##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489272
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83358745237823	33.0	33.0	33.0	27.0	33.0
2	31.94969260452264	33.0	33.0	33.0	33.0	33.0
3	31.66129882764597	33.0	33.0	33.0	27.0	33.0
4	31.948411108749326	33.0	33.0	33.0	33.0	33.0
5	32.04246104416357	33.0	33.0	33.0	33.0	33.0
6	35.2090677578116	37.0	37.0	37.0	33.0	37.0
7	35.34113948887327	37.0	37.0	37.0	33.0	37.0
8	35.465274939093185	37.0	37.0	37.0	33.0	37.0
9	35.59709118854134	37.0	37.0	37.0	33.0	37.0
10	35.603668307199264	37.0	37.0	37.0	33.0	37.0
11	35.6156677676221	37.0	37.0	37.0	33.0	37.0
12	35.607077453849804	37.0	37.0	37.0	33.0	37.0
13	35.605058127176704	37.0	37.0	37.0	33.0	37.0
14	35.589506450399774	37.0	37.0	37.0	33.0	37.0
15	35.61332346833663	37.0	37.0	37.0	33.0	37.0
16	35.59233514282444	37.0	37.0	37.0	33.0	37.0
17	35.58340759332232	37.0	37.0	37.0	33.0	37.0
18	35.56718553279158	37.0	37.0	37.0	33.0	37.0
19	35.54112232050884	37.0	37.0	37.0	33.0	37.0
20	35.55047294756291	37.0	37.0	37.0	33.0	37.0
21	35.549254811229744	37.0	37.0	37.0	33.0	37.0
22	35.465612174823	37.0	37.0	37.0	33.0	37.0
23	35.50318227897775	37.0	37.0	37.0	33.0	37.0
24	35.50302081459802	37.0	37.0	37.0	33.0	37.0
25	35.523626939616406	37.0	37.0	37.0	33.0	37.0
26	35.4175959384555	37.0	37.0	37.0	33.0	37.0
27	35.42003221112183	37.0	37.0	37.0	33.0	37.0
28	35.44295606533789	37.0	37.0	37.0	33.0	37.0
29	35.451521852875295	37.0	37.0	37.0	33.0	37.0
30	35.44915098350202	37.0	37.0	37.0	33.0	37.0
31	35.436311499534	37.0	37.0	37.0	33.0	37.0
32	35.43824089667915	37.0	37.0	37.0	33.0	37.0
33	35.422388773524744	37.0	37.0	37.0	33.0	37.0
34	35.41300340097124	37.0	37.0	37.0	33.0	37.0
35	35.35029186219526	37.0	37.0	37.0	33.0	37.0
36	35.376847643028825	37.0	37.0	37.0	33.0	37.0
37	35.38499444072009	37.0	37.0	37.0	33.0	37.0
38	35.36324171422031	37.0	37.0	37.0	33.0	37.0
39	35.27241084713615	37.0	37.0	37.0	33.0	37.0
40	35.06658055233081	37.0	37.0	37.0	33.0	37.0
41	35.23685598194869	37.0	37.0	37.0	33.0	37.0
42	35.2801427426871	37.0	37.0	37.0	33.0	37.0
43	34.93739065386942	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	14.0
16	24.0
17	22.0
18	10.0
19	19.0
20	38.0
21	84.0
22	226.0
23	579.0
24	1131.0
25	2017.0
26	3388.0
27	4995.0
28	7003.0
29	9475.0
30	12149.0
31	15676.0
32	20014.0
33	25873.0
34	37307.0
35	76186.0
36	273041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27254369757517	20.542561192956065	13.775772985169803	24.40912212429896
2	16.157883549436715	21.578590232018183	38.824621069670854	23.438905148874245
3	17.96076619957815	26.53881685442862	30.343449042659298	25.156967903333932
4	12.492642129531221	17.673196095423403	39.333949214342944	30.500212560702433
5	13.857527101489561	37.00027796399549	35.1816576464625	13.960537288052455
6	29.682875782795666	39.77235566310764	17.14342942167138	13.401339132425319
7	26.674733072810213	32.334979316208575	23.170751647345444	17.81953596363577
8	24.635376641213885	35.841617750453736	20.471639497048677	19.051366111283702
9	25.693070521100736	15.52735492732059	20.457945682565118	38.32162886901356
10	14.661374450203567	28.38789058029072	33.671659118036594	23.279075851469123
11	33.764654425350315	23.690503441848296	24.36558805735869	18.1792540754427
12	23.33957389754574	25.700837162151114	30.2749799702415	20.684608970061642
13	28.09541522915679	22.331341258032342	26.328913160777645	23.244330352033224
14	22.20727938651711	21.030020111512616	27.752661096486207	29.01003940548407
15	23.475490115927336	28.857772363838517	24.60758024166517	23.059157278568975
16	23.04403276704982	28.56427508625059	25.00960610866757	23.382086038032014
17	22.421270785984074	27.61286155757943	26.61014732091761	23.355720335518892
18	23.235337399238052	26.931236612763453	27.85219673310551	21.981229254892984
19	24.74145260713877	26.325438610834055	27.430958648767966	21.50215013325921
20	24.10949328798705	26.37428669533511	27.696250756225577	21.81996926045226
21	23.15889730047908	26.68965319903857	27.91719125557972	22.23425824490263
22	22.780784512500205	26.664718193561043	27.721185761703104	22.833311532235648
23	22.647525302899002	26.319511437400873	28.106860805441553	22.926102454258572
24	22.680022564136102	27.009311793848823	27.17731650288592	23.133349139129155
25	23.148064880066713	26.75383018034958	27.84361255089194	22.254492388691773
26	23.401502640657956	26.636717408721527	27.501880344675357	22.45989960594516
27	23.146225412449517	26.880753445936	27.180382282247912	22.79263885936657
28	22.544923886917704	26.561299236416552	27.959294625484393	22.934482251181347
29	23.43052535195147	26.493034549289558	27.258253078042475	22.818187020716493
30	22.888086790169883	26.728077633708857	27.749799702415018	22.63403587370624
31	23.045259078794615	26.687609346130575	27.55052404388561	22.716607531189194
32	22.478907437989506	26.65715593780147	27.88428522376102	22.979651400448013
33	22.59377197141876	26.48894684347357	28.24952173841953	22.66775944668814
34	23.063040599094165	26.26105724423225	27.719755064667506	22.95614709200608
35	22.817778250134893	27.018304746643995	27.749595317124214	22.414321686096894
36	23.6557579424124	26.365702513121537	27.378431629032523	22.600107915433544
37	23.083479128174105	26.20648637158881	27.624920289736586	23.0851142105005
38	23.118633398191598	26.624658676564366	27.519048709102506	22.73765921614153
39	22.801631812161745	26.61934465900358	27.73528834676826	22.843735182066418
40	22.86540002289115	26.95453653591458	27.754500564103402	22.42556287709086
41	23.00070308540035	26.194836410013245	27.77800487254533	23.026455632041074
42	22.578238689318006	26.751990712732386	27.66027894504488	23.009491652904725
43	23.047098546411814	26.290284340816562	27.590992331463887	23.07162478130774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	38.0
2	41.0
3	156.5
4	272.0
5	272.0
6	309.0
7	346.0
8	320.0
9	294.0
10	382.5
11	471.0
12	471.0
13	769.5
14	1068.0
15	2083.0
16	3098.0
17	3632.0
18	4166.0
19	4166.0
20	4139.5
21	4113.0
22	4593.5
23	5074.0
24	6360.5
25	7647.0
26	7647.0
27	9622.0
28	11597.0
29	13329.5
30	15062.0
31	17316.0
32	19570.0
33	19570.0
34	22604.5
35	25639.0
36	27468.0
37	29297.0
38	30876.0
39	32455.0
40	32455.0
41	33501.0
42	34547.0
43	35691.5
44	36836.0
45	36445.0
46	36054.0
47	36054.0
48	35185.5
49	34317.0
50	34885.5
51	35454.0
52	34418.5
53	33383.0
54	33383.0
55	31615.5
56	29848.0
57	26346.0
58	22844.0
59	21498.0
60	20152.0
61	20152.0
62	18196.5
63	16241.0
64	13445.0
65	10649.0
66	9001.5
67	7354.0
68	7354.0
69	5998.5
70	4643.0
71	3818.5
72	2994.0
73	2344.0
74	1694.0
75	1694.0
76	1262.5
77	831.0
78	644.5
79	458.0
80	368.0
81	278.0
82	278.0
83	226.5
84	175.0
85	159.5
86	144.0
87	111.5
88	79.0
89	79.0
90	60.5
91	42.0
92	25.5
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	489272.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.456179930874534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8641930395665	54.48613683138077
2	7.87593149036383	9.995530515515359
3	2.236548888780589	4.257685461319757
4	1.0888716353997054	2.7638253767019725
5	0.6470965873772282	2.053113874063213
6	0.4172404022539958	1.5885889223916014
7	0.30108263302477634	1.3373887614697173
8	0.20924836110342224	1.0622481321935497
9	0.1668901168616664	0.9531188355832723
>10	1.0687116655059206	12.919643140313259
>50	0.08833119110788855	3.813163421681624
>100	0.03487615260031273	3.7240185982907468
>500	3.259453514047918E-4	0.2057983063204272
>1k	6.518907028095836E-4	0.8397398227748085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2394	0.4892983861737438	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1666	0.3405058944717867	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	995	0.20336336434539481	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.0438529079939174E-4	0.0	0.0
2	0.0	0.0	2.0438529079939174E-4	0.0	0.0
3	0.0	0.0	4.087705815987835E-4	2.0438529079939174E-4	0.0
4	0.0	0.0	6.131558723981753E-4	2.0438529079939174E-4	0.0
5	0.0	0.0	6.131558723981753E-4	2.0438529079939174E-4	0.0
6	0.0	0.0	0.0012263117447963506	2.0438529079939174E-4	0.0
7	0.0	0.0	0.0014306970355957422	4.087705815987835E-4	0.0
8	0.0	0.0	0.0014306970355957422	4.087705815987835E-4	0.0
9	0.0	0.0	0.001635082326395134	0.0010219264539969588	0.0
10	0.0	0.0	0.0018394676171945258	0.0010219264539969588	2.0438529079939174E-4
11	0.0	0.0	0.0020438529079939175	0.0010219264539969588	2.0438529079939174E-4
12	0.0	0.0	0.002248238198793309	0.0010219264539969588	2.0438529079939174E-4
13	0.0	0.0	0.0028613940711914843	0.0010219264539969588	2.0438529079939174E-4
14	0.0	0.0	0.0030657793619908763	0.0012263117447963506	2.0438529079939174E-4
15	0.0	0.0	0.003883320525188443	0.0012263117447963506	2.0438529079939174E-4
16	0.0	0.0	0.004496476397586618	0.001635082326395134	2.0438529079939174E-4
17	0.0	0.0	0.00470086168838601	0.0018394676171945258	2.0438529079939174E-4
18	0.0	0.0	0.00470086168838601	0.0018394676171945258	2.0438529079939174E-4
19	0.0	0.0	0.004905246979185402	0.002248238198793309	2.0438529079939174E-4
20	0.0	0.0	0.0051096322699847934	0.002452623489592701	2.0438529079939174E-4
21	0.0	0.0	0.0053140175607841855	0.0026570087803920927	2.0438529079939174E-4
22	0.0	0.0	0.0055184028515835775	0.0028613940711914843	2.0438529079939174E-4
23	0.0	0.0	0.0055184028515835775	0.0028613940711914843	2.0438529079939174E-4
24	0.0	0.0	0.0055184028515835775	0.0030657793619908763	2.0438529079939174E-4
25	0.0	0.0	0.0055184028515835775	0.0030657793619908763	2.0438529079939174E-4
26	0.0	0.0	0.0055184028515835775	0.0036789352343890515	2.0438529079939174E-4
27	0.0	0.0	0.0055184028515835775	0.004087705815987835	2.0438529079939174E-4
28	2.0438529079939174E-4	0.0	0.0055184028515835775	0.008379796922775061	2.0438529079939174E-4
29	2.0438529079939174E-4	0.0	0.0055184028515835775	0.020438529079939174	2.0438529079939174E-4
30	2.0438529079939174E-4	0.0	0.0055184028515835775	0.03638058176229173	2.0438529079939174E-4
31	2.0438529079939174E-4	0.0	0.0055184028515835775	0.07541817230497555	2.0438529079939174E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATCG	35	8.8620855E-4	26.42857	31
GGTATCA	380	0.0	25.802631	1
GTAATGG	45	1.3216949E-4	24.666668	1
GTATCAA	1030	0.0	24.427183	1
GTCGCCC	75	3.731693E-7	22.2	37
TAACCTG	70	1.21738194E-4	18.5	5
GTTGGCG	50	0.007029249	18.5	9
GTTCTAG	125	8.541974E-9	17.76	1
TATCAAC	1515	0.0	16.240925	2
ATCAACG	1515	0.0	16.240925	3
ATTAGCC	80	3.3776014E-4	16.1875	3
TCTAGAC	115	1.2385553E-6	16.086956	3
CAACGCA	1570	0.0	15.907643	5
TCAACGC	1570	0.0	15.907643	4
GGTCGCC	105	9.321098E-6	15.857142	36
ATAATAT	140	3.4584446E-8	15.857142	3
CGAGCCC	70	0.0025896411	15.857142	17
GCACCGA	95	7.046434E-5	15.578948	13
CTACACT	95	7.046434E-5	15.578948	4
AACGCAG	1640	0.0	15.4542675	6
>>END_MODULE