##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620762
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.883638173728418	33.0	33.0	33.0	33.0	33.0
2	31.985475915085008	33.0	33.0	33.0	33.0	33.0
3	31.702013009816966	33.0	33.0	33.0	27.0	33.0
4	31.986795261307876	33.0	33.0	33.0	33.0	33.0
5	32.081578769319	33.0	33.0	33.0	33.0	33.0
6	35.231781262383976	37.0	37.0	37.0	33.0	37.0
7	35.381477925517345	37.0	37.0	37.0	33.0	37.0
8	35.52107087740551	37.0	37.0	37.0	33.0	37.0
9	35.63195395336699	37.0	37.0	37.0	33.0	37.0
10	35.618940914553406	37.0	37.0	37.0	33.0	37.0
11	35.64106694675254	37.0	37.0	37.0	33.0	37.0
12	35.642903399370454	37.0	37.0	37.0	33.0	37.0
13	35.65454070964395	37.0	37.0	37.0	33.0	37.0
14	35.614604308897775	37.0	37.0	37.0	33.0	37.0
15	35.63681088726436	37.0	37.0	37.0	33.0	37.0
16	35.63774200096011	37.0	37.0	37.0	33.0	37.0
17	35.627032582535655	37.0	37.0	37.0	33.0	37.0
18	35.61018715707469	37.0	37.0	37.0	33.0	37.0
19	35.59459341905593	37.0	37.0	37.0	33.0	37.0
20	35.60498387465728	37.0	37.0	37.0	33.0	37.0
21	35.59669406310309	37.0	37.0	37.0	33.0	37.0
22	35.50253076058135	37.0	37.0	37.0	33.0	37.0
23	35.5500143372178	37.0	37.0	37.0	33.0	37.0
24	35.5459644759183	37.0	37.0	37.0	33.0	37.0
25	35.56870910268348	37.0	37.0	37.0	33.0	37.0
26	35.468914012133475	37.0	37.0	37.0	33.0	37.0
27	35.47324095224901	37.0	37.0	37.0	33.0	37.0
28	35.496058070564885	37.0	37.0	37.0	33.0	37.0
29	35.493245398397455	37.0	37.0	37.0	33.0	37.0
30	35.49184872785383	37.0	37.0	37.0	33.0	37.0
31	35.495025468698145	37.0	37.0	37.0	33.0	37.0
32	35.46448880569365	37.0	37.0	37.0	33.0	37.0
33	35.45434159951801	37.0	37.0	37.0	33.0	37.0
34	35.43716593477049	37.0	37.0	37.0	33.0	37.0
35	35.36336792522738	37.0	37.0	37.0	33.0	37.0
36	35.387974779384045	37.0	37.0	37.0	33.0	37.0
37	35.37411439488886	37.0	37.0	37.0	33.0	37.0
38	35.35238464983359	37.0	37.0	37.0	33.0	37.0
39	35.245847845067836	37.0	37.0	37.0	33.0	37.0
40	35.0294428460505	37.0	37.0	37.0	27.0	37.0
41	35.19293062397505	37.0	37.0	37.0	33.0	37.0
42	35.211805812855815	37.0	37.0	37.0	33.0	37.0
43	34.87169800986529	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	16.0
16	28.0
17	24.0
18	25.0
19	21.0
20	42.0
21	109.0
22	273.0
23	635.0
24	1351.0
25	2441.0
26	4051.0
27	6104.0
28	8521.0
29	11770.0
30	15530.0
31	19641.0
32	24852.0
33	33082.0
34	47236.0
35	95960.0
36	349045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69069627328992	20.391067752214216	13.914350427377965	24.003885547117896
2	15.962961650358753	21.65242073451661	39.348413723778194	23.03620389134644
3	17.496399586314883	28.16957223541389	30.13425435190943	24.199773826361795
4	12.079025455810761	17.986442469094435	40.267445494408484	29.66708658068632
5	13.497765649314875	37.36649472744788	35.128277826284474	14.007461796952777
6	28.38849671854914	40.773114333673774	17.306955000467166	13.531433947309921
7	25.89092115818945	32.954336766747964	23.584078922356717	17.57066315270587
8	24.514387156430324	35.42935940022102	20.552482271788545	19.503771171560114
9	26.370009762195494	15.128825540223145	20.371575579690766	38.129589117890596
10	15.445533070645432	27.821129515015418	33.43922469481057	23.294112719528577
11	33.425692938678594	24.288213518224374	23.95169163060883	18.3344019124882
12	23.35645545313663	26.347134650639052	29.601199815710366	20.69521008051395
13	27.793421633411842	22.534240175783957	26.428647372100738	23.243690818703463
14	22.172104606918595	21.191374472019874	28.50915487739262	28.12736604366891
15	24.10456825643322	28.25060167987087	25.703570772695493	21.941259291000414
16	22.06401165019766	28.596144738241065	25.65492088755433	23.684922724006945
17	21.9763774200096	27.432574803225712	26.57298610417519	24.018061672589493
18	22.88928768191352	26.614870111250365	28.59356726088259	21.902274945953522
19	24.870884493574028	25.757536704888505	28.375448239421875	20.996130562115596
20	23.94766432223622	25.918145762788313	28.64656663906618	21.487623275909286
21	22.981915774483618	26.770485306768133	28.519948063831222	21.727650854917023
22	22.55292688663288	26.688811492971542	28.337913725389118	22.420347895006458
23	22.2864801647008	26.639033961486046	28.9858270963751	22.088658777438052
24	22.701615111749753	27.134392891317443	27.561287578814426	22.60270441811838
25	22.445478299251565	26.62179708165126	29.130649105454264	21.80207551364291
26	22.7425325648155	26.946237044148962	28.7327510382401	21.578479352795433
27	22.770079354084174	26.70008795641486	27.976744710533186	22.553087978967785
28	21.964456587226667	26.425264433067746	29.086187621020613	22.524091358684974
29	23.571513720234165	26.37838656361053	27.97658361819828	22.07351609795703
30	23.227098308208298	26.06119575618353	28.744510778688127	21.967195156920045
31	22.527152113048157	26.339724403233443	28.75804253482011	22.37508094889829
32	21.472963873432974	26.392240504412317	29.031899504157792	23.102896117996913
33	21.67030198369101	26.516764879293515	29.255817849675076	22.557115287340398
34	22.407460508214097	26.500011276463443	28.985343819370385	22.107184395952075
35	22.49912204677477	26.98634903554019	28.410727460765962	22.103801456919076
36	23.357099822476247	26.608587510189093	27.885405356642316	22.14890731069234
37	22.70225948108937	26.335052725521212	28.186325838243963	22.77636195514545
38	22.912968255144484	26.710397865848744	27.86542990711416	22.51120397189261
39	22.114755735692583	26.863113399338236	28.270577129399026	22.751553735570155
40	22.3112883842761	27.184331515137846	28.766419336235145	21.737960764350913
41	22.154706634748905	26.288658133068708	28.582612982109083	22.974022250073297
42	21.675779123077767	27.351867543438544	28.281853592842342	22.69049974064134
43	22.530051775076437	26.129015629178333	28.378186809115245	22.96274578662998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	66.0
2	83.0
3	293.5
4	504.0
5	504.0
6	616.5
7	729.0
8	690.5
9	652.0
10	904.0
11	1156.0
12	1156.0
13	1870.5
14	2585.0
15	4538.0
16	6491.0
17	7281.0
18	8071.0
19	8071.0
20	7043.5
21	6016.0
22	5906.0
23	5796.0
24	7180.5
25	8565.0
26	8565.0
27	10657.5
28	12750.0
29	15095.0
30	17440.0
31	20439.5
32	23439.0
33	23439.0
34	28516.5
35	33594.0
36	36132.0
37	38670.0
38	41225.0
39	43780.0
40	43780.0
41	45376.0
42	46972.0
43	47766.5
44	48561.0
45	48136.0
46	47711.0
47	47711.0
48	45857.5
49	44004.0
50	44561.0
51	45118.0
52	42995.0
53	40872.0
54	40872.0
55	39136.0
56	37400.0
57	32228.5
58	27057.0
59	24663.0
60	22269.0
61	22269.0
62	19696.5
63	17124.0
64	13655.0
65	10186.0
66	8633.5
67	7081.0
68	7081.0
69	5851.5
70	4622.0
71	4035.0
72	3448.0
73	3616.0
74	3784.0
75	3784.0
76	3101.5
77	2419.0
78	1736.0
79	1053.0
80	680.5
81	308.0
82	308.0
83	241.5
84	175.0
85	143.0
86	111.0
87	92.0
88	73.0
89	73.0
90	53.0
91	33.0
92	21.5
93	10.0
94	5.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.05718726838489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1837669998134	47.12909219472149
2	7.138823695512688	7.718094587686257
3	1.8091381840402807	2.9339076482715365
4	0.8575410298635283	1.8542502416662558
5	0.5026485926231432	1.358588455080968
6	0.35291572650668696	1.1446578910638052
7	0.26119976133808204	0.9883807089177098
8	0.21081348123524413	0.9116787067066988
9	0.16947974566161525	0.8245438514485359
>10	1.224937718968178	13.292524614562884
>50	0.1672421379614949	6.371685497561433
>100	0.11482747913783746	11.679949142379883
>500	0.005756522700841437	2.0220241479607624
>1k	6.059497579833091E-4	0.9263379702629251
>5k	3.0297487899165454E-4	0.8442843417088717
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5155	0.8304309864328036	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3603	0.5804156826609876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2053	0.3307225635589807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	950	0.15303771815929454	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	895	0.1441776397395459	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	821	0.13225680695661138	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	768	0.1237189132066718	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	738	0.11888614315953619	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	726	0.11695303514068195	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	674	0.10857623372564687	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	658	0.10599875636717454	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	624	0.10052161698042084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.221846698090411E-4	0.0	0.0
2	0.0	0.0	3.221846698090411E-4	0.0	0.0
3	0.0	0.0	3.221846698090411E-4	0.0	0.0
4	0.0	0.0	4.8327700471356174E-4	1.6109233490452056E-4	0.0
5	0.0	0.0	8.054616745226029E-4	1.6109233490452056E-4	0.0
6	0.0	0.0	0.002255292688663288	1.6109233490452056E-4	0.0
7	0.0	0.0	0.00579932405656274	3.221846698090411E-4	0.0
8	0.0	0.0	0.0074102474056079465	3.221846698090411E-4	0.0
9	1.6109233490452056E-4	0.0	0.009182263089557673	0.0012887386792361645	0.0
10	4.8327700471356174E-4	0.0	0.010793186438602878	0.0012887386792361645	0.0
11	4.8327700471356174E-4	0.0	0.014820494811215893	0.0012887386792361645	0.0
12	4.8327700471356174E-4	0.0	0.015948141155547536	0.0012887386792361645	0.0
13	6.443693396180822E-4	0.0	0.016592510495165618	0.0016109233490452057	0.0
14	6.443693396180822E-4	0.0	0.017397972169688223	0.0016109233490452057	0.0
15	9.665540094271235E-4	0.0	0.01900889551873343	0.0016109233490452057	0.0
16	9.665540094271235E-4	0.0	0.020780911202683154	0.0017720156839497263	0.0
17	9.665540094271235E-4	0.0	0.021425280542301237	0.001933108018854247	0.0
18	9.665540094271235E-4	0.0	0.021586372877205755	0.0020942003537587676	0.0
19	9.665540094271235E-4	0.0	0.021586372877205755	0.0020942003537587676	0.0
20	9.665540094271235E-4	0.0	0.02206964988191932	0.003060754363185891	0.0
21	9.665540094271235E-4	0.0	0.02255292688663288	0.003382939032994932	0.0
22	9.665540094271235E-4	0.0	0.0227140192215374	0.004510585377326576	0.0
23	9.665540094271235E-4	0.0	0.02319729622625096	0.005154954716944658	0.0
24	9.665540094271235E-4	0.0	0.023358388561155484	0.006121508726371782	0.0
25	0.001127646344331644	0.0	0.023358388561155484	0.007249155070703426	0.0
26	0.001127646344331644	0.0	0.023358388561155484	0.008054616745226029	0.0
27	0.001127646344331644	0.0	0.023358388561155484	0.009665540094271235	0.0
28	0.001127646344331644	0.0	0.023358388561155484	0.020942003537587673	0.0
29	0.0012887386792361645	0.0	0.023519480896060003	0.04687786945721548	0.0
30	0.0012887386792361645	0.0	0.023680573230964525	0.08102944445697385	0.0
31	0.0012887386792361645	0.0	0.023841665565869044	0.15803158054133468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTCC	20	0.0018412843	37.0	4
CTCGTAC	25	0.0054944213	29.599998	3
GGTATCA	825	0.0	27.133335	1
CGCGTGC	40	0.0019301925	23.125	34
GTATCAA	2185	0.0	22.43707	1
GGGCGCG	45	0.0038238554	20.555555	23
GGCGCGT	45	0.0038238554	20.555555	32
ATCAAGC	210	0.0	19.380953	30
TCGCCTA	70	1.21805104E-4	18.5	35
GTCCGGC	120	5.160473E-9	18.5	15
CCGGGCG	50	0.007031328	18.499998	21
GCGCGGT	50	0.007031328	18.499998	25
ATAGTGC	50	0.007031328	18.499998	8
TATCGCC	50	0.007031328	18.499998	33
TCTATCG	50	0.007031328	18.499998	31
TAACGGC	220	0.0	17.65909	36
AACGATT	85	2.7198488E-5	17.411764	22
CGCCGGG	160	3.45608E-11	17.34375	20
GCACCGC	225	0.0	17.266666	10
ACCCGCA	140	1.8626451E-9	17.178572	26
>>END_MODULE