##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819217
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752645513948075	33.0	33.0	33.0	27.0	33.0
2	31.881610122836808	33.0	33.0	33.0	33.0	33.0
3	31.59471666237395	33.0	33.0	33.0	27.0	33.0
4	31.88640616588767	33.0	33.0	33.0	33.0	33.0
5	31.99309218436629	33.0	33.0	33.0	33.0	33.0
6	35.10419583577978	37.0	37.0	37.0	33.0	37.0
7	35.266676594846054	37.0	37.0	37.0	33.0	37.0
8	35.40778328574724	37.0	37.0	37.0	33.0	37.0
9	35.51899923951773	37.0	37.0	37.0	33.0	37.0
10	35.52399303237115	37.0	37.0	37.0	33.0	37.0
11	35.54694299556772	37.0	37.0	37.0	33.0	37.0
12	35.53259636946011	37.0	37.0	37.0	33.0	37.0
13	35.53216547019898	37.0	37.0	37.0	33.0	37.0
14	35.51478668045219	37.0	37.0	37.0	33.0	37.0
15	35.52560554773643	37.0	37.0	37.0	33.0	37.0
16	35.52202285841236	37.0	37.0	37.0	33.0	37.0
17	35.51867087719127	37.0	37.0	37.0	33.0	37.0
18	35.5048467011793	37.0	37.0	37.0	33.0	37.0
19	35.49124957123693	37.0	37.0	37.0	33.0	37.0
20	35.48055277173203	37.0	37.0	37.0	33.0	37.0
21	35.492384801584926	37.0	37.0	37.0	33.0	37.0
22	35.38820361393868	37.0	37.0	37.0	33.0	37.0
23	35.44608327219772	37.0	37.0	37.0	33.0	37.0
24	35.430968839757966	37.0	37.0	37.0	33.0	37.0
25	35.44276546995485	37.0	37.0	37.0	33.0	37.0
26	35.33471229234745	37.0	37.0	37.0	33.0	37.0
27	35.345404209141165	37.0	37.0	37.0	33.0	37.0
28	35.371687843391925	37.0	37.0	37.0	33.0	37.0
29	35.36767181345114	37.0	37.0	37.0	33.0	37.0
30	35.35941881088893	37.0	37.0	37.0	33.0	37.0
31	35.3563207306489	37.0	37.0	37.0	33.0	37.0
32	35.34126855277661	37.0	37.0	37.0	33.0	37.0
33	35.34691784960517	37.0	37.0	37.0	33.0	37.0
34	35.31809032283265	37.0	37.0	37.0	33.0	37.0
35	35.244653126094796	37.0	37.0	37.0	33.0	37.0
36	35.281927743198686	37.0	37.0	37.0	33.0	37.0
37	35.26927297651294	37.0	37.0	37.0	33.0	37.0
38	35.25448080301068	37.0	37.0	37.0	33.0	37.0
39	35.153000975321554	37.0	37.0	37.0	33.0	37.0
40	34.92755155227491	37.0	37.0	37.0	27.0	37.0
41	35.10236970180062	37.0	37.0	37.0	27.0	37.0
42	35.133849761418524	37.0	37.0	37.0	33.0	37.0
43	34.78265587750254	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	18.0
16	37.0
17	46.0
18	25.0
19	46.0
20	60.0
21	194.0
22	449.0
23	1022.0
24	2065.0
25	3749.0
26	6040.0
27	8947.0
28	12626.0
29	16766.0
30	21708.0
31	27635.0
32	35094.0
33	45780.0
34	65295.0
35	130034.0
36	441577.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.69823135994492	20.285589776579343	13.648520477480325	24.36765838599541
2	16.306302237380326	21.28862071954073	38.984542557100255	23.420534485978685
3	17.644165099112932	27.88723866814287	29.896596384108243	24.571999848635954
4	12.226186712433947	17.91356868814978	40.1142798550323	29.74596474438397
5	13.62166556602219	37.313190522169336	35.13501306735578	13.93013084445269
6	28.77271833958524	40.4886617343146	17.190561231029143	13.548058695071024
7	26.563901872153533	32.484677441996446	23.39770781123927	17.553712874610756
8	24.689429052375623	35.40429458861327	20.29901723230841	19.607259126702694
9	26.661311960078955	15.139578402303663	20.05817750364067	38.14093213397671
10	15.615886877347515	27.5759658307872	33.256389943079796	23.551757348785486
11	33.69180571203967	23.937857734885874	23.799921144214537	18.570415408859926
12	23.693355972837477	26.078194178099327	29.26831352376721	20.960136325295984
13	27.753818585307677	22.585712942968712	25.963450465505478	23.697018006218133
14	22.268702919983347	21.017630249372267	28.11025650102476	28.60341032961962
15	24.503275688859	28.08950497853438	25.29866933913725	22.108549993469374
16	22.601093483167464	28.302513253509144	25.22872450156674	23.86766876175665
17	22.395043071615945	27.100633897978195	26.15753823468019	24.346784795725675
18	23.178962350634812	26.623715084037563	28.01724085315612	22.1800817121715
19	25.395102884827832	25.633501257908463	27.629431518144763	21.341964339118938
20	24.33824005117081	25.768386154095925	27.996489330665746	21.89688446406752
21	23.271001456268607	26.755060014623723	27.838289488621452	22.13564904048622
22	22.922131742871546	26.663387112327992	27.58719606648788	22.827285078312585
23	22.716935805775513	26.555845398716095	28.373434633314492	22.3537841621939
24	23.096078328452656	27.175949717840325	26.860526575986583	22.867445377720433
25	22.855116532005564	26.56048397433159	28.374655311108043	22.209744182554804
26	23.104989276345584	26.99846316665792	28.023100106565174	21.87344745043133
27	23.2696587106957	26.666805010149936	27.093798102334304	22.969738176820062
28	22.147123411745607	26.35895007061621	28.685073674008233	22.808852843629953
29	24.17698851464264	26.147528676773064	27.34623427004078	22.32924853854351
30	23.55822694109131	25.928539080609898	28.081570572876295	22.431663405422494
31	23.024912813088598	26.396424878878243	28.09438768970859	22.48427461832457
32	21.887241109498458	26.069771501323824	28.317161387031764	23.725826002145954
33	21.983552587409687	26.512267201486296	28.570940300311147	22.933239910792867
34	23.07276338259582	26.178289757170564	28.349265213002173	22.399681647231443
35	23.02454660975053	26.949758122695204	27.62845497590992	22.397240291644337
36	23.912223501221288	26.58013688680777	27.09794840683238	22.409691205138564
37	22.935315063041905	26.136664644410455	27.59098016764789	23.33704012489975
38	23.363162629681756	26.523619504966327	27.345257727805944	22.767960137545973
39	22.35988755116166	26.60992142497043	27.81839244058656	23.211798583281354
40	22.72035370359746	27.106737286945947	27.996611398445097	22.17629761101149
41	22.577046130634496	25.942821010794454	28.03323173225165	23.4469011263194
42	22.06875589739959	27.336957118809792	27.60050145443759	22.993785529353026
43	23.179694757310944	25.755324901704917	27.74991241636831	23.315067924615825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	43.0
2	55.0
3	229.0
4	403.0
5	403.0
6	531.0
7	659.0
8	630.0
9	601.0
10	835.5
11	1070.0
12	1070.0
13	1808.5
14	2547.0
15	4519.5
16	6492.0
17	7224.5
18	7957.0
19	7957.0
20	6870.0
21	5783.0
22	5735.0
23	5687.0
24	7520.0
25	9353.0
26	9353.0
27	12066.5
28	14780.0
29	17692.0
30	20604.0
31	24856.5
32	29109.0
33	29109.0
34	36163.0
35	43217.0
36	47426.0
37	51635.0
38	55245.5
39	58856.0
40	58856.0
41	61650.0
42	64444.0
43	66099.0
44	67754.0
45	66322.0
46	64890.0
47	64890.0
48	62340.0
49	59790.0
50	60650.0
51	61510.0
52	58428.5
53	55347.0
54	55347.0
55	53730.0
56	52113.0
57	44452.5
58	36792.0
59	33318.0
60	29844.0
61	29844.0
62	26438.0
63	23032.0
64	18424.5
65	13817.0
66	11600.5
67	9384.0
68	9384.0
69	7928.5
70	6473.0
71	5601.0
72	4729.0
73	4768.0
74	4807.0
75	4807.0
76	4016.0
77	3225.0
78	2319.5
79	1414.0
80	916.5
81	419.0
82	419.0
83	339.5
84	260.0
85	212.5
86	165.0
87	137.5
88	110.0
89	110.0
90	77.5
91	45.0
92	28.0
93	11.0
94	6.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	819217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93234845049509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.06393356412354	42.11310386179953
2	7.708346381200329	7.543749822040146
3	1.9245093149326196	2.825122811835196
4	0.89780408429057	1.7572664917113536
5	0.5653847657324156	1.383280218271005
6	0.3838573236871917	1.1269824190761688
7	0.28930740570389785	0.9909543549648316
8	0.2248277245409805	0.8801078846856948
9	0.1920916569585394	0.8459546303455399
>10	1.3931725762713216	13.92573764950312
>50	0.19196769838976868	6.605592892918399
>100	0.15210124036545192	14.25414365408993
>500	0.010157011042739679	3.4129429732387218
>1k	0.0022853274846164275	1.6956615973419555
>5k	2.5392527606849197E-4	0.639398738178479
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5146	0.6281607925616778	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3579	0.4368805823121346	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1972	0.24071766088838487	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	1364	0.1665004510404447	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	1194	0.14574892855006671	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1146	0.1398896751410188	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	1136	0.13866899734746715	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	1103	0.13464076062874672	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG	1099	0.13415248951132605	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	1054	0.12865943944034364	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	949	0.11584232260805134	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	897	0.10949479808158279	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	885	0.10802998472932081	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	856	0.10449001912802103	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	840	0.1025369346583384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	3.662033380654942E-4	0.0	0.0
2	0.0	0.0	3.662033380654942E-4	0.0	0.0
3	0.0	0.0	3.662033380654942E-4	0.0	0.0
4	0.0	0.0	4.88271117420659E-4	2.441355587103295E-4	0.0
5	0.0	0.0	8.544744554861533E-4	2.441355587103295E-4	0.0
6	0.0	0.0	0.0018310166903274712	2.441355587103295E-4	0.0
7	0.0	0.0	0.003906168939365272	2.441355587103295E-4	0.0
8	0.0	0.0	0.00488271117420659	2.441355587103295E-4	0.0
9	0.0	0.0	0.006225456747113402	9.76542234841318E-4	0.0
10	2.441355587103295E-4	0.0	0.006835795643889226	0.0010986100141964828	0.0
11	2.441355587103295E-4	0.0	0.010986100141964827	0.0012206777935516474	0.0
12	2.441355587103295E-4	0.0	0.011718506818095815	0.0012206777935516474	0.0
13	2.441355587103295E-4	0.0	0.012572981273581968	0.0013427455729068123	0.0
14	2.441355587103295E-4	0.0	0.013671591287778452	0.0014648133522619769	0.0
15	2.441355587103295E-4	0.0	0.015258472419395593	0.0017089489109723066	0.0
16	2.441355587103295E-4	0.0	0.0169674213303679	0.0017089489109723066	1.2206777935516475E-4
17	4.88271117420659E-4	0.0	0.01769982800649889	0.001953084469682636	1.2206777935516475E-4
18	4.88271117420659E-4	0.0	0.018554302461985042	0.001953084469682636	3.662033380654942E-4
19	4.88271117420659E-4	0.0	0.0190425735794057	0.001953084469682636	3.662033380654942E-4
20	4.88271117420659E-4	0.0	0.01928670913811603	0.0020751522490378006	3.662033380654942E-4
21	6.103388967758237E-4	0.0	0.01977498025553669	0.0023192878077481303	3.662033380654942E-4
22	6.103388967758237E-4	0.0	0.020385319152312512	0.0031737622632342834	3.662033380654942E-4
23	6.103388967758237E-4	0.0	0.02050738693166768	0.004150304498075601	3.662033380654942E-4
24	6.103388967758237E-4	0.0	0.02050738693166768	0.0046385756154962605	3.662033380654942E-4
25	6.103388967758237E-4	0.0	0.02050738693166768	0.0046385756154962605	3.662033380654942E-4
26	6.103388967758237E-4	0.0	0.020629454711022843	0.005370982291627249	3.662033380654942E-4
27	6.103388967758237E-4	0.0	0.020751522490378007	0.007446134540665049	3.662033380654942E-4
28	7.324066761309884E-4	0.0	0.02087359026973317	0.017455692447788558	3.662033380654942E-4
29	7.324066761309884E-4	0.0	0.02087359026973317	0.03979409606978371	3.662033380654942E-4
30	7.324066761309884E-4	0.0	0.020995658049088338	0.07140965092277138	3.662033380654942E-4
31	7.324066761309884E-4	0.0	0.020995658049088338	0.14526065743264605	3.662033380654942E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCTC	195	0.0	27.512821	27
GGTATCA	1050	0.0	26.25238	1
CCGCTCT	205	0.0	26.17073	28
TTCGCCG	220	0.0	24.386364	24
CGGCAGC	230	0.0	24.130436	17
CGCTCTC	225	0.0	23.844444	29
GCCGGCA	245	0.0	23.408163	15
CCGCCTA	40	0.0019307164	23.125	15
ACGTATA	50	2.7011833E-4	22.2	29
CCGGCAG	250	0.0	22.199999	16
GTATCAA	2620	0.0	22.101145	1
TGCTCGC	280	0.0	21.80357	10
TCGCACC	85	5.1761162E-8	21.764706	28
GCTTCGC	255	0.0	21.764706	22
GACGTAT	60	3.724603E-5	21.583334	28
CGCCGCT	250	0.0	21.46	26
AGCTTCG	260	0.0	21.346153	21
CGAGTCG	195	0.0	20.871796	21
AGCGTCA	90	9.4700226E-8	20.555555	37
CCGTCCG	180	0.0	20.555555	28
>>END_MODULE