FastQCFastQC Report
Fri 10 Feb 2017
ERR1633348.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633348.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586457
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60531.0321302329071014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42270.7207689566327966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23470.40019984414884635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.18756703390018364No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7900.13470723343740462No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7100.12106599460830036No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6120.10435547704264762No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.10316186864510099No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC303.5973475E-430.8333343
GGTATCA8700.026.5804621
CGTAGGG358.864195E-426.4285729
TGCGTTA358.864195E-426.4285737
GCCGGCA1103.6379788E-1223.54545415
GTATCAA25200.023.418651
ATCTCCG652.6791276E-622.7692310
TGCGGTA502.6999164E-422.19999936
TTCGCCG1107.4578566E-1121.86363624
CCGCTCT1201.0913936E-1121.58333428
CGCTCTC1201.0913936E-1121.58333429
CATCTCG705.0948947E-621.14285736
CCCGTAG450.003823606320.55555727
ACTACGC450.003823606320.55555722
TCGACTA450.003823606320.55555719
CGCCAAA450.003823606320.55555724
GTACTGG2250.020.5555551
TAACTGA3100.020.2903216
CTTCGCC1500.019.73333223
ATCTCGT759.25309E-619.73333237
Produced by FastQC (version 0.11.5)