FastQCFastQC Report
Fri 10 Feb 2017
ERR1633338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888923
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36630.412071686749021No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25320.28483906930071556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15070.16953099424809573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCGG250.00549568129.632
GGTATCA8150.027.012271
TTCGCCG1000.025.90000224
GCCGGCA1150.025.7391315
GTCGATT901.4188117E-1024.66666612
GATAGAC601.3368372E-624.6666663
TCGATAC952.8012437E-1023.3684221
GTATCAA20850.022.448441
TCGATTG1005.347829E-1022.213
ACCGTTT603.7250644E-521.58333210
GGTCGAT1059.822543E-1021.14285911
CGATTGG1059.822543E-1021.14285914
TCTATAC1151.3460522E-1020.9130443
TAAACGA450.00382513720.55555529
TACACCG450.00382513720.5555555
GCTTCGC1450.020.41379422
GCAATAC555.141835E-420.1818183
CCGCTCT1457.2759576E-1219.1379328
CGCTCTC1501.2732926E-1118.49999829
TGGTCGA1258.571078E-917.7610
Produced by FastQC (version 0.11.5)