FastQCFastQC Report
Fri 10 Feb 2017
ERR1633340.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633340.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878470
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49080.5586986465104101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.38225551242501166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19790.22527804022903458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9400.025.7819161
CGCTCTC1650.023.54545429
GTATCAA26000.023.4096151
CACGGTA802.7239366E-823.1250022
CCGCTCT1750.022.228
GCTTCGC2200.021.02272822
ATAGGAC1151.3460522E-1020.9130443
GCCGCTC1900.020.44736927
ACGGTAC1001.288754E-820.353
GGCCTAG656.8997644E-519.9230771
TTCGCCG2050.019.8536624
GCCGGCA2150.019.79069715
CCGGCAG2350.018.89361816
AGCTTCG2350.018.89361821
CGGCAGC2400.018.517
TTAACGG1409.458745E-1118.535
GTATCAC500.007033593518.51
CGAACTA1552.0008883E-1117.90322529
CTTCGCC2600.017.78846223
TCACGGT1054.794074E-717.6190471
Produced by FastQC (version 0.11.5)