##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633336.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	900571
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.920631466036546	33.0	33.0	33.0	33.0	33.0
2	32.01191022140397	33.0	33.0	33.0	33.0	33.0
3	31.735353459083182	33.0	33.0	33.0	27.0	33.0
4	32.00871225033895	33.0	33.0	33.0	33.0	33.0
5	32.108995292986336	33.0	33.0	33.0	33.0	33.0
6	35.274596894636844	37.0	37.0	37.0	33.0	37.0
7	35.430314766964514	37.0	37.0	37.0	33.0	37.0
8	35.56040334410058	37.0	37.0	37.0	33.0	37.0
9	35.67959105945006	37.0	37.0	37.0	33.0	37.0
10	35.67274207141913	37.0	37.0	37.0	33.0	37.0
11	35.69499684089317	37.0	37.0	37.0	33.0	37.0
12	35.68509756587765	37.0	37.0	37.0	33.0	37.0
13	35.6903386851231	37.0	37.0	37.0	33.0	37.0
14	35.674956222219016	37.0	37.0	37.0	33.0	37.0
15	35.685338524114144	37.0	37.0	37.0	33.0	37.0
16	35.683500801158374	37.0	37.0	37.0	33.0	37.0
17	35.66797065417385	37.0	37.0	37.0	33.0	37.0
18	35.64597349903561	37.0	37.0	37.0	33.0	37.0
19	35.65119129974205	37.0	37.0	37.0	33.0	37.0
20	35.65173317817251	37.0	37.0	37.0	33.0	37.0
21	35.643135299715404	37.0	37.0	37.0	33.0	37.0
22	35.5520486446932	37.0	37.0	37.0	33.0	37.0
23	35.60102201825286	37.0	37.0	37.0	33.0	37.0
24	35.59556436971655	37.0	37.0	37.0	33.0	37.0
25	35.61956136717705	37.0	37.0	37.0	33.0	37.0
26	35.5134597938419	37.0	37.0	37.0	33.0	37.0
27	35.52757972441928	37.0	37.0	37.0	33.0	37.0
28	35.54848534985026	37.0	37.0	37.0	33.0	37.0
29	35.55086939286297	37.0	37.0	37.0	33.0	37.0
30	35.55499677426877	37.0	37.0	37.0	33.0	37.0
31	35.54085907718547	37.0	37.0	37.0	33.0	37.0
32	35.535005013485886	37.0	37.0	37.0	33.0	37.0
33	35.5364640877843	37.0	37.0	37.0	33.0	37.0
34	35.51327657674964	37.0	37.0	37.0	33.0	37.0
35	35.45264615449531	37.0	37.0	37.0	33.0	37.0
36	35.48363760325393	37.0	37.0	37.0	33.0	37.0
37	35.47335745876783	37.0	37.0	37.0	33.0	37.0
38	35.464812879828465	37.0	37.0	37.0	33.0	37.0
39	35.36978650211921	37.0	37.0	37.0	33.0	37.0
40	35.15420994013798	37.0	37.0	37.0	33.0	37.0
41	35.32165370637074	37.0	37.0	37.0	33.0	37.0
42	35.35812168057821	37.0	37.0	37.0	33.0	37.0
43	35.03421162795604	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	34.0
16	41.0
17	49.0
18	33.0
19	30.0
20	44.0
21	120.0
22	364.0
23	823.0
24	1757.0
25	3125.0
26	5368.0
27	8178.0
28	11647.0
29	15710.0
30	21355.0
31	27448.0
32	35100.0
33	46705.0
34	68042.0
35	138806.0
36	515786.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.87990619284876	20.27924505674733	13.462236736470528	23.378612013933385
2	16.289443031143573	22.158719301420987	39.294625298838184	22.25721236859726
3	18.085303657346284	27.3714121374106	30.04393878994549	24.499345415297626
4	12.010157999757931	18.087968633233803	40.21926089114573	29.682612475862534
5	13.922500280377673	36.629427329993966	35.17568298335168	14.272389406276684
6	28.908992183847804	40.40580920327215	17.420281132748	13.264917480132048
7	27.18875024845348	32.202347177512934	23.074915803418055	17.53398677061553
8	24.639145608730463	35.45073070307616	20.555514223753597	19.354609464439783
9	26.272553746456417	15.17748184207575	20.335320591047235	38.214643820420605
10	15.202910153669173	28.03121575089582	33.800777506715185	22.965096588719824
11	33.893496459468494	24.32068099017179	23.68408487504039	18.101737675319328
12	23.208053557132086	25.75599258692541	30.45911982508875	20.576834030853757
13	28.349236206806573	22.653960653851833	26.306532189022295	22.690270950319295
14	22.432323492539734	21.383433399476555	28.06086360764448	28.12337950033923
15	23.69285708733681	28.84325611195564	25.398663736673733	22.065223064033816
16	23.106229270096417	28.6540428239417	25.13583048976705	23.103897416194837
17	22.82818345249847	27.165764831423616	26.051138666468276	23.954913049609637
18	23.1246620199851	26.282547406034617	28.4529481850959	22.139842388884386
19	25.7432229107977	26.055802374271437	27.628582310556304	20.572392404374558
20	25.0605449209446	26.171617784716585	27.604819608892576	21.163017685446235
21	23.068031282375294	27.102360613433035	27.546079098705157	22.283529005486518
22	23.377390566651602	26.19404799843655	27.851885081798105	22.576676353113747
23	22.50216806892516	26.542493595729823	28.560102423906606	22.39523591143841
24	22.690493031643257	27.346316947803118	27.28679915298183	22.676390867571797
25	22.90990937971576	27.111688029039353	28.25596205074336	21.722440540501527
26	23.7662549649056	26.758245601956983	27.19774454207386	22.27775489106356
27	23.14831368098684	26.627328661482547	26.99709406587598	23.22726359165463
28	22.644411156921553	26.508737234487896	28.2113237046274	22.63552790396315
29	23.740049368678317	26.9686676564091	27.46801751333321	21.823265461579375
30	23.692523965350873	26.43522831625713	27.57272885758036	22.29951886081164
31	23.051042061092353	26.01682710191645	27.90895998205583	23.02317085493537
32	21.950184938222527	26.619888937129886	28.061307770292405	23.36861835435518
33	22.39212677290297	26.278883064189273	28.460943112758464	22.868047050149297
34	23.14642598973318	26.339289184306402	28.164131423285895	22.350153402674525
35	23.03061057928803	27.08426098553029	27.17009541724084	22.71503301794084
36	23.1463149490712	26.507182665220174	27.212068787469285	23.13443359823934
37	23.25446855383973	26.31064069351556	27.877202352729547	22.557688399915165
38	22.865826236909694	26.252455386638037	27.24726867731695	23.634449699135327
39	22.645632604203332	26.246792312877055	28.059975282348642	23.04759980057097
40	23.11822166159026	26.898600998699713	27.787148375863758	22.19602896384627
41	23.014842805286868	26.254787240539613	27.696539195688068	23.03383075848545
42	22.056672933061357	27.895635102618225	27.471570814516568	22.576121149803846
43	23.607466818274183	25.830056708466074	27.311006017293472	23.25147045596627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	54.0
2	74.0
3	258.5
4	443.0
5	443.0
6	581.0
7	719.0
8	673.5
9	628.0
10	878.0
11	1128.0
12	1128.0
13	1844.5
14	2561.0
15	4547.0
16	6533.0
17	7642.5
18	8752.0
19	8752.0
20	8106.5
21	7461.0
22	8400.5
23	9340.0
24	11661.0
25	13982.0
26	13982.0
27	16902.5
28	19823.0
29	23310.5
30	26798.0
31	30709.0
32	34620.0
33	34620.0
34	42571.5
35	50523.0
36	53579.5
37	56636.0
38	58403.0
39	60170.0
40	60170.0
41	61308.5
42	62447.0
43	64280.5
44	66114.0
45	65088.0
46	64062.0
47	64062.0
48	62362.5
49	60663.0
50	62291.0
51	63919.0
52	64296.5
53	64674.0
54	64674.0
55	62307.0
56	59940.0
57	51337.0
58	42734.0
59	39259.5
60	35785.0
61	35785.0
62	31935.5
63	28086.0
64	21918.5
65	15751.0
66	13410.0
67	11069.0
68	11069.0
69	9522.0
70	7975.0
71	7880.5
72	7786.0
73	6960.5
74	6135.0
75	6135.0
76	3751.5
77	1368.0
78	1032.5
79	697.0
80	562.0
81	427.0
82	427.0
83	358.0
84	289.0
85	251.0
86	213.0
87	173.5
88	134.0
89	134.0
90	96.5
91	59.0
92	38.5
93	18.0
94	9.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	900571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.28801884183731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.40426590927387	49.19812966358166
2	7.7398412568343495	8.713206609950431
3	1.7158678494623842	2.897484055219246
4	0.7189105206383348	1.6186419572514261
5	0.4377965424611007	1.2321350015470829
6	0.28878635160504273	0.9753126960246057
7	0.2220561923908218	0.8749372198868859
8	0.17315978836660678	0.7797457144182498
9	0.12239996226937519	0.6200686244212888
>10	0.9238490742006911	10.534464726619754
>50	0.13585149153676357	5.3711330787455225
>100	0.10799815018833142	12.213041910447984
>500	0.007814337393959288	3.047846894440484
>1k	0.0012022057529168138	1.3140343460304202
>5k	2.0036762548613564E-4	0.6098175014150273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5407	0.6003968593259166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3557	0.3949716346628972	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2087	0.23174186155228182	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1691	0.1877697594081977	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1542	0.17122470077317611	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	1511	0.1677824402517958	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	1263	0.1402443560807532	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	999	0.11092962131803044	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	945	0.10493342557110988	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	919	0.10204636835962962	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	901	0.10004763644398942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	2.2208132396002092E-4	0.0	0.0
2	1.1104066198001046E-4	0.0	2.2208132396002092E-4	0.0	0.0
3	1.1104066198001046E-4	0.0	2.2208132396002092E-4	1.1104066198001046E-4	0.0
4	1.1104066198001046E-4	0.0	3.3312198594003136E-4	1.1104066198001046E-4	0.0
5	1.1104066198001046E-4	0.0	4.4416264792004184E-4	2.2208132396002092E-4	0.0
6	1.1104066198001046E-4	0.0	9.993659578200942E-4	2.2208132396002092E-4	0.0
7	1.1104066198001046E-4	0.0	0.0029980978734602823	3.3312198594003136E-4	0.0
8	2.2208132396002092E-4	0.0	0.0038864231693003662	3.3312198594003136E-4	0.0
9	2.2208132396002092E-4	0.0	0.00466370780316044	0.0011104066198001047	0.0
10	5.552033099000523E-4	0.0	0.005440992437020512	0.0011104066198001047	0.0
11	6.662439718800627E-4	0.0	0.007661805676620722	0.0012214472817801152	0.0
12	6.662439718800627E-4	0.0	0.009105334282360858	0.0012214472817801152	0.0
13	6.662439718800627E-4	0.0	0.009993659578200942	0.0013324879437601254	0.0
14	6.662439718800627E-4	0.0	0.010881984874041024	0.001443528605740136	0.0
15	6.662439718800627E-4	0.0	0.01210343215582114	0.001443528605740136	0.0
16	6.662439718800627E-4	0.0	0.013213838775621245	0.001443528605740136	0.0
17	6.662439718800627E-4	0.0	0.013213838775621245	0.001443528605740136	0.0
18	6.662439718800627E-4	0.0	0.013991123409481318	0.001443528605740136	0.0
19	6.662439718800627E-4	0.0	0.014213204733441339	0.0015545692677201464	0.0
20	6.662439718800627E-4	0.0	0.01443528605740136	0.0018876912536601779	0.0
21	6.662439718800627E-4	0.0	0.014657367381361381	0.0022208132396002093	0.0
22	6.662439718800627E-4	0.0	0.014990489367301412	0.0029980978734602823	0.0
23	6.662439718800627E-4	0.0	0.015323611353241444	0.0036643418453403452	0.0
24	6.662439718800627E-4	0.0	0.015434652015221454	0.004219545155240398	0.0
25	6.662439718800627E-4	0.0	0.015434652015221454	0.004552667141180429	0.0
26	6.662439718800627E-4	0.0	0.015434652015221454	0.005329951775040502	0.0
27	6.662439718800627E-4	0.0	0.015434652015221454	0.0066624397188006276	0.0
28	6.662439718800627E-4	0.0	0.015434652015221454	0.01643401797304155	0.0
29	6.662439718800627E-4	0.0	0.015434652015221454	0.03631029646746342	0.0
30	6.662439718800627E-4	0.0	0.015434652015221454	0.05985091680722564	0.0
31	6.662439718800627E-4	0.0	0.015434652015221454	0.12858508657285211	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	25	0.0054957196	29.599998	4
GGTATCA	1100	0.0	28.086363	1
GGACCGT	75	1.3729732E-8	24.666664	6
GTATCAA	2850	0.0	22.264912	1
GCCGAGT	95	7.1395334E-9	21.421053	12
AGGACCG	70	5.0999006E-6	21.142857	5
GGCGCGT	45	0.0038251749	20.555555	32
CGAGTTC	115	3.0468073E-9	19.304348	14
TGCGTCT	50	0.007033729	18.499998	10
ACCATCG	225	0.0	18.088888	18
GAGTTCC	155	2.0008883E-11	17.903227	15
CCGAGTT	115	6.400296E-8	17.695652	13
CATTCCG	95	3.604633E-6	17.526316	36
GTAGCCC	75	2.0666407E-4	17.266666	3
CCGTACA	150	2.5102054E-10	17.266666	17
CCGTCCG	165	5.4569682E-11	16.818182	28
TCGGGAT	235	0.0	16.531914	22
GCCGTCC	180	1.0913936E-11	16.444445	27
CCATCGG	240	0.0	16.1875	19
GTGTAAC	80	3.3815487E-4	16.1875	1
>>END_MODULE