FastQCFastQC Report
Fri 10 Feb 2017
ERR1633341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840156
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27920.33231923595141855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19990.23793200310418544No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18840.22424406895862198No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15550.18508467475087959No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG14560.17330114883426412No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC14530.17294407229133638No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13220.15735172991682497No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT12260.14592528054313722No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11740.13973595379905637No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC11150.13271344845481078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11050.1315231933117183No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10810.12866658096829636No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10340.12307238179576174No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10320.12283433076714324No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10300.12259627973852476No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10100.12021576945233982No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8910.10605173324953938No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8850.10533758016368387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6550.024.2900751
GTATCAA18100.023.5082871
TCTATAC806.9555244E-720.81253
CGTGCGC450.003824964620.55555531
CGCTTTA450.003824964620.55555535
GTATATC1101.7535058E-920.1818181
TCACGTA1700.019.58823625
CGCCTAT500.00703334318.536
TTACGGC500.00703334318.518
TCTATCG500.00703334318.531
GTTTAGG1552.0008883E-1117.9032271
TATTAGA2450.017.3673482
CGAAATT752.0664326E-417.26666625
CCGTCCG752.0664326E-417.26666628
TAGTACC1502.5102054E-1017.2666664
TTAACGG2050.017.14634335
CCGTTTA650.001579455317.07692327
GTCCTAG1201.0401891E-716.9583321
ATCACGT1757.2759576E-1216.91428624
CGGCCTT2750.016.81818224
Produced by FastQC (version 0.11.5)