FastQCFastQC Report
Fri 10 Feb 2017
ERR1633339.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633339.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819217
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51460.6281607925616778No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.4368805823121346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19720.24071766088838487No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC13640.1665004510404447No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC11940.14574892855006671No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC11460.1398896751410188No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA11360.13866899734746715No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG11030.13464076062874672No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG10990.13415248951132605No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC10540.12865943944034364No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG9490.11584232260805134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.10949479808158279No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG8850.10802998472932081No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC8560.10449001912802103No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA8400.1025369346583384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCTC1950.027.51282127
GGTATCA10500.026.252381
CCGCTCT2050.026.1707328
TTCGCCG2200.024.38636424
CGGCAGC2300.024.13043617
CGCTCTC2250.023.84444429
GCCGGCA2450.023.40816315
CCGCCTA400.001930716423.12515
ACGTATA502.7011833E-422.229
CCGGCAG2500.022.19999916
GTATCAA26200.022.1011451
TGCTCGC2800.021.8035710
TCGCACC855.1761162E-821.76470628
GCTTCGC2550.021.76470622
GACGTAT603.724603E-521.58333428
CGCCGCT2500.021.4626
AGCTTCG2600.021.34615321
CGAGTCG1950.020.87179621
AGCGTCA909.4700226E-820.55555537
CCGTCCG1800.020.55555528
Produced by FastQC (version 0.11.5)