660166E-930.8333328
TACTAAT303.5956272E-430.8333322
GCGGTGT250.00549242429.618
CTCCCAT250.00549242429.61
GTAGGCT250.00549242429.611
GCCATAC250.00549242429.61
TTGGTAG250.00549242429.64
ACTAATC250.00549242429.63
CGTGTAG250.00549242429.68
GGGTTCA250.00549242429.619
TGTAGGC250.00549242429.610
AACAGCA250.00549242429.630
AGGCTGG250.00549242429.613
AACAACG358.8599924E-426.4285722
Produced by FastQC (version 0.11.5)