##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1633334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725779
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.778748076205016	33.0	33.0	33.0	27.0	33.0
2	31.908341244373286	33.0	33.0	33.0	33.0	33.0
3	31.62384141729094	33.0	33.0	33.0	27.0	33.0
4	31.91606811439846	33.0	33.0	33.0	33.0	33.0
5	32.014965988269154	33.0	33.0	33.0	33.0	33.0
6	35.17218602356916	37.0	37.0	37.0	33.0	37.0
7	35.335143342532646	37.0	37.0	37.0	33.0	37.0
8	35.45568967964077	37.0	37.0	37.0	33.0	37.0
9	35.58475238330125	37.0	37.0	37.0	33.0	37.0
10	35.5955297687037	37.0	37.0	37.0	33.0	37.0
11	35.602146107837235	37.0	37.0	37.0	33.0	37.0
12	35.59921133017075	37.0	37.0	37.0	33.0	37.0
13	35.598379120916974	37.0	37.0	37.0	33.0	37.0
14	35.59141143516139	37.0	37.0	37.0	33.0	37.0
15	35.592717617897456	37.0	37.0	37.0	33.0	37.0
16	35.57635864360914	37.0	37.0	37.0	33.0	37.0
17	35.57952214103742	37.0	37.0	37.0	33.0	37.0
18	35.56241087162897	37.0	37.0	37.0	33.0	37.0
19	35.54326454747244	37.0	37.0	37.0	33.0	37.0
20	35.556468291311816	37.0	37.0	37.0	33.0	37.0
21	35.55366440748492	37.0	37.0	37.0	33.0	37.0
22	35.45438487473459	37.0	37.0	37.0	33.0	37.0
23	35.50674103273862	37.0	37.0	37.0	33.0	37.0
24	35.499748546045005	37.0	37.0	37.0	33.0	37.0
25	35.51900647442265	37.0	37.0	37.0	33.0	37.0
26	35.40932019251039	37.0	37.0	37.0	33.0	37.0
27	35.42100556781059	37.0	37.0	37.0	33.0	37.0
28	35.44530635358697	37.0	37.0	37.0	33.0	37.0
29	35.44484753623348	37.0	37.0	37.0	33.0	37.0
30	35.452360842625644	37.0	37.0	37.0	33.0	37.0
31	35.43192762535152	37.0	37.0	37.0	33.0	37.0
32	35.42975892110408	37.0	37.0	37.0	33.0	37.0
33	35.42474086464337	37.0	37.0	37.0	33.0	37.0
34	35.403633888552854	37.0	37.0	37.0	33.0	37.0
35	35.34571543128142	37.0	37.0	37.0	33.0	37.0
36	35.37505080747721	37.0	37.0	37.0	33.0	37.0
37	35.3715332077671	37.0	37.0	37.0	33.0	37.0
38	35.3545142529613	37.0	37.0	37.0	33.0	37.0
39	35.25187694876815	37.0	37.0	37.0	33.0	37.0
40	35.03625070441553	37.0	37.0	37.0	27.0	37.0
41	35.21224091631199	37.0	37.0	37.0	33.0	37.0
42	35.23773903626311	37.0	37.0	37.0	33.0	37.0
43	34.921425117012205	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	4.0
15	15.0
16	31.0
17	29.0
18	18.0
19	19.0
20	54.0
21	112.0
22	330.0
23	791.0
24	1669.0
25	2979.0
26	4941.0
27	7432.0
28	10391.0
29	14156.0
30	18389.0
31	23489.0
32	29994.0
33	39223.0
34	56629.0
35	113967.0
36	401117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.299486482799864	20.46022273998008	13.814949178744493	24.42534159847557
2	15.875493779786959	21.662930451280623	39.009533205011444	23.452042563920976
3	17.76973431306224	27.32911809242207	30.21484501480478	24.686302579710905
4	12.328684076006608	18.135134799987323	39.67805626781706	29.858124856189004
5	13.877502655767113	36.93011233447096	35.34643465848419	13.84595035127773
6	29.07262403569131	40.23662850537147	17.374710483494287	13.316036975442938
7	26.338320618259832	32.47076589430116	23.532645612507388	17.658267874931624
8	24.506909127985242	35.70067472329731	20.59208106048811	19.200335088229338
9	26.08769336120224	15.418192039174459	20.27283787489029	38.22127672473301
10	15.03763542345535	28.059367934316093	33.66934011593061	23.23365652629795
11	33.683807329779455	23.713141328145344	24.235476639583123	18.36757470249208
12	22.983718184185545	25.8365149721885	30.372744320240734	20.807022523385218
13	28.055923359590178	22.553835258391327	25.990280787953356	23.399960594065135
14	22.015517120225304	21.119238776542172	28.077279722890854	28.787964380341673
15	24.02935328798436	28.637367573324664	24.67583107254412	22.657448066146856
16	22.620384442096007	28.641638845984797	25.381417759400588	23.356558952518604
17	22.283918382868613	27.469105609283268	26.493188697936976	23.753787309911143
18	23.13775956592847	27.090753521388745	27.904362071649906	21.867124841032876
19	24.74816714178834	26.34906769140468	27.667650896485018	21.235114270321958
20	24.26441106728081	26.12599703215442	27.712430367921915	21.897161532642855
21	22.956574935345333	26.923484972698304	27.827754729745557	22.292185362210812
22	22.67935556140368	26.803889338214525	27.713808197812284	22.802946902569516
23	22.672053062984737	26.689805023292212	28.16849206163309	22.46964985208996
24	22.939627627693827	27.20649123217949	26.920315963950458	22.933565176176216
25	22.948859087959285	26.770683637856703	28.045038503456286	22.235418770727726
26	22.979171345547336	27.161987326720666	27.71601272563687	22.142828602095125
27	22.90545744641275	26.90584875010161	27.299081400812092	22.88961240267354
28	22.42859052135705	26.731966617937413	27.943630223525346	22.89581263718019
29	23.714381375046674	26.691320636171618	27.181276945185793	22.413021043595915
30	23.33437589128371	26.331018119840888	27.873912031072816	22.460693957802583
31	23.007279075310805	26.5726894826111	27.726208666825574	22.693822775252524
32	22.018272780006036	26.642821024030734	28.09395146456428	23.244954731398952
33	22.058918761771835	26.691320636171618	28.419808233635855	22.829952368420688
34	22.888234572783176	26.441244511070174	28.064328121921413	22.606192794225237
35	22.715316921542232	27.06925937509903	27.602892891637815	22.612530811720923
36	23.53085443364991	26.75855873482148	27.423912788879257	22.286674042649345
37	22.87597188675892	26.456400639864203	27.60220397669263	23.065423496684254
38	23.041862605558993	26.790524388277976	27.33655837383005	22.831054632332982
39	22.62603354464651	26.52088307873333	27.793722331453512	23.05936104516664
40	22.707187725189073	27.257470938123035	27.812874166929603	22.222467169758286
41	22.779661577422328	26.36573943307811	27.647396797096636	23.20720219240292
42	22.23693438360713	27.265875700454274	27.674540045936848	22.822649870001747
43	22.934116308132367	26.386269098444572	27.46083862994107	23.218775963481995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	34.0
2	47.0
3	175.0
4	303.0
5	303.0
6	382.0
7	461.0
8	466.5
9	472.0
10	654.0
11	836.0
12	836.0
13	1332.0
14	1828.0
15	3318.0
16	4808.0
17	5596.5
18	6385.0
19	6385.0
20	5876.0
21	5367.0
22	5690.0
23	6013.0
24	7881.0
25	9749.0
26	9749.0
27	12370.0
28	14991.0
29	17942.0
30	20893.0
31	24681.5
32	28470.0
33	28470.0
34	33467.0
35	38464.0
36	41805.0
37	45146.0
38	48220.5
39	51295.0
40	51295.0
41	53378.5
42	55462.0
43	57022.5
44	58583.0
45	57463.5
46	56344.0
47	56344.0
48	54866.5
49	53389.0
50	53641.5
51	53894.0
52	51155.0
53	48416.0
54	48416.0
55	45999.5
56	43583.0
57	38285.5
58	32988.0
59	30568.5
60	28149.0
61	28149.0
62	24504.5
63	20860.0
64	17242.0
65	13624.0
66	11508.5
67	9393.0
68	9393.0
69	7831.5
70	6270.0
71	5121.0
72	3972.0
73	3161.0
74	2350.0
75	2350.0
76	1790.0
77	1230.0
78	957.5
79	685.0
80	562.0
81	439.0
82	439.0
83	351.5
84	264.0
85	227.5
86	191.0
87	140.0
88	89.0
89	89.0
90	67.5
91	46.0
92	27.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	725779.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.61711793709005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.82562971260002	53.49945065963385
2	7.713581548312051	9.505773279594106
3	2.024680661550096	3.7426496132303324
4	0.9293960195314215	2.2906681658291856
5	0.5087821664837628	1.5674845378259101
6	0.3397073394900675	1.2559072320872746
7	0.2480923248844979	1.0700713829186475
8	0.18778828249126633	0.9256778199574353
9	0.14611221958549886	0.8102712479624713
>10	0.9051442400443634	10.744371451736825
>50	0.099347433434786	4.317398091750261
>100	0.06811720356253122	8.012581011635806
>500	0.0029419390241742353	1.13059077026333
>1k	6.789090055786697E-4	1.1271047355745556
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3866	0.5326690356155249	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2603	0.3586491204622895	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1614	0.2223817443050846	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	830	0.11435988090038426	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	762	0.10499063764589497	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	8.266979342196454E-4	0.0	0.0
2	1.3778298903660755E-4	0.0	8.266979342196454E-4	1.3778298903660755E-4	0.0
3	1.3778298903660755E-4	0.0	9.644809232562529E-4	1.3778298903660755E-4	0.0
4	1.3778298903660755E-4	0.0	9.644809232562529E-4	1.3778298903660755E-4	0.0
5	1.3778298903660755E-4	0.0	9.644809232562529E-4	1.3778298903660755E-4	0.0
6	1.3778298903660755E-4	0.0	0.0019289618465125058	2.755659780732151E-4	0.0
7	1.3778298903660755E-4	0.0	0.0048224046162812645	2.755659780732151E-4	0.0
8	2.755659780732151E-4	0.0	0.005511319561464303	2.755659780732151E-4	0.0
9	4.133489671098227E-4	0.0	0.00675136646279377	8.266979342196454E-4	0.0
10	8.266979342196454E-4	0.0	0.0073024984189402	8.266979342196454E-4	0.0
11	8.266979342196454E-4	0.0	0.010058158199672353	9.644809232562529E-4	0.0
12	8.266979342196454E-4	0.0	0.010609290155818782	0.0012400469013294682	0.0
13	8.266979342196454E-4	0.0	0.011573771079075035	0.0012400469013294682	0.0
14	8.266979342196454E-4	0.0	0.012262686024258073	0.0013778298903660757	0.0
15	8.266979342196454E-4	0.0	0.013227166947514326	0.0015156128794026832	0.0
16	8.266979342196454E-4	0.0	0.014329430859807186	0.0015156128794026832	0.0
17	8.266979342196454E-4	0.0	0.014467213848843793	0.0015156128794026832	0.0
18	8.266979342196454E-4	0.0	0.015156128794026832	0.0015156128794026832	0.0
19	8.266979342196454E-4	0.0	0.015431694772100046	0.0016533958684392907	0.0
20	8.266979342196454E-4	0.0	0.01653395868439291	0.0016533958684392907	0.0
21	8.266979342196454E-4	0.0	0.01749843960764916	0.0023423108136223286	0.0
22	8.266979342196454E-4	0.0	0.01791178857475898	0.0024800938026589363	0.0
23	8.266979342196454E-4	0.0	0.01804957156379559	0.0031690087478419737	0.0
24	8.266979342196454E-4	0.0	0.0181873545528322	0.0033067917368785815	0.0
25	8.266979342196454E-4	0.0	0.018325137541868805	0.0037201407039884043	0.0
26	8.266979342196454E-4	0.0	0.018325137541868805	0.003995706682061619	0.0
27	8.266979342196454E-4	0.0	0.018325137541868805	0.00454683863820805	0.0
28	8.266979342196454E-4	0.0	0.018325137541868805	0.011987120046184858	0.0
29	9.644809232562529E-4	0.0	0.018462920530905414	0.03113895552227331	0.0
30	0.0011022639122928604	0.0	0.018462920530905414	0.05897111930766804	0.0
31	0.0011022639122928604	0.0	0.018462920530905414	0.1202845494289584	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	790	0.0	26.227848	1
GCTACCG	40	0.001930505	23.125	7
GTATCAA	2115	0.0	22.567377	1
CCGATCG	50	2.7007726E-4	22.2	9
AACGGCC	195	0.0	19.923077	37
CGCTCTC	160	0.0	19.65625	29
TTCGCCG	170	0.0	19.588234	24
CCGCTCT	165	0.0	19.060608	28
TTAACGG	195	0.0	18.97436	35
GGACCGT	80	1.6153328E-5	18.5	6
CCGGCAG	205	0.0	18.048782	16
TTTAACG	240	0.0	17.729168	34
GCCGGCA	210	0.0	17.619047	15
GACCGTT	65	0.0015791755	17.076923	7
TGCGGTA	65	0.0015791755	17.076923	36
TTGCGGT	65	0.0015791755	17.076923	35
GCCGCTC	175	7.2759576E-12	16.914286	27
AGCTTCG	220	0.0	16.818182	21
TAACGGC	220	0.0	16.818182	36
GCTTCGC	210	0.0	16.738094	22
>>END_MODULE