##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810718
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916719500492157	33.0	33.0	33.0	33.0	33.0
2	31.996627680648512	33.0	33.0	33.0	33.0	33.0
3	31.71383761061183	33.0	33.0	33.0	27.0	33.0
4	31.991850433812992	33.0	33.0	33.0	33.0	33.0
5	32.095876741357664	33.0	33.0	33.0	33.0	33.0
6	35.25155109421525	37.0	37.0	37.0	33.0	37.0
7	35.4050372632654	37.0	37.0	37.0	33.0	37.0
8	35.54180615207754	37.0	37.0	37.0	33.0	37.0
9	35.66637597783693	37.0	37.0	37.0	33.0	37.0
10	35.665591488039	37.0	37.0	37.0	33.0	37.0
11	35.686242318537396	37.0	37.0	37.0	33.0	37.0
12	35.672632160627984	37.0	37.0	37.0	33.0	37.0
13	35.67077454799326	37.0	37.0	37.0	33.0	37.0
14	35.65540052151303	37.0	37.0	37.0	33.0	37.0
15	35.67318352374068	37.0	37.0	37.0	33.0	37.0
16	35.65926623067454	37.0	37.0	37.0	33.0	37.0
17	35.65866922900441	37.0	37.0	37.0	33.0	37.0
18	35.64225168307599	37.0	37.0	37.0	33.0	37.0
19	35.62978742300035	37.0	37.0	37.0	33.0	37.0
20	35.630040285277985	37.0	37.0	37.0	33.0	37.0
21	35.61790782984959	37.0	37.0	37.0	33.0	37.0
22	35.530301535182396	37.0	37.0	37.0	33.0	37.0
23	35.581820065670186	37.0	37.0	37.0	33.0	37.0
24	35.574103449041466	37.0	37.0	37.0	33.0	37.0
25	35.5986014865835	37.0	37.0	37.0	33.0	37.0
26	35.48467161207719	37.0	37.0	37.0	33.0	37.0
27	35.49470222691491	37.0	37.0	37.0	33.0	37.0
28	35.512421088467256	37.0	37.0	37.0	33.0	37.0
29	35.52112571819054	37.0	37.0	37.0	33.0	37.0
30	35.52424147484082	37.0	37.0	37.0	33.0	37.0
31	35.5065929213364	37.0	37.0	37.0	33.0	37.0
32	35.503722626116605	37.0	37.0	37.0	33.0	37.0
33	35.48983493643906	37.0	37.0	37.0	33.0	37.0
34	35.46102960585555	37.0	37.0	37.0	33.0	37.0
35	35.39801632626882	37.0	37.0	37.0	33.0	37.0
36	35.427997158074696	37.0	37.0	37.0	33.0	37.0
37	35.40684553691913	37.0	37.0	37.0	33.0	37.0
38	35.40627073779045	37.0	37.0	37.0	33.0	37.0
39	35.302016237458645	37.0	37.0	37.0	33.0	37.0
40	35.07165253516019	37.0	37.0	37.0	33.0	37.0
41	35.23485354956964	37.0	37.0	37.0	33.0	37.0
42	35.26956845660266	37.0	37.0	37.0	33.0	37.0
43	34.93610478612785	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	21.0
16	31.0
17	37.0
18	27.0
19	23.0
20	53.0
21	133.0
22	344.0
23	818.0
24	1662.0
25	3030.0
26	5142.0
27	7616.0
28	10754.0
29	14678.0
30	19418.0
31	25242.0
32	32519.0
33	42309.0
34	61823.0
35	125761.0
36	459274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44632535604242	20.485174869683416	13.822562222622418	23.245937551651746
2	15.992860649449007	22.451703304971645	39.31342340986632	22.242012635713035
3	17.772887736549578	27.87529079161928	30.22752671089084	24.124294760940302
4	11.84776950801635	18.14835738197499	40.48917132714458	29.514701782864076
5	13.848835230005996	36.77123241373696	35.01538142732738	14.364550928929665
6	28.39409017685558	40.85526656617961	17.361400634992684	13.389242621972128
7	26.590133683969025	32.65747152524059	23.187717554069355	17.564677236721028
8	24.530354574586973	34.95728477719749	20.818089643994583	19.69427100422095
9	26.41831068262947	15.060970645773253	20.51107783470948	38.0096408368878
10	15.158908523062273	28.021827565195295	33.789184401974545	23.030079509767884
11	33.45318594135075	24.57031914919861	23.951978369790726	18.024516539659906
12	23.14960319124529	25.98930824281686	30.48507619172141	20.376012374216433
13	28.286531198271163	22.900811379542578	26.42558818232727	22.387069239858988
14	22.450716525351602	21.502174615587663	28.432697929489663	27.614410929571072
15	23.503116990124802	28.933981976470243	25.82661295296268	21.736288080442275
16	22.730961937443105	28.814951684802853	25.358632718158468	23.09545365959557
17	22.422099916370428	27.137673025639003	26.441870046057943	23.998357011932633
18	23.120739887359107	26.179756709484682	28.71602702789379	21.983476375262423
19	25.405258055205387	26.026189131115874	28.270372681993	20.29818013168574
20	24.82367481664401	26.0487617149243	28.193897261439858	20.933666206991827
21	22.683226473323646	27.013215446061395	28.239782513771743	22.06377556684322
22	23.034643365510572	26.060726417817293	28.724661349569146	22.179968867102986
23	22.079440693311362	26.572124955903288	29.112095697887554	22.236338652897803
24	22.289871447284998	27.360438524863145	28.04600366588629	22.303686361965568
25	22.4677384737973	27.076245994291483	29.097540698491954	21.358474833419265
26	23.28281843995076	26.7454281266729	27.888982358847343	22.082771074529
27	22.823225831916893	26.65809813029931	27.86172257184372	22.65695346594007
28	22.409148433857396	26.429411953354926	28.716273722798803	22.445165889988875
29	23.28109157561569	26.961656210914274	28.148012009107976	21.60924020436206
30	23.186360732091803	26.439033054650324	28.274813190283183	22.099793022974694
31	22.648319144264715	26.169272176021746	28.464521572235967	22.71788710747757
32	21.7003939717633	26.53203703383914	28.703198892833264	23.06437010156429
33	22.054894550262855	26.25191496920014	29.0084838377833	22.684706642753707
34	22.741446470906038	26.41855737753448	28.775110457643716	22.064885693915766
35	22.707279226562132	27.01901277632913	27.897986722880212	22.375721274228525
36	22.803490239516083	26.60197503940951	27.792771345893392	22.80176337518101
37	22.90179815916262	26.377112633492782	28.50571962137266	22.215369585971942
38	22.647332364644672	26.38093640452044	27.773899185660117	23.19783204517477
39	22.242259330618044	26.350469583751686	28.529649027158644	22.877622058471626
40	22.70382549789199	26.93883693220084	28.39236331252051	21.964974257386665
41	22.70518231986955	26.3678615745549	28.13444378933242	22.792512316243133
42	21.863952693785016	27.985686761611312	27.822744776852126	22.32761576775155
43	23.121726666979146	26.062329934699857	27.767855160487372	23.048088237833625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	68.0
2	93.0
3	384.5
4	676.0
5	676.0
6	801.5
7	927.0
8	855.0
9	783.0
10	1075.5
11	1368.0
12	1368.0
13	2219.0
14	3070.0
15	5603.5
16	8137.0
17	9307.0
18	10477.0
19	10477.0
20	9247.5
21	8018.0
22	8431.5
23	8845.0
24	10988.5
25	13132.0
26	13132.0
27	15711.5
28	18291.0
29	21475.5
30	24660.0
31	27958.5
32	31257.0
33	31257.0
34	38386.5
35	45516.0
36	48296.0
37	51076.0
38	52574.0
39	54072.0
40	54072.0
41	54386.5
42	54701.0
43	56417.0
44	58133.0
45	58256.5
46	58380.0
47	58380.0
48	56744.0
49	55108.0
50	56533.0
51	57958.0
52	58063.5
53	58169.0
54	58169.0
55	54967.5
56	51766.0
57	44260.0
58	36754.0
59	33862.5
60	30971.0
61	30971.0
62	27707.5
63	24444.0
64	18876.5
65	13309.0
66	11503.5
67	9698.0
68	9698.0
69	8225.0
70	6752.0
71	6564.0
72	6376.0
73	5686.0
74	4996.0
75	4996.0
76	3089.5
77	1183.0
78	902.0
79	621.0
80	503.0
81	385.0
82	385.0
83	313.5
84	242.0
85	209.0
86	176.0
87	133.0
88	90.0
89	90.0
90	70.5
91	51.0
92	32.0
93	13.0
94	6.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	810718.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.7623070092169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.75429899193512	48.93382161766898
2	7.159641750438849	7.984762827279564
3	1.6684111283890888	2.79103360676479
4	0.7334401858071988	1.6359326725551242
5	0.46322514080121885	1.2915251257872644
6	0.30802449389294095	1.0305693836890097
7	0.23500888897002975	0.9173246471649235
8	0.18420497287590415	0.8217355400104509
9	0.14788270813071303	0.7421652874925312
>10	1.0895542828164724	12.053594268155479
>50	0.140171909201751	5.461014797625183
>100	0.1080487388441378	11.619697609531311
>500	0.006289739475115412	2.2420439772868628
>1k	0.001572434868778853	1.6405413693796327
>5k	2.2463355268269327E-4	0.8342372696089451
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6660	0.8214940336837223	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4593	0.5665348493557563	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.29985765703980916	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1329	0.1639287643792293	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1295	0.15973495099405713	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1234	0.15221075639124823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.13740906209064066	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1101	0.1358055452080748	No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG	893	0.11014927508702162	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	844	0.10410524991427351	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	829	0.10225503812669758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	4.933898100202537E-4	0.0	0.0
2	0.0	0.0	6.16737262525317E-4	1.2334745250506342E-4	0.0
3	0.0	0.0	8.634321675354439E-4	1.2334745250506342E-4	0.0
4	0.0	0.0	8.634321675354439E-4	2.4669490501012684E-4	0.0
5	0.0	0.0	0.001233474525050634	3.7004235751519026E-4	0.0
6	0.0	0.0	0.0028369914076164585	3.7004235751519026E-4	0.0
7	0.0	0.0	0.006660762435273425	3.7004235751519026E-4	0.0
8	1.2334745250506342E-4	0.0	0.007647542055313932	3.7004235751519026E-4	0.0
9	1.2334745250506342E-4	0.0	0.009867796200405073	8.634321675354439E-4	0.0
10	4.933898100202537E-4	0.0	0.01085457582044558	8.634321675354439E-4	0.0
11	7.400847150303805E-4	0.0	0.01554177901563799	8.634321675354439E-4	0.0
12	7.400847150303805E-4	0.0	0.016405211183173434	9.867796200405073E-4	0.0
13	7.400847150303805E-4	0.0	0.01726864335070888	9.867796200405073E-4	0.0
14	7.400847150303805E-4	0.0	0.01911885513828483	9.867796200405073E-4	0.0
15	7.400847150303805E-4	0.0	0.020845719473355718	9.867796200405073E-4	0.0
16	7.400847150303805E-4	0.0	0.022202541450911415	9.867796200405073E-4	1.2334745250506342E-4
17	7.400847150303805E-4	0.0	0.022695931260931668	9.867796200405073E-4	1.2334745250506342E-4
18	7.400847150303805E-4	0.0	0.023682710880972176	9.867796200405073E-4	1.2334745250506342E-4
19	7.400847150303805E-4	0.0	0.024422795596002557	0.001233474525050634	1.2334745250506342E-4
20	7.400847150303805E-4	0.0	0.02466949050101268	0.0013568219775556974	1.2334745250506342E-4
21	7.400847150303805E-4	0.0	0.025162880311032938	0.0016035168825658244	1.2334745250506342E-4
22	7.400847150303805E-4	0.0	0.025409575216043062	0.0018502117875759511	1.2334745250506342E-4
23	7.400847150303805E-4	0.0	0.025902965026063318	0.002713643955111395	1.2334745250506342E-4
24	7.400847150303805E-4	0.0	0.026149659931073443	0.003577076122646839	1.2334745250506342E-4
25	7.400847150303805E-4	0.0	0.02639635483608357	0.003823771027656966	1.2334745250506342E-4
26	7.400847150303805E-4	0.0	0.026519702288588635	0.004440508290182283	1.2334745250506342E-4
27	7.400847150303805E-4	0.0	0.026519702288588635	0.006660762435273425	1.2334745250506342E-4
28	7.400847150303805E-4	0.0	0.026519702288588635	0.018378770423254448	1.2334745250506342E-4
29	7.400847150303805E-4	0.0	0.026519702288588635	0.04477512525933802	1.2334745250506342E-4
30	7.400847150303805E-4	0.0	0.026519702288588635	0.07598203074311906	1.2334745250506342E-4
31	7.400847150303805E-4	0.0	0.026519702288588635	0.15011384969866218	1.2334745250506342E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCCG	30	3.598551E-4	30.833334	5
GGTATCA	1000	0.0	24.789999	1
GTATCAA	2830	0.0	23.795053	1
CGCTCTC	190	0.0	23.368422	29
CCGCTCT	185	0.0	23.0	28
GCCGGCA	210	0.0	22.90476	15
CCGGCAG	210	0.0	22.02381	16
TTCGCCG	210	0.0	21.142857	24
CTTCGCC	225	0.0	20.555555	23
ACGATCA	45	0.0038248505	20.555555	16
CGACAGC	55	5.1412964E-4	20.181818	36
GCCGCTC	220	0.0	20.181818	27
AGCTTCG	230	0.0	20.108694	21
GCTTCGC	230	0.0	20.108694	22
TGCTCGC	270	0.0	19.870369	10
TCCATCG	180	0.0	19.527777	2
GCGACAG	60	9.2336145E-4	18.5	35
CCATAAG	70	1.218635E-4	18.5	1
ACCGTAG	60	9.2336145E-4	18.5	8
GGACCGT	60	9.2336145E-4	18.5	6
>>END_MODULE