FastQCFastQC Report
Fri 10 Feb 2017
ERR1633344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810718
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66600.8214940336837223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45930.5665348493557563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24310.29985765703980916No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA13290.1639287643792293No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG12950.15973495099405713No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG12340.15221075639124823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.13740906209064066No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG11010.1358055452080748No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG8930.11014927508702162No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT8440.10410524991427351No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC8290.10225503812669758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCCG303.598551E-430.8333345
GGTATCA10000.024.7899991
GTATCAA28300.023.7950531
CGCTCTC1900.023.36842229
CCGCTCT1850.023.028
GCCGGCA2100.022.9047615
CCGGCAG2100.022.0238116
TTCGCCG2100.021.14285724
CTTCGCC2250.020.55555523
ACGATCA450.003824850520.55555516
CGACAGC555.1412964E-420.18181836
GCCGCTC2200.020.18181827
AGCTTCG2300.020.10869421
GCTTCGC2300.020.10869422
TGCTCGC2700.019.87036910
TCCATCG1800.019.5277772
GCGACAG609.2336145E-418.535
CCATAAG701.218635E-418.51
ACCGTAG609.2336145E-418.58
GGACCGT609.2336145E-418.56
Produced by FastQC (version 0.11.5)