##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633342.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852930
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88014373981452	33.0	33.0	33.0	33.0	33.0
2	31.979226900214556	33.0	33.0	33.0	33.0	33.0
3	31.700670629477212	33.0	33.0	33.0	27.0	33.0
4	31.974810359584023	33.0	33.0	33.0	33.0	33.0
5	32.07198246045983	33.0	33.0	33.0	33.0	33.0
6	35.235276048444774	37.0	37.0	37.0	33.0	37.0
7	35.3869156906194	37.0	37.0	37.0	33.0	37.0
8	35.51867796888373	37.0	37.0	37.0	33.0	37.0
9	35.639474517252296	37.0	37.0	37.0	33.0	37.0
10	35.63076102376514	37.0	37.0	37.0	33.0	37.0
11	35.650303072936815	37.0	37.0	37.0	33.0	37.0
12	35.657501787954466	37.0	37.0	37.0	33.0	37.0
13	35.660819762465856	37.0	37.0	37.0	33.0	37.0
14	35.627290633463474	37.0	37.0	37.0	33.0	37.0
15	35.651160118649834	37.0	37.0	37.0	33.0	37.0
16	35.644540583635234	37.0	37.0	37.0	33.0	37.0
17	35.626917800991876	37.0	37.0	37.0	33.0	37.0
18	35.61218388378882	37.0	37.0	37.0	33.0	37.0
19	35.59450365211682	37.0	37.0	37.0	33.0	37.0
20	35.60102118579485	37.0	37.0	37.0	33.0	37.0
21	35.60213733835133	37.0	37.0	37.0	33.0	37.0
22	35.50830548814088	37.0	37.0	37.0	33.0	37.0
23	35.55271358728149	37.0	37.0	37.0	33.0	37.0
24	35.55763778973655	37.0	37.0	37.0	33.0	37.0
25	35.57102810312687	37.0	37.0	37.0	33.0	37.0
26	35.46432649807135	37.0	37.0	37.0	33.0	37.0
27	35.47005381449826	37.0	37.0	37.0	33.0	37.0
28	35.49804438816785	37.0	37.0	37.0	33.0	37.0
29	35.50389949937275	37.0	37.0	37.0	33.0	37.0
30	35.49882053626909	37.0	37.0	37.0	33.0	37.0
31	35.49263480004221	37.0	37.0	37.0	33.0	37.0
32	35.47303295698357	37.0	37.0	37.0	33.0	37.0
33	35.47012650510593	37.0	37.0	37.0	33.0	37.0
34	35.43297105272414	37.0	37.0	37.0	33.0	37.0
35	35.373294408685354	37.0	37.0	37.0	33.0	37.0
36	35.394956209771024	37.0	37.0	37.0	33.0	37.0
37	35.390489254686784	37.0	37.0	37.0	33.0	37.0
38	35.3702578171714	37.0	37.0	37.0	33.0	37.0
39	35.27076782385424	37.0	37.0	37.0	33.0	37.0
40	35.04250876390794	37.0	37.0	37.0	27.0	37.0
41	35.197777074320285	37.0	37.0	37.0	33.0	37.0
42	35.23480824921154	37.0	37.0	37.0	33.0	37.0
43	34.89082808671286	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	28.0
16	44.0
17	49.0
18	46.0
19	43.0
20	66.0
21	179.0
22	365.0
23	862.0
24	1832.0
25	3275.0
26	5474.0
27	8289.0
28	11720.0
29	15683.0
30	20787.0
31	27214.0
32	34558.0
33	45224.0
34	65623.0
35	131986.0
36	479582.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.8612312850996	20.339535483568405	14.33411886086783	23.465114370464164
2	15.560479758010622	22.399141781857832	39.7968180272707	22.243560432860846
3	17.732522012357403	28.077450670043262	30.802645000175865	23.38738231742347
4	12.08493076805834	18.137948014491222	40.40870880377053	29.368412413679902
5	13.425017293330049	37.290047248894986	35.22364086150095	14.06129459627402
6	28.125989237100345	40.836176474036556	17.54716096279882	13.490673326064273
7	25.5427760777555	33.174938154362025	23.614247359103327	17.66803840877915
8	24.3980162498681	35.49599615443237	20.847314551018254	19.258673044681277
9	26.365117887751634	15.38625678543374	20.49523407548099	37.75339125133364
10	14.81200098484049	28.278639513207416	33.7923393478949	23.11702015405719
11	32.89308618526726	24.498610671450177	24.338105119998126	18.270198023284443
12	22.930721161173835	26.22923334857491	30.680008910461588	20.16003657978967
13	28.16807944379961	22.92134172792609	26.584713868664483	22.325864959609813
14	22.248015663653522	21.583717303881915	28.831088131499655	27.337178900964908
15	23.66876531485585	28.656396187260384	25.735523430996682	21.939315066887083
16	21.92090792913838	29.154444092715696	25.72450259693058	23.20014538121534
17	21.960536034610108	27.409517779888148	26.951215222820164	23.67873096268158
18	23.175993340602393	26.546844406926713	28.56940194388754	21.707760308583353
19	24.77026250688802	26.09592815354132	28.760859625057154	20.3729497145135
20	24.519010938764023	25.844442099586136	28.55920181023062	21.077345151419223
21	22.700690560772866	27.07572719918399	28.347343861747156	21.87623837829599
22	22.23078095506079	27.06880986716378	28.556270737340693	22.144138440434737
23	22.0864549259611	26.757178197507415	29.093243290774158	22.06312358575733
24	22.44428030436261	27.005264206910297	28.41100676491623	22.139448723810865
25	22.5701991957136	26.76479898702121	28.947041375024913	21.717960442240276
26	22.545695426353863	26.866917566506043	28.555567279847118	22.031819727292977
27	22.595054693820128	26.852027716225248	28.33362644062233	22.219291149332303
28	21.933804649854032	26.929525283434746	28.736121369866225	22.400548696844993
29	22.631634483486334	26.9535600811321	28.278170541545027	22.13663489383654
30	22.604199641236676	26.89599380957406	28.712203815084475	21.78760273410479
31	22.514508810805108	26.85859331949867	28.554277607775553	22.072620261920672
32	21.60083476955905	26.80395812083055	28.879626698556738	22.71558041105366
33	21.771657697583624	26.69046697853282	29.334646453988018	22.203228869895536
34	22.18458724631564	26.506864572708196	28.77070802996729	22.537840151008876
35	22.056089010821523	27.334599556821782	28.489559518366104	22.119751913990598
36	22.667862544405754	26.946877234943077	28.46165570445406	21.92360451619711
37	22.85967195432216	26.743109047635798	28.12223746380125	22.274981534240794
38	22.636675928857	26.99295370077263	28.205128205128204	22.165242165242166
39	22.269002145545354	26.771950804872613	28.398227287116175	22.560819762465854
40	22.2267946959305	27.18019063698076	28.580774506700436	22.01224016038831
41	22.288816198281218	26.593389844418653	28.510897729004725	22.606896228295405
42	21.952211787602735	27.098472324809777	28.41464129529973	22.53467459228776
43	22.229608525904823	26.639818038994996	28.43234497555485	22.698228459545334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	53.5
2	91.0
3	329.5
4	568.0
5	568.0
6	735.0
7	902.0
8	822.0
9	742.0
10	1074.5
11	1407.0
12	1407.0
13	2243.0
14	3079.0
15	5747.0
16	8415.0
17	9683.5
18	10952.0
19	10952.0
20	9917.0
21	8882.0
22	9582.5
23	10283.0
24	12796.5
25	15310.0
26	15310.0
27	18671.5
28	22033.0
29	26357.0
30	30681.0
31	34077.5
32	37474.0
33	37474.0
34	41578.0
35	45682.0
36	48603.0
37	51524.0
38	54045.5
39	56567.0
40	56567.0
41	58498.0
42	60429.0
43	59761.5
44	59094.0
45	59938.0
46	60782.0
47	60782.0
48	60011.5
49	59241.0
50	60475.5
51	61710.0
52	61053.5
53	60397.0
54	60397.0
55	55209.5
56	50022.0
57	44917.5
58	39813.0
59	35303.0
60	30793.0
61	30793.0
62	26890.0
63	22987.0
64	18955.5
65	14924.0
66	12670.5
67	10417.0
68	10417.0
69	8499.0
70	6581.0
71	5661.5
72	4742.0
73	3824.5
74	2907.0
75	2907.0
76	2163.5
77	1420.0
78	1112.0
79	804.0
80	631.5
81	459.0
82	459.0
83	381.0
84	303.0
85	269.5
86	236.0
87	195.0
88	154.0
89	154.0
90	119.5
91	85.0
92	51.5
93	18.0
94	11.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	852930.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.413779148938495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.09371368337494	54.982615902439846
2	7.270351343967772	9.075402062351854
3	1.6539869320706877	3.096947252804707
4	0.7372532197334369	1.8405903853314625
5	0.4307599403897554	1.3442677892848052
6	0.25465252301867375	0.9536295798844489
7	0.18619464512185366	0.8134778021545247
8	0.15902527939466543	0.794029493378951
9	0.11760332953078872	0.6606061412863036
>10	0.9095667492623152	11.605026264305273
>50	0.11977977062566408	5.147546017373967
>100	0.0650212565495499	7.418952551729406
>500	0.0013308444290276017	0.5351632379141524
>1k	5.703618981546865E-4	0.9458505918877403
>5k	1.901206327182288E-4	0.7858949278726013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6623	0.7764998299977723	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4466	0.5236068610554206	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2381	0.27915538203604046	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.13178103713083136	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	862	0.1010633932444632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.344858311936501E-4	0.0	0.0
2	0.0	0.0	3.517287467904752E-4	0.0	0.0
3	0.0	0.0	4.689716623873002E-4	0.0	0.0
4	0.0	0.0	5.862145779841253E-4	0.0	0.0
5	1.1724291559682506E-4	0.0	7.034574935809504E-4	0.0	0.0
6	1.1724291559682506E-4	0.0	0.0018758866495492009	1.1724291559682506E-4	0.0
7	1.1724291559682506E-4	0.0	0.004103502045888877	2.344858311936501E-4	0.0
8	1.1724291559682506E-4	0.0	0.004806959539469828	2.344858311936501E-4	0.0
9	1.1724291559682506E-4	0.0	0.006213874526631728	5.862145779841253E-4	0.0
10	2.344858311936501E-4	0.0	0.007386303682599979	7.034574935809504E-4	0.0
11	2.344858311936501E-4	0.0	0.010317376572520605	9.379433247746004E-4	0.0
12	2.344858311936501E-4	0.0	0.01184153447527933	9.379433247746004E-4	0.0
13	2.344858311936501E-4	0.0	0.012779477800053932	0.0011724291559682507	0.0
14	2.344858311936501E-4	0.0	0.013482935293634882	0.0011724291559682507	0.0
15	3.517287467904752E-4	0.0	0.014772607365199957	0.0012896720715650757	0.0
16	3.517287467904752E-4	0.0	0.01594503652116821	0.001641400818355551	0.0
17	3.517287467904752E-4	0.0	0.01617952235236186	0.0018758866495492009	0.0
18	5.862145779841253E-4	0.0	0.016882979845942808	0.0018758866495492009	0.0
19	5.862145779841253E-4	0.0	0.017000222761539632	0.0018758866495492009	0.0
20	5.862145779841253E-4	0.0	0.017234708592733284	0.0018758866495492009	0.0
21	5.862145779841253E-4	0.0	0.01828989483310471	0.0023448583119365014	0.0
22	5.862145779841253E-4	0.0	0.018876109411088835	0.0024621012275333263	0.0
23	5.862145779841253E-4	0.0	0.018876109411088835	0.0030483158055174516	0.0
24	5.862145779841253E-4	0.0	0.01899335232668566	0.003986259130292052	0.0
25	5.862145779841253E-4	0.0	0.01899335232668566	0.004572473708276178	0.0
26	5.862145779841253E-4	0.0	0.01899335232668566	0.005627659948647603	0.0
27	8.207004091777755E-4	0.0	0.019110595242282487	0.006682846189019029	0.0
28	8.207004091777755E-4	0.0	0.019110595242282487	0.015007093196393607	0.0
29	8.207004091777755E-4	0.0	0.019110595242282487	0.03634530383501577	0.0
30	8.207004091777755E-4	0.0	0.019110595242282487	0.06647673314339982	0.0
31	8.207004091777755E-4	0.0	0.019110595242282487	0.12603613426658694	1.1724291559682506E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1065	0.0	24.666666	1
TTAACGG	100	2.0008883E-11	24.050001	35
CACGGTA	55	1.901913E-5	23.545454	2
GTATCAA	3020	0.0	23.461922	1
AACGGCC	115	5.456968E-12	22.52174	37
ACGGTAC	70	5.0993767E-6	21.142859	3
CGAACTA	155	1.8189894E-12	19.096775	29
GCACCGC	140	9.458745E-11	18.5	10
GTGTCGT	50	0.0070334305	18.5	28
TCCGTCG	50	0.0070334305	18.5	10
GCGAACT	180	0.0	17.472221	28
TCACGGT	75	2.0664785E-4	17.266666	1
TCCACGC	75	2.0664785E-4	17.266666	20
CCGGTTA	65	0.0015794818	17.076923	23
TAACGGC	145	2.9758667E-9	16.586206	36
TATTCCG	90	4.4437944E-5	16.444445	5
TCCGATC	80	3.381286E-4	16.1875	8
TATCAAC	4435	0.0	16.101465	2
ATCAACG	4400	0.0	16.061365	3
TCAACGC	4470	0.0	15.934004	4
>>END_MODULE