FastQCFastQC Report
Fri 10 Feb 2017
ERR1633335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577860
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27890.4826428546706815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20090.3476620634755823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11460.19831793167895337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.028.1199991
AACTCGC601.3354856E-624.66666622
ACGGGTA701.9194886E-723.78571316
GTATCAA14200.023.7112671
TAGTGCA806.947794E-720.81259
GTACTAT555.138842E-420.1818181
CCTTAGA759.252699E-619.7333341
CCGGGCT759.252699E-619.73333428
ATAGTGC851.2431155E-619.5882368
AGCGTGA701.2178664E-418.516
CGGCGTC500.007030752518.524
CCGGCGT500.007030752518.523
ACGGGTT609.229242E-418.516
TCTAGAC609.229242E-418.53
GCGTCAA500.007030752518.526
ATCGCTA500.007030752518.510
CGAACTA1054.787071E-717.6190529
CCTAGAG752.0650252E-417.2666662
TTAGACC650.001578648817.0769233
TTTCCCG650.001578648817.07692333
Produced by FastQC (version 0.11.5)