##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633341.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840156
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83023628945101	33.0	33.0	33.0	27.0	33.0
2	31.94892139078933	33.0	33.0	33.0	33.0	33.0
3	31.656384052485492	33.0	33.0	33.0	27.0	33.0
4	31.931350844367	33.0	33.0	33.0	33.0	33.0
5	32.03528392346183	33.0	33.0	33.0	33.0	33.0
6	35.18573693456929	37.0	37.0	37.0	33.0	37.0
7	35.3483472117083	37.0	37.0	37.0	33.0	37.0
8	35.47400125690943	37.0	37.0	37.0	33.0	37.0
9	35.596027404434416	37.0	37.0	37.0	33.0	37.0
10	35.599498188431674	37.0	37.0	37.0	33.0	37.0
11	35.62216421712158	37.0	37.0	37.0	33.0	37.0
12	35.61546308066597	37.0	37.0	37.0	33.0	37.0
13	35.62949380829274	37.0	37.0	37.0	33.0	37.0
14	35.594409847694955	37.0	37.0	37.0	33.0	37.0
15	35.60745623431839	37.0	37.0	37.0	33.0	37.0
16	35.59137469708006	37.0	37.0	37.0	33.0	37.0
17	35.58885730745243	37.0	37.0	37.0	33.0	37.0
18	35.567449378448764	37.0	37.0	37.0	33.0	37.0
19	35.565216459800325	37.0	37.0	37.0	33.0	37.0
20	35.56679235760978	37.0	37.0	37.0	33.0	37.0
21	35.568757468851025	37.0	37.0	37.0	33.0	37.0
22	35.47678526368912	37.0	37.0	37.0	33.0	37.0
23	35.52505725127238	37.0	37.0	37.0	33.0	37.0
24	35.51070277424669	37.0	37.0	37.0	33.0	37.0
25	35.527624631616035	37.0	37.0	37.0	33.0	37.0
26	35.415488314075006	37.0	37.0	37.0	33.0	37.0
27	35.446640861935165	37.0	37.0	37.0	33.0	37.0
28	35.46157737372583	37.0	37.0	37.0	33.0	37.0
29	35.472228967001364	37.0	37.0	37.0	33.0	37.0
30	35.46737867729326	37.0	37.0	37.0	33.0	37.0
31	35.46591466346726	37.0	37.0	37.0	33.0	37.0
32	35.4627033550912	37.0	37.0	37.0	33.0	37.0
33	35.45701274525207	37.0	37.0	37.0	33.0	37.0
34	35.43875780212246	37.0	37.0	37.0	33.0	37.0
35	35.38406795880765	37.0	37.0	37.0	33.0	37.0
36	35.41050233528059	37.0	37.0	37.0	33.0	37.0
37	35.42192521388885	37.0	37.0	37.0	33.0	37.0
38	35.39924728264751	37.0	37.0	37.0	33.0	37.0
39	35.32043929936821	37.0	37.0	37.0	33.0	37.0
40	35.112118463713884	37.0	37.0	37.0	33.0	37.0
41	35.28356400478007	37.0	37.0	37.0	33.0	37.0
42	35.335867386532975	37.0	37.0	37.0	33.0	37.0
43	34.99217050166874	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	29.0
16	43.0
17	45.0
18	40.0
19	31.0
20	56.0
21	169.0
22	384.0
23	936.0
24	1869.0
25	3421.0
26	5456.0
27	8338.0
28	11903.0
29	15907.0
30	20674.0
31	26332.0
32	33972.0
33	44603.0
34	64427.0
35	132294.0
36	469223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.980246525645235	20.70056037212137	14.024538300029995	24.2946548022034
2	15.758859069030038	21.804998119396874	39.47409766757602	22.962045143997067
3	17.80443155794876	27.235418184241972	30.922590566513836	24.037559691295428
4	12.76346297592352	18.18745566299592	38.8405248549079	30.208556506172663
5	13.455358290603172	37.817262508391295	35.49483667318927	13.232542527816262
6	29.241355176895716	39.517303929270284	17.769080980198915	13.472259913635087
7	26.03445074486167	32.62870228862259	23.6446564685606	17.69219049795514
8	23.860806802546193	36.64795585581725	20.664971743342903	18.826265598293652
9	25.363980022757676	16.492770390260855	20.175300777474657	37.96794880950681
10	14.773089759520852	28.635396283547344	33.78658249182295	22.80493146510886
11	32.79509995762692	24.2135984269588	24.505210937016457	18.486090678397822
12	22.46642290241336	26.38248134870191	29.790300848889967	21.36079489999476
13	27.099014944843574	22.517722899080646	27.041287570403593	23.341974585672183
14	21.804760068368257	21.578492565666377	27.523340903356043	29.093406462609323
15	23.933769442817763	28.863092092420935	25.00964106665905	22.19349739810226
16	22.994777160432108	29.232547288836834	24.56972276577207	23.20295278495898
17	22.492727541075705	28.047648293888276	26.053494827151148	23.406129337884867
18	22.20075795447512	27.978375444560292	27.791743438123394	22.029123162841184
19	24.898471236294213	26.835373430648595	27.593327905769883	20.672827427287313
20	24.242045524878712	26.10396164521827	28.334023681316328	21.31996914858669
21	22.206828255704895	27.911602131032808	27.156980370312176	22.72458924295012
22	22.13029485000405	27.717828593737355	26.992368084022488	23.15950847223611
23	22.06435471507672	27.75627383485924	27.5935659567985	22.585805493265536
24	23.480758335356768	27.99182532767724	26.311423116659288	22.215993220306704
25	23.308171339608357	26.991772956450944	27.3542056475226	22.345850056418094
26	22.884916610724677	27.322306809687724	27.29612119653969	22.49665538304791
27	22.72958831455111	27.03831193254586	26.970586414903895	23.261513337999133
28	21.844633615661856	27.421217012078706	27.913030437204515	22.82111893505492
29	23.48313884564295	27.597136722227777	26.678616828303316	22.241107603825956
30	22.400720818514657	27.769723717976184	27.267197996562544	22.562357466946615
31	22.9483572098515	27.20244811677831	27.497036264693698	22.352158408676484
32	21.911168878160723	27.16995415137189	27.6128480901166	23.306028880350794
33	22.013649845980986	26.981536762220347	28.009560129309317	22.99525326248935
34	22.386556782311857	27.109013088045554	27.554882664647995	22.949547464994595
35	22.872180880693584	27.643199596265454	26.973085950704395	22.511533572336567
36	23.2211636886483	27.62367941191874	26.700874599479146	22.454282299953817
37	23.488971095844104	26.4917467708378	26.934402658553886	23.08487947476421
38	23.419341169973194	27.27600588462143	26.957612633844192	22.347040311561187
39	22.61568089735716	27.12222492013388	27.388604021157974	22.873490161350986
40	22.874442365465463	27.157337446855106	27.42300239479335	22.54521779288608
41	22.359895067106585	27.044739310318562	27.701641123791298	22.89372449878356
42	22.865277400863647	27.097229562128938	27.070567846923666	22.966925190083746
43	22.753750493955884	26.613747923004777	27.227086398240324	23.405415184799015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	29.0
2	40.0
3	140.0
4	240.0
5	240.0
6	302.5
7	365.0
8	357.5
9	350.0
10	506.0
11	662.0
12	662.0
13	1001.5
14	1341.0
15	2867.0
16	4393.0
17	4913.0
18	5433.0
19	5433.0
20	5298.5
21	5164.0
22	6085.0
23	7006.0
24	8964.0
25	10922.0
26	10922.0
27	14239.5
28	17557.0
29	23110.5
30	28664.0
31	31471.5
32	34279.0
33	34279.0
34	39892.0
35	45505.0
36	48324.5
37	51144.0
38	57475.5
39	63807.0
40	63807.0
41	67952.0
42	72097.0
43	67632.0
44	63167.0
45	64156.0
46	65145.0
47	65145.0
48	64100.5
49	63056.0
50	65012.5
51	66969.0
52	66124.5
53	65280.0
54	65280.0
55	56306.5
56	47333.0
57	40630.5
58	33928.0
59	29895.0
60	25862.0
61	25862.0
62	22994.0
63	20126.0
64	16683.5
65	13241.0
66	11325.0
67	9409.0
68	9409.0
69	7885.5
70	6362.0
71	5413.0
72	4464.0
73	3634.0
74	2804.0
75	2804.0
76	2188.0
77	1572.0
78	1298.5
79	1025.0
80	781.5
81	538.0
82	538.0
83	440.0
84	342.0
85	304.5
86	267.0
87	222.0
88	177.0
89	177.0
90	129.0
91	81.0
92	49.0
93	17.0
94	10.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	840156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63808499426739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.79616868340021	42.58757513289122
2	7.966572023446491	7.908907584255793
3	2.18026111322905	3.246719593444789
4	0.9623841836188686	1.9108363161444808
5	0.5579282148454999	1.38472440746004
6	0.3804864532238356	1.1331971342575256
7	0.26750636768666114	0.929495267101676
8	0.211054740192197	0.8381082505682637
9	0.16182800718688783	0.7229549137676087
>10	1.1603046451511658	11.671311313703152
>50	0.18177891987071912	6.3430953607700165
>100	0.16055075067658367	15.631713478131859
>500	0.009271927850550494	2.9943504952444346
>1k	0.003903969621284418	2.697010752259154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2792	0.33231923595141855	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1999	0.23793200310418544	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1884	0.22424406895862198	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1555	0.18508467475087959	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1456	0.17330114883426412	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1453	0.17294407229133638	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1322	0.15735172991682497	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1226	0.14592528054313722	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1174	0.13973595379905637	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1115	0.13271344845481078	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1105	0.1315231933117183	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1081	0.12866658096829636	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1034	0.12307238179576174	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1032	0.12283433076714324	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1030	0.12259627973852476	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1010	0.12021576945233982	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	891	0.10605173324953938	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	885	0.10533758016368387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.1902551430924732E-4	0.0	0.0	0.0
4	0.0	1.1902551430924732E-4	1.1902551430924732E-4	0.0	0.0
5	0.0	1.1902551430924732E-4	2.3805102861849465E-4	0.0	0.0
6	0.0	1.1902551430924732E-4	8.331786001647313E-4	0.0	0.0
7	0.0	1.1902551430924732E-4	0.0027375868291126884	0.0	0.0
8	0.0	1.1902551430924732E-4	0.003213688886349678	0.0	0.0
9	0.0	1.1902551430924732E-4	0.0036897909435866672	0.0	0.0
10	1.1902551430924732E-4	1.1902551430924732E-4	0.0040468674865144095	0.0	0.0
11	1.1902551430924732E-4	1.1902551430924732E-4	0.005713224686843872	0.0	0.0
12	1.1902551430924732E-4	1.1902551430924732E-4	0.005832250201153119	0.0	0.0
13	2.3805102861849465E-4	1.1902551430924732E-4	0.006070301229771614	0.0	0.0
14	2.3805102861849465E-4	1.1902551430924732E-4	0.006189326744080861	0.0	0.0
15	2.3805102861849465E-4	1.1902551430924732E-4	0.006427377772699356	1.1902551430924732E-4	0.0
16	2.3805102861849465E-4	1.1902551430924732E-4	0.006546403287008603	1.1902551430924732E-4	0.0
17	2.3805102861849465E-4	1.1902551430924732E-4	0.006784454315627098	4.761020572369893E-4	0.0
18	2.3805102861849465E-4	1.1902551430924732E-4	0.006784454315627098	5.951275715462366E-4	1.1902551430924732E-4
19	2.3805102861849465E-4	1.1902551430924732E-4	0.006903479829936345	7.14153085855484E-4	1.1902551430924732E-4
20	2.3805102861849465E-4	1.1902551430924732E-4	0.00714153085855484	0.001071229628783226	1.1902551430924732E-4
21	2.3805102861849465E-4	1.1902551430924732E-4	0.0073795818871733344	0.0013092806574017206	1.1902551430924732E-4
22	2.3805102861849465E-4	1.1902551430924732E-4	0.007498607401482582	0.001428306171710968	1.1902551430924732E-4
23	2.3805102861849465E-4	1.1902551430924732E-4	0.007498607401482582	0.0015473316860202153	1.1902551430924732E-4
24	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.0016663572003294627	1.1902551430924732E-4
25	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.00178538271463871	1.1902551430924732E-4
26	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.0019044082289479572	2.3805102861849465E-4
27	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.002856612343421936	2.3805102861849465E-4
28	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.01023619423059527	2.3805102861849465E-4
29	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.02047238846119054	2.3805102861849465E-4
30	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.039397445236360865	2.3805102861849465E-4
31	2.3805102861849465E-4	1.1902551430924732E-4	0.007617632915791829	0.08879303367469851	2.3805102861849465E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	655	0.0	24.290075	1
GTATCAA	1810	0.0	23.508287	1
TCTATAC	80	6.9555244E-7	20.8125	3
CGTGCGC	45	0.0038249646	20.555555	31
CGCTTTA	45	0.0038249646	20.555555	35
GTATATC	110	1.7535058E-9	20.181818	1
TCACGTA	170	0.0	19.588236	25
CGCCTAT	50	0.007033343	18.5	36
TTACGGC	50	0.007033343	18.5	18
TCTATCG	50	0.007033343	18.5	31
GTTTAGG	155	2.0008883E-11	17.903227	1
TATTAGA	245	0.0	17.367348	2
CGAAATT	75	2.0664326E-4	17.266666	25
CCGTCCG	75	2.0664326E-4	17.266666	28
TAGTACC	150	2.5102054E-10	17.266666	4
TTAACGG	205	0.0	17.146343	35
CCGTTTA	65	0.0015794553	17.076923	27
GTCCTAG	120	1.0401891E-7	16.958332	1
ATCACGT	175	7.2759576E-12	16.914286	24
CGGCCTT	275	0.0	16.818182	24
>>END_MODULE