FastQCFastQC Report
Fri 10 Feb 2017
ERR1633346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571227
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124482.179168701759546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90521.5846589884581788No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44480.7786746774924855No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA36460.6382751515597127No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA32510.5691257591115266No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT32260.564749215285692No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG28000.49017290849347106No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA26690.467239818846098No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC26280.46006228697172924No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.404567711260147No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG21290.3727064722080714No Hit
GTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAA20150.35274943236226575No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA19410.33979486263779546No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19260.3371689363422947No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT17720.31020942637515386No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC17000.2976049801567503No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC16650.2914778188005819No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC16290.2851755956913801No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC16050.28097411361857894No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15740.2755471992745441No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC15440.27029534668354266No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT15220.2664439881168082No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC14670.25681559169997215No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA14610.25576522118177186No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13950.24421114548156864No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA13900.24333583671640172No Hit
GTATGAAACTGCTTGTCCGGCGAGTCGCCGTCCGCCGCCTGGG13500.2363333665950664No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT13470.2358081813359663No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA12880.22547953790699668No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12790.22390398212969626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.22372892037666287No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12510.21900225304476154No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA12400.21707657376139433No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT12150.21270002993555975No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC12020.2104242271461258No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG11720.20517237455512433No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC11410.19974546021108947No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC11380.1992202749519893No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11340.1985200279398558No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11330.19834496618682237No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG11250.19694447216255534No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11230.19659434865648856No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA11090.19414348411402124No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC11050.19344323710188768No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA10850.18994200204122003No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT10790.18889163152301974No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG10760.1883664462639196No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG10610.18574051996841887No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT10570.18504027295628533No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG10490.18363977893201827No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA10460.1831145936729181No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG10400.18206422315471782No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC10400.18206422315471782No Hit
GAGTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTG10220.17891311160011694No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG10200.17856298809405016No Hit
GGAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAA10110.17698743231674974No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10090.17663730881068296No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9910.17348619725608208No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG9810.17173557972574827No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9790.1713854562196815No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA9730.1703350857014812No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA9670.1692847151832809No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9560.16735903589991372No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG9380.1642079243453128No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG9230.16158199804981208No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8970.15703039247094414No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG8740.15300397215117634No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8610.15072816936174235No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT8300.1453012550177075No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC8150.14267532872220676No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG8020.14039952593277277No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7980.13969927892063927No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC7890.13812372314333882No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC7850.13742347613120529No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA7840.1372484143781719No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT7820.13689829087210514No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA7740.13549779684783805No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7580.13269680879930396No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7540.13199656178717042No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7520.13164643828110367No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA7340.12849532672650277No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.1272698944552691No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT7240.12674470919616895No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG7110.12446890640673497No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC7100.1242938446537016No Hit
GGTATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAA7040.12324347413550128No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6910.12096767134606731No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC6870.1202674243339338No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG6840.11974223907483365No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC6750.1181666832975332No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA6720.11764149803843306No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6700.11729137453236628No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG6690.1171163127793329No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6650.11641606576719937No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA6560.11484050998989893No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA6550.11466544823686556No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC6510.11396520122473203No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC6400.11203952194136482No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG6380.11168939843529806No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.11133927492923128No Hit
AAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAG6320.11063902791709776No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG6280.10993878090496423No Hit
GTACGGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCG6270.10976371915193085No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA6260.10958865739889745No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA6240.10923853389283071No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA6110.10696273110339673No Hit
GAGCAAAGGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGT6100.10678766935036334No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA6070.1062624840912632No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5870.10276124903059554No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5820.10188594026542862No Hit
GTATCAACGCAGAGTACGGGGGTTAAGCGACTAAGCGTACACG5820.10188594026542862No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA5770.10101063150026171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGT200.001841161337.011
CAAGTAT200.001841161337.014
GCAAAGC200.001841161337.030
TGAAATA200.001841161337.021
TTGAAAT200.001841161337.020
ATCGGTT200.001841161337.012
GTCCAAT200.001841161337.023
AACGCCC251.2328486E-436.99999620
GTATTAT401.5982023E-632.3751
TCCCGTG352.38622E-531.71428722
AGATCTT303.597231E-430.8333348
GGGAACA303.597231E-430.83333417
TAACGCC250.005494058629.59999819
TGTTCTA250.005494058629.59999816
GGAACAG250.005494058629.59999818
CTTGAAA250.005494058629.59999819
TATTACT250.005494058629.5999984
TAGATTT250.005494058629.5999988
GTTCAAA250.005494058629.59999816
AGTTAAG250.005494058629.59999827
Produced by FastQC (version 0.11.5)