FastQCFastQC Report
Fri 10 Feb 2017
ERR1633337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918488
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91420.9953314577871458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63320.6893938734093423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38080.4145944203952583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.1681023595300102No Hit
GTATGAAACTGCTCGCCGGCAGCTTCGCCGCTCTCTTCCTGAG13660.14872268336657637No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA12490.1359843568996002No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT11220.12215728458074575No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11080.12063304038811612No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG10620.11562480946947591No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA9710.10571722221738335No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9250.10070899129874317No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG9210.10027349295799184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16150.028.523221
GTATCAA43900.023.1776771
CGAGACG502.7015296E-422.223
GCCGCTC3250.021.6307727
CGCTCTC3350.020.98507529
ACTGCGG450.003825232220.5555558
GCCGGCA3350.020.43283715
TCGGCGT555.142012E-420.18181811
TTCGCCG3600.020.04166624
CCGGCAG3350.019.88059616
GCTTCGC3450.019.84057822
CCGCTCT3550.019.80281828
CGAGTCG3200.019.07812521
AGCTTCG3700.018.521
CCGTCCG3200.018.528
TGCTCGC4000.018.037510
GGCGAGT3500.017.97142819
CGCCGTC3400.017.95588326
CTTCGCC4050.017.81481623
GCGAGTC3450.017.69565220
Produced by FastQC (version 0.11.5)