##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633348.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586457
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.920023121899817	33.0	33.0	33.0	33.0	33.0
2	32.020431165456294	33.0	33.0	33.0	33.0	33.0
3	31.747326743478208	33.0	33.0	33.0	27.0	33.0
4	32.012299281959294	33.0	33.0	33.0	33.0	33.0
5	32.11059463865211	33.0	33.0	33.0	33.0	33.0
6	35.271099159870204	37.0	37.0	37.0	33.0	37.0
7	35.41371660667364	37.0	37.0	37.0	33.0	37.0
8	35.54333736318264	37.0	37.0	37.0	33.0	37.0
9	35.64888985893254	37.0	37.0	37.0	33.0	37.0
10	35.64767067321219	37.0	37.0	37.0	33.0	37.0
11	35.67452004153757	37.0	37.0	37.0	33.0	37.0
12	35.66270843386642	37.0	37.0	37.0	33.0	37.0
13	35.683717646818096	37.0	37.0	37.0	33.0	37.0
14	35.642297730268375	37.0	37.0	37.0	33.0	37.0
15	35.66813764692041	37.0	37.0	37.0	33.0	37.0
16	35.65381264099499	37.0	37.0	37.0	33.0	37.0
17	35.647200050472584	37.0	37.0	37.0	33.0	37.0
18	35.632515256873056	37.0	37.0	37.0	33.0	37.0
19	35.612788320371315	37.0	37.0	37.0	33.0	37.0
20	35.619984073853665	37.0	37.0	37.0	33.0	37.0
21	35.61150945423108	37.0	37.0	37.0	33.0	37.0
22	35.524434016475205	37.0	37.0	37.0	33.0	37.0
23	35.5726370390327	37.0	37.0	37.0	33.0	37.0
24	35.565234961813054	37.0	37.0	37.0	33.0	37.0
25	35.58224899694266	37.0	37.0	37.0	33.0	37.0
26	35.47682268265193	37.0	37.0	37.0	33.0	37.0
27	35.4875668633847	37.0	37.0	37.0	33.0	37.0
28	35.5020981930474	37.0	37.0	37.0	33.0	37.0
29	35.507055078206925	37.0	37.0	37.0	33.0	37.0
30	35.50908591763761	37.0	37.0	37.0	33.0	37.0
31	35.49239756708506	37.0	37.0	37.0	33.0	37.0
32	35.48960963889936	37.0	37.0	37.0	33.0	37.0
33	35.479049614890776	37.0	37.0	37.0	33.0	37.0
34	35.43754444059837	37.0	37.0	37.0	33.0	37.0
35	35.36853682367164	37.0	37.0	37.0	33.0	37.0
36	35.39339286597313	37.0	37.0	37.0	33.0	37.0
37	35.367024351316466	37.0	37.0	37.0	33.0	37.0
38	35.3556066344165	37.0	37.0	37.0	33.0	37.0
39	35.24807104357182	37.0	37.0	37.0	33.0	37.0
40	35.025369293912426	37.0	37.0	37.0	27.0	37.0
41	35.16713075297933	37.0	37.0	37.0	33.0	37.0
42	35.18685768948107	37.0	37.0	37.0	33.0	37.0
43	34.845785795036974	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	29.0
16	43.0
17	28.0
18	13.0
19	27.0
20	41.0
21	103.0
22	247.0
23	595.0
24	1223.0
25	2218.0
26	3710.0
27	5589.0
28	7994.0
29	10893.0
30	14276.0
31	18532.0
32	23756.0
33	31148.0
34	44429.0
35	89493.0
36	332066.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.897359908740114	20.489140721314605	14.47352491316499	23.139974456780294
2	15.474450812250515	22.58835686162839	40.008218846394534	21.92897347972656
3	17.575883653874026	28.483759252596524	31.08019854823116	22.860158545298294
4	11.847756954047782	18.350194472911056	40.86130781966965	28.940740753371518
5	13.270708679408722	37.476404919712785	35.13539782115313	14.117488579725368
6	27.227230640950655	41.411902321909366	17.685354595477587	13.675512441662391
7	25.105165425598123	33.6798776380877	23.490895325659	17.724061610655173
8	24.298286148856608	35.089017609134174	21.128914822399597	19.48378141960962
9	26.467243122684188	15.241356143758194	20.669375589344146	37.622025144213474
10	14.943636106312994	28.21281014635344	33.74927744063077	23.094276306702792
11	32.761310718432895	24.860816735071793	24.37348347790205	18.004389068593266
12	22.92648906910481	26.64798953716982	30.58195229999812	19.843569093727247
13	28.31290273626199	23.355335514794774	26.584898807585212	21.74686294135802
14	22.221578052610848	21.956596988355496	29.351512557612917	26.47031240142074
15	23.607527917647843	28.834509605989865	26.160997310970796	21.396965165391496
16	21.202918543047485	29.421082875641353	26.247107631079515	23.128890950231646
17	21.596809314237873	27.641071723928608	27.243429612060222	23.5186893497733
18	22.839526171569272	26.46945982399392	29.25329563804337	21.437718366393444
19	24.547409272973127	25.971895637702335	29.587847020327153	19.892848068997385
20	24.39923131619198	25.66940116666695	29.210496251217055	20.720871265924014
21	22.43114158412296	27.078200106742695	29.188158722634395	21.30249958649995
22	21.865030172715137	26.95116607014666	29.416649473021895	21.767154284116312
23	21.860426254610314	26.552500865366085	29.97662232695662	21.610450553066975
24	21.90561285823172	27.04443804064066	29.227888830724165	21.82206027040346
25	22.053279268556775	26.919620705354358	29.845836949682585	21.181263076406285
26	22.118757214936473	27.055521547189308	29.278361414391846	21.54735982348237
27	22.16206814821888	26.99754628216561	29.162922430800553	21.67746313881495
28	21.5217825006778	26.99635267376807	29.675491979804146	21.806372845749987
29	22.44546488489352	26.904103796186252	28.9855863260222	21.66484499289803
30	22.27818919375163	26.900011424537517	29.628600221329098	21.193199160381752
31	22.18491722325763	26.804693268219154	29.3290045135449	21.68138499497832
32	21.098392550519478	26.74092047669309	29.791954056307624	22.36873291647981
33	21.284765976022115	26.709375111900783	30.23120194660478	21.77465696547232
34	21.57396023919912	26.650888300421006	29.498326390511153	22.276825069868718
35	21.671665612312584	27.45282262808697	29.292855230647767	21.582656528952676
36	22.215610010623116	27.019542779777545	29.26625481493102	21.49859239466832
37	22.367198277111537	26.737169136015087	28.96682962263218	21.928802964241196
38	22.081584839127167	27.07973474611097	28.871511466313816	21.967168948448055
39	21.811147279340172	26.741090992178457	29.219874602912064	22.22788712556931
40	21.62852519451554	27.48863088001337	29.462688654070117	21.420155271400972
41	21.86929305984923	26.540223750419894	29.42398163889254	22.166501550838337
42	21.418961663003426	27.208644453046006	29.36754101323712	22.004852870713453
43	21.72657159859973	26.795996978465595	29.218169448058422	22.25926197487625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	78.0
2	98.0
3	352.5
4	607.0
5	607.0
6	754.5
7	902.0
8	835.0
9	768.0
10	1077.5
11	1387.0
12	1387.0
13	2179.5
14	2972.0
15	5384.5
16	7797.0
17	8826.0
18	9855.0
19	9855.0
20	8907.0
21	7959.0
22	8081.0
23	8203.0
24	9989.0
25	11775.0
26	11775.0
27	13986.0
28	16197.0
29	18806.0
30	21415.0
31	23626.0
32	25837.0
33	25837.0
34	28523.0
35	31209.0
36	32839.0
37	34469.0
38	35937.5
39	37406.0
40	37406.0
41	38651.5
42	39897.0
43	39943.0
44	39989.0
45	40619.0
46	41249.0
47	41249.0
48	40892.0
49	40535.0
50	41385.5
51	42236.0
52	41333.5
53	40431.0
54	40431.0
55	37211.0
56	33991.0
57	30375.5
58	26760.0
59	23615.0
60	20470.0
61	20470.0
62	17692.0
63	14914.0
64	12020.0
65	9126.0
66	7880.0
67	6634.0
68	6634.0
69	5443.0
70	4252.0
71	3620.5
72	2989.0
73	2467.5
74	1946.0
75	1946.0
76	1395.0
77	844.0
78	642.0
79	440.0
80	344.0
81	248.0
82	248.0
83	215.5
84	183.0
85	186.5
86	190.0
87	155.0
88	120.0
89	120.0
90	97.0
91	74.0
92	47.5
93	21.0
94	12.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	586457.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.12152370106133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.5489134106436	53.236955963212374
2	6.383901860138275	7.6761981397910555
3	1.6198393395027388	2.9216162772547634
4	0.7618960040424533	1.832253946591291
5	0.4504888748458485	1.3542038783054564
6	0.322964757934541	1.1650280009261427
7	0.24286951730931777	1.022117980882423
8	0.19640320196644634	0.9446447809592031
9	0.16025782952013592	0.8671450406197949
>10	1.1177256307584986	13.687095578118042
>50	0.1280045966302321	5.344192773630943
>100	0.0641461198529123	7.009604925297523
>500	0.0014382538083612432	0.5649950481334954
>1k	8.62952285016746E-4	1.3271417200417643
>5k	2.8765076167224863E-4	1.0468059462357049
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6053	1.0321302329071014	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4227	0.7207689566327966	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2347	0.40019984414884635	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.18756703390018364	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	790	0.13470723343740462	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	710	0.12106599460830036	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	612	0.10435547704264762	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.10316186864510099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	1.705154853638033E-4	0.0	0.0
2	0.0	0.0	1.705154853638033E-4	0.0	0.0
3	0.0	0.0	6.820619414552132E-4	1.705154853638033E-4	0.0
4	0.0	0.0	0.00102309291218282	1.705154853638033E-4	0.0
5	0.0	0.0	0.00102309291218282	1.705154853638033E-4	0.0
6	0.0	0.0	0.0018756703390018364	1.705154853638033E-4	0.0
7	0.0	0.0	0.005968041987733116	1.705154853638033E-4	0.0
8	0.0	0.0	0.006309072958460723	1.705154853638033E-4	0.0
9	0.0	0.0	0.007502681356007346	6.820619414552132E-4	0.0
10	1.705154853638033E-4	0.0	0.008696289753553969	6.820619414552132E-4	0.0
11	1.705154853638033E-4	0.0	0.011424537519374821	8.525774268190165E-4	0.0
12	1.705154853638033E-4	0.0	0.012447630431557642	8.525774268190165E-4	0.0
13	1.705154853638033E-4	0.0	0.013811754314468069	0.00102309291218282	0.0
14	1.705154853638033E-4	0.0	0.014152785285195675	0.00102309291218282	0.0
15	1.705154853638033E-4	0.0	0.014664331741287085	0.00102309291218282	0.0
16	3.410309707276066E-4	0.0	0.01585794013883371	0.00102309291218282	0.0
17	6.820619414552132E-4	0.0	0.016540002080288923	0.001193608397546623	0.0
18	6.820619414552132E-4	0.0	0.016710517565652724	0.0013641238829104265	0.0
19	6.820619414552132E-4	0.0	0.017222064021744133	0.0013641238829104265	0.0
20	6.820619414552132E-4	0.0	0.017904125963199347	0.0013641238829104265	0.0
21	6.820619414552132E-4	0.0	0.01892721887538217	0.0015346393682742299	0.0
22	6.820619414552132E-4	0.0	0.01909773436074597	0.002387216795093246	0.0
23	6.820619414552132E-4	0.0	0.019609280816837382	0.003751340678003673	0.0
24	6.820619414552132E-4	0.0	0.019779796302201183	0.005627011017005509	0.0
25	6.820619414552132E-4	0.0	0.019779796302201183	0.0061385574730969195	0.0
26	6.820619414552132E-4	0.0	0.019950311787564987	0.006991134899915936	0.0
27	6.820619414552132E-4	0.0	0.019950311787564987	0.009378351695009182	0.0
28	6.820619414552132E-4	0.0	0.019950311787564987	0.019609280816837382	0.0
29	6.820619414552132E-4	0.0	0.019950311787564987	0.045186603621407875	0.0
30	6.820619414552132E-4	0.0	0.019950311787564987	0.0770729993844391	0.0
31	6.820619414552132E-4	0.0	0.019950311787564987	0.14868950323723648	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	30	3.5973475E-4	30.833334	3
GGTATCA	870	0.0	26.580462	1
CGTAGGG	35	8.864195E-4	26.42857	29
TGCGTTA	35	8.864195E-4	26.42857	37
GCCGGCA	110	3.6379788E-12	23.545454	15
GTATCAA	2520	0.0	23.41865	1
ATCTCCG	65	2.6791276E-6	22.76923	10
TGCGGTA	50	2.6999164E-4	22.199999	36
TTCGCCG	110	7.4578566E-11	21.863636	24
CCGCTCT	120	1.0913936E-11	21.583334	28
CGCTCTC	120	1.0913936E-11	21.583334	29
CATCTCG	70	5.0948947E-6	21.142857	36
CCCGTAG	45	0.0038236063	20.555557	27
ACTACGC	45	0.0038236063	20.555557	22
TCGACTA	45	0.0038236063	20.555557	19
CGCCAAA	45	0.0038236063	20.555557	24
GTACTGG	225	0.0	20.555555	1
TAACTGA	310	0.0	20.290321	6
CTTCGCC	150	0.0	19.733332	23
ATCTCGT	75	9.25309E-6	19.733332	37
>>END_MODULE