FastQCFastQC Report
Fri 10 Feb 2017
ERR1633345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537763
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49110.9132275742287959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.6218352694402552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19480.36224135911172767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.1651285045642783No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7580.14095428655374206No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG7070.13147055487268555No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG6300.11715197958952178No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6270.11659411302004787No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG5780.10748229238530728No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5430.1009738490747783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT200.001841065137.033
ACAACGC250.00549377429.63
AGCCCGA250.00549377429.66
GGTATCA7950.027.2264181
CCGGCGT358.8632305E-426.42857223
ACGCTAT358.8632305E-426.42857210
TCCGGCG856.91216E-1126.11764716
CGAGTCG851.9699655E-923.94117721
GTCCGGC952.8012437E-1023.36842215
GCGTCAA400.00192985823.12500226
GCTTCGC1300.022.7692322
GGCGAGT903.8162398E-922.6111119
GTATCAA21050.022.5866991
TTCGCCG1250.022.224
GCCGCTC1301.8189894E-1221.34615327
GCCGGCA1400.021.14285915
AACTGCT2850.020.7719297
TGCTCGC1450.020.41379410
GAGTCGC1001.2860255E-820.3522
AGCTTCG1500.019.73333421
Produced by FastQC (version 0.11.5)