FastQCFastQC Report
Fri 10 Feb 2017
ERR1633336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900571
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54070.6003968593259166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.3949716346628972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20870.23174186155228182No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG16910.1877697594081977No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG15420.17122470077317611No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA15110.1677824402517958No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG12630.1402443560807532No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC9990.11092962131803044No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT9450.10493342557110988No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10204636835962962No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC9010.10004763644398942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA250.005495719629.5999984
GGTATCA11000.028.0863631
GGACCGT751.3729732E-824.6666646
GTATCAA28500.022.2649121
GCCGAGT957.1395334E-921.42105312
AGGACCG705.0999006E-621.1428575
GGCGCGT450.003825174920.55555532
CGAGTTC1153.0468073E-919.30434814
TGCGTCT500.00703372918.49999810
ACCATCG2250.018.08888818
GAGTTCC1552.0008883E-1117.90322715
CCGAGTT1156.400296E-817.69565213
CATTCCG953.604633E-617.52631636
GTAGCCC752.0666407E-417.2666663
CCGTACA1502.5102054E-1017.26666617
CCGTCCG1655.4569682E-1116.81818228
TCGGGAT2350.016.53191422
GCCGTCC1801.0913936E-1116.44444527
CCATCGG2400.016.187519
GTGTAAC803.3815487E-416.18751
Produced by FastQC (version 0.11.5)