##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633340.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	878470
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91309777226314	33.0	33.0	33.0	33.0	33.0
2	32.02617050098467	33.0	33.0	33.0	33.0	33.0
3	31.725650278324814	33.0	33.0	33.0	27.0	33.0
4	32.000711464250344	33.0	33.0	33.0	33.0	33.0
5	32.10912837091762	33.0	33.0	33.0	33.0	33.0
6	35.284241920611976	37.0	37.0	37.0	33.0	37.0
7	35.42746024337769	37.0	37.0	37.0	33.0	37.0
8	35.56753901669949	37.0	37.0	37.0	33.0	37.0
9	35.67077760196705	37.0	37.0	37.0	33.0	37.0
10	35.66954705339966	37.0	37.0	37.0	33.0	37.0
11	35.70174963288445	37.0	37.0	37.0	33.0	37.0
12	35.70244288364998	37.0	37.0	37.0	33.0	37.0
13	35.70306441881908	37.0	37.0	37.0	33.0	37.0
14	35.6692135189591	37.0	37.0	37.0	33.0	37.0
15	35.69573349118353	37.0	37.0	37.0	33.0	37.0
16	35.68802691042381	37.0	37.0	37.0	33.0	37.0
17	35.6781028378886	37.0	37.0	37.0	33.0	37.0
18	35.651209489225586	37.0	37.0	37.0	33.0	37.0
19	35.655052534520244	37.0	37.0	37.0	33.0	37.0
20	35.65352943185311	37.0	37.0	37.0	33.0	37.0
21	35.64977859232529	37.0	37.0	37.0	33.0	37.0
22	35.55985292611017	37.0	37.0	37.0	33.0	37.0
23	35.605158969572095	37.0	37.0	37.0	33.0	37.0
24	35.598171821462316	37.0	37.0	37.0	33.0	37.0
25	35.621625098182065	37.0	37.0	37.0	33.0	37.0
26	35.513536034241355	37.0	37.0	37.0	33.0	37.0
27	35.526870581807	37.0	37.0	37.0	33.0	37.0
28	35.55272234680752	37.0	37.0	37.0	33.0	37.0
29	35.55835145195624	37.0	37.0	37.0	33.0	37.0
30	35.55979031725614	37.0	37.0	37.0	33.0	37.0
31	35.55071886347855	37.0	37.0	37.0	33.0	37.0
32	35.535274966703476	37.0	37.0	37.0	33.0	37.0
33	35.52982571971724	37.0	37.0	37.0	33.0	37.0
34	35.50354707616652	37.0	37.0	37.0	33.0	37.0
35	35.44702835611916	37.0	37.0	37.0	33.0	37.0
36	35.477320796384625	37.0	37.0	37.0	33.0	37.0
37	35.47628490443612	37.0	37.0	37.0	33.0	37.0
38	35.446022061083475	37.0	37.0	37.0	33.0	37.0
39	35.35199836078637	37.0	37.0	37.0	33.0	37.0
40	35.15028970824274	37.0	37.0	37.0	33.0	37.0
41	35.31066171866996	37.0	37.0	37.0	33.0	37.0
42	35.34618484410395	37.0	37.0	37.0	33.0	37.0
43	35.01377963960067	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	30.0
16	58.0
17	46.0
18	35.0
19	25.0
20	48.0
21	129.0
22	328.0
23	785.0
24	1688.0
25	3049.0
26	5135.0
27	8147.0
28	11275.0
29	15788.0
30	20712.0
31	26733.0
32	34154.0
33	45382.0
34	65967.0
35	135050.0
36	503900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.457761790385554	20.367798558858013	14.155065056291052	24.01937459446538
2	15.602695595751703	22.129611711270734	39.62571288717884	22.64197980579872
3	17.726274090179515	27.605268250480954	30.78226917253862	23.886188486800915
4	12.356483431420537	18.129247441574556	40.148326066911785	29.365943060093112
5	13.47012419319954	37.451705806686626	35.423975776065205	13.654194224048629
6	28.521975707764636	40.38111716962446	17.686432092160235	13.41047503045067
7	25.68681912871242	32.995776748209956	23.669675686136124	17.647728436941502
8	24.236001229410224	35.90845447197969	20.95518344394231	18.900360854667774
9	26.161621910822223	15.698999396678314	20.44292918369438	37.69644950880508
10	14.68132093298576	28.476897332862816	34.04225528475645	22.79952644939497
11	32.97050553803773	24.329231504775347	24.47049984632372	18.229763110863207
12	22.622741812469407	26.192015663596933	30.63086958006534	20.554372943868316
13	27.834188987671748	22.770612542260977	26.805013261693627	22.59018520837365
14	21.95180256582467	21.747014696005554	28.475303652942046	27.82587908522773
15	23.63211037371794	28.966157068539623	25.217366557765203	22.18436599997723
16	22.15306157296208	29.296276480699397	25.60019124159049	22.950470704748028
17	21.94053297209922	27.882340888135055	26.84246473983175	23.334661399933974
18	22.786890844308854	27.159607044065247	28.290550616412624	21.76295149521327
19	24.454221544275843	26.595216683552085	28.418272678634445	20.532289093537628
20	24.28301478707298	26.177217207189774	28.410531947590695	21.12923605814655
21	22.653477068084282	27.271733809919517	27.99105262558767	22.08373649640853
22	22.165014172367865	27.26353774175555	28.057759513699953	22.513688572176623
23	22.142930322037177	27.104966589638803	28.608034423486288	22.14406866483773
24	22.607032681821803	27.32193472742382	27.872209637210148	22.19882295354423
25	22.553758238756018	27.016972691156216	28.51104761687935	21.91822145320842
26	22.589160699853153	27.234965337461723	28.08268922103202	22.0931847416531
27	22.597925939417397	27.040422552847566	27.980693706102656	22.380957801632384
28	21.95931562830831	27.170193632110372	28.399490022425354	22.471000717155963
29	22.919052443452824	27.22972896057919	27.814267988662106	22.036950607305883
30	22.499003950049516	27.21208464717065	28.326863751750203	21.962047651029632
31	22.66770635309117	27.05055380377247	28.16624358259246	22.115496260543903
32	21.615991439662142	27.118399034685304	28.44411306020695	22.821496465445605
33	21.894885425797124	26.866483772923377	28.932803624483476	22.30582717679602
34	22.136783270914204	26.93239382107528	28.417248170113947	22.51357473789657
35	22.261090304734367	27.479822873860233	28.106025248443316	22.15306157296208
36	22.714947579314035	27.15676118706387	28.028845606565962	22.099445627056134
37	22.88979703347866	26.69106514735848	27.911710132389267	22.507427686773596
38	22.758090771454917	27.113959497763158	27.844547907156763	22.283401823625166
39	22.33622092957073	26.965291928011204	28.013819481598688	22.68466766081938
40	22.29626509727139	27.306225596776212	28.31491115234442	22.082598153607975
41	22.21601192983255	26.866142270083216	28.330278780151858	22.587567019932383
42	22.089997381811557	27.193415825241612	28.197206506767447	22.51938028617938
43	22.36149213974296	26.709620135007455	28.08337222671235	22.84551549853723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	45.0
2	62.0
3	257.0
4	452.0
5	452.0
6	555.5
7	659.0
8	633.5
9	608.0
10	857.0
11	1106.0
12	1106.0
13	1739.0
14	2372.0
15	4518.5
16	6665.0
17	7725.5
18	8786.0
19	8786.0
20	8497.5
21	8209.0
22	9166.5
23	10124.0
24	12863.5
25	15603.0
26	15603.0
27	19289.5
28	22976.0
29	27521.5
30	32067.0
31	36006.0
32	39945.0
33	39945.0
34	44251.5
35	48558.0
36	51614.0
37	54670.0
38	57534.5
39	60399.0
40	60399.0
41	62535.0
42	64671.0
43	63385.0
44	62099.0
45	62718.0
46	63337.0
47	63337.0
48	62464.0
49	61591.0
50	62947.5
51	64304.0
52	63362.0
53	62420.0
54	62420.0
55	56259.0
56	50098.0
57	44884.5
58	39671.0
59	35067.5
60	30464.0
61	30464.0
62	26602.0
63	22740.0
64	18984.0
65	15228.0
66	12901.0
67	10574.0
68	10574.0
69	8618.5
70	6663.0
71	5753.5
72	4844.0
73	3912.5
74	2981.0
75	2981.0
76	2212.5
77	1444.0
78	1129.5
79	815.0
80	642.0
81	469.0
82	469.0
83	392.5
84	316.0
85	272.5
86	229.0
87	188.5
88	148.0
89	148.0
90	101.5
91	55.0
92	36.0
93	17.0
94	10.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	878470.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.308146889686654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.00984761386468	54.837305128636196
2	7.374731622676681	9.190117224355115
3	1.6918058524290034	3.162398626859337
4	0.714497518636992	1.7807606537420129
5	0.4260051479061784	1.3271795665750425
6	0.2761932781046531	1.0325454805253282
7	0.19593597285638945	0.8545885164396699
8	0.13253997730329162	0.6606656299655383
9	0.11146306747727076	0.6250551463033958
>10	0.8671713718003259	11.014844155108976
>50	0.12395717056353961	5.3720840370574
>100	0.0730763549399901	8.144212099377333
>500	0.0022200411527862557	0.8172799585542444
>1k	5.550102881965639E-4	1.1809637765004561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4908	0.5586986465104101	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3358	0.38225551242501166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1979	0.22527804022903458	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.4150284016528737E-4	0.0	0.0
2	0.0	0.0	3.4150284016528737E-4	0.0	0.0
3	0.0	0.0	4.553371202203832E-4	0.0	0.0
4	0.0	0.0	6.830056803305747E-4	1.138342800550958E-4	0.0
5	0.0	0.0	9.106742404407664E-4	1.138342800550958E-4	0.0
6	0.0	0.0	0.001593679920771341	1.138342800550958E-4	0.0
7	0.0	0.0	0.0036426969617630654	1.138342800550958E-4	0.0
8	0.0	0.0	0.004098034081983448	1.138342800550958E-4	0.0
9	0.0	0.0	0.005350211162589502	0.001024508520495862	0.0
10	1.138342800550958E-4	0.0	0.00648855396314046	0.0011383428005509579	0.0
11	1.138342800550958E-4	0.0	0.010358919485013718	0.001593679920771341	0.0
12	1.138342800550958E-4	0.0	0.01138342800550958	0.001593679920771341	0.0
13	1.138342800550958E-4	0.0	0.012294102245950345	0.001593679920771341	0.0
14	1.138342800550958E-4	0.0	0.013432445046501304	0.001707514200826437	0.0
15	1.138342800550958E-4	0.0	0.014456953566997166	0.001707514200826437	0.0
16	1.138342800550958E-4	0.0	0.015481462087493028	0.001707514200826437	0.0
17	1.138342800550958E-4	0.0	0.015481462087493028	0.001707514200826437	0.0
18	2.276685601101916E-4	0.0	0.016050633487768508	0.001707514200826437	0.0
19	2.276685601101916E-4	0.0	0.01650597060798889	0.001707514200826437	0.0
20	2.276685601101916E-4	0.0	0.01684747344815418	0.0019351827609366285	0.0
21	2.276685601101916E-4	0.0	0.017188976288319464	0.0023905198811570117	0.0
22	2.276685601101916E-4	0.0	0.017416644848429655	0.0029596912814324906	0.0
23	2.276685601101916E-4	0.0	0.01787198196865004	0.0033011941215977778	0.0
24	2.276685601101916E-4	1.138342800550958E-4	0.01787198196865004	0.003870365521873257	0.0
25	2.276685601101916E-4	1.138342800550958E-4	0.018099650528760232	0.00432570264209364	0.0
26	2.276685601101916E-4	1.138342800550958E-4	0.018213484808815326	0.005236376882534407	0.0
27	2.276685601101916E-4	1.138342800550958E-4	0.018327319088870423	0.006260885403030269	0.0
28	2.276685601101916E-4	1.138342800550958E-4	0.018441153368925517	0.014570787847052262	0.0
29	2.276685601101916E-4	1.138342800550958E-4	0.018554987648980614	0.032215101255592106	0.0
30	2.276685601101916E-4	1.138342800550958E-4	0.018554987648980614	0.05361594590595012	0.0
31	2.276685601101916E-4	1.138342800550958E-4	0.018554987648980614	0.11815998269718943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	940	0.0	25.781916	1
CGCTCTC	165	0.0	23.545454	29
GTATCAA	2600	0.0	23.409615	1
CACGGTA	80	2.7239366E-8	23.125002	2
CCGCTCT	175	0.0	22.2	28
GCTTCGC	220	0.0	21.022728	22
ATAGGAC	115	1.3460522E-10	20.913044	3
GCCGCTC	190	0.0	20.447369	27
ACGGTAC	100	1.288754E-8	20.35	3
GGCCTAG	65	6.8997644E-5	19.923077	1
TTCGCCG	205	0.0	19.85366	24
GCCGGCA	215	0.0	19.790697	15
CCGGCAG	235	0.0	18.893618	16
AGCTTCG	235	0.0	18.893618	21
CGGCAGC	240	0.0	18.5	17
TTAACGG	140	9.458745E-11	18.5	35
GTATCAC	50	0.0070335935	18.5	1
CGAACTA	155	2.0008883E-11	17.903225	29
CTTCGCC	260	0.0	17.788462	23
TCACGGT	105	4.794074E-7	17.619047	1
>>END_MODULE