FastQCFastQC Report
Fri 10 Feb 2017
ERR1633334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725779
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38660.5326690356155249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26030.3586491204622895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16140.2223817443050846No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC8300.11435988090038426No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7620.10499063764589497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7900.026.2278481
GCTACCG400.00193050523.1257
GTATCAA21150.022.5673771
CCGATCG502.7007726E-422.29
AACGGCC1950.019.92307737
CGCTCTC1600.019.6562529
TTCGCCG1700.019.58823424
CCGCTCT1650.019.06060828
TTAACGG1950.018.9743635
GGACCGT801.6153328E-518.56
CCGGCAG2050.018.04878216
TTTAACG2400.017.72916834
GCCGGCA2100.017.61904715
GACCGTT650.001579175517.0769237
TGCGGTA650.001579175517.07692336
TTGCGGT650.001579175517.07692335
GCCGCTC1757.2759576E-1216.91428627
AGCTTCG2200.016.81818221
TAACGGC2200.016.81818236
GCTTCGC2100.016.73809422
Produced by FastQC (version 0.11.5)