FastQCFastQC Report
Fri 10 Feb 2017
ERR1633347.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633347.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518607
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25490.4915089846454059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.3702225384539738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10060.19398118421078003No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7280.14037604583046506No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC6570.12668552487721918No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6540.12610705216088483No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG6270.12090079771387581No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC6070.11704431293831359No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5840.11260935544641704No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5720.1102954645810797No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG5360.1033537919850677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA358.8628044E-426.42857229
CGAGTCG809.749783E-1025.43750221
GGTATCA4600.024.532611
GTCCGGC851.9699655E-923.94117515
CGCCGTC851.9699655E-923.94117526
GTATCAA12600.022.6111131
CCGTCCG903.8162398E-922.6111128
GCCGGCA855.1668394E-821.76470615
CCGCTGA603.721212E-521.58333426
CGACACG909.453106E-820.55555536
TCAGAAC450.003823020320.5555553
TACACGA909.453106E-820.55555532
ACGACAC909.453106E-820.55555535
CCGGCAG1001.2856617E-820.34999816
CGTCCGC1001.2856617E-820.34999829
ATCGTAC951.6716876E-719.47368425
CATCGTA951.6716876E-719.47368424
TTCGCCG951.6716876E-719.47368424
TCGCCGT1052.2522727E-819.3809525
GAGTCGC1052.2522727E-819.3809522
Produced by FastQC (version 0.11.5)