FastQCFastQC Report
Fri 10 Feb 2017
ERR1633349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620762
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51550.8304309864328036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36030.5804156826609876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20530.3307225635589807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.15303771815929454No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8950.1441776397395459No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8210.13225680695661138No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7680.1237189132066718No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7380.11888614315953619No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7260.11695303514068195No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG6740.10857623372564687No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6580.10599875636717454No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6240.10052161698042084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCC200.001841284337.04
CTCGTAC250.005494421329.5999983
GGTATCA8250.027.1333351
CGCGTGC400.001930192523.12534
GTATCAA21850.022.437071
GGGCGCG450.003823855420.55555523
GGCGCGT450.003823855420.55555532
ATCAAGC2100.019.38095330
TCGCCTA701.21805104E-418.535
GTCCGGC1205.160473E-918.515
CCGGGCG500.00703132818.49999821
GCGCGGT500.00703132818.49999825
ATAGTGC500.00703132818.4999988
TATCGCC500.00703132818.49999833
TCTATCG500.00703132818.49999831
TAACGGC2200.017.6590936
AACGATT852.7198488E-517.41176422
CGCCGGG1603.45608E-1117.3437520
GCACCGC2250.017.26666610
ACCCGCA1401.8626451E-917.17857226
Produced by FastQC (version 0.11.5)