FastQCFastQC Report
Fri 10 Feb 2017
ERR1633343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489272
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23940.4892983861737438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.3405058944717867No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9950.20336336434539481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATCG358.8620855E-426.4285731
GGTATCA3800.025.8026311
GTAATGG451.3216949E-424.6666681
GTATCAA10300.024.4271831
GTCGCCC753.731693E-722.237
TAACCTG701.21738194E-418.55
GTTGGCG500.00702924918.59
GTTCTAG1258.541974E-917.761
TATCAAC15150.016.2409252
ATCAACG15150.016.2409253
ATTAGCC803.3776014E-416.18753
TCTAGAC1151.2385553E-616.0869563
CAACGCA15700.015.9076435
TCAACGC15700.015.9076434
GGTCGCC1059.321098E-615.85714236
ATAATAT1403.4584446E-815.8571423
CGAGCCC700.002589641115.85714217
GCACCGA957.046434E-515.57894813
CTACACT957.046434E-515.5789484
AACGCAG16400.015.45426756
Produced by FastQC (version 0.11.5)