FastQCFastQC Report
Fri 10 Feb 2017
ERR1633342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852930
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66230.7764998299977723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44660.5236068610554206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23810.27915538203604046No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.13178103713083136No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8620.1010633932444632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.024.6666661
TTAACGG1002.0008883E-1124.05000135
CACGGTA551.901913E-523.5454542
GTATCAA30200.023.4619221
AACGGCC1155.456968E-1222.5217437
ACGGTAC705.0993767E-621.1428593
CGAACTA1551.8189894E-1219.09677529
GCACCGC1409.458745E-1118.510
GTGTCGT500.007033430518.528
TCCGTCG500.007033430518.510
GCGAACT1800.017.47222128
TCACGGT752.0664785E-417.2666661
TCCACGC752.0664785E-417.26666620
CCGGTTA650.001579481817.07692323
TAACGGC1452.9758667E-916.58620636
TATTCCG904.4437944E-516.4444455
TCCGATC803.381286E-416.18758
TATCAAC44350.016.1014652
ATCAACG44000.016.0613653
TCAACGC44700.015.9340044
Produced by FastQC (version 0.11.5)