FastQCFastQC Report
Fri 10 Feb 2017
ERR1633333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1633333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748839
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31900.4259927701415124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.3024682208058074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13080.17467038976335367No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9440.12606181034908706No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG9280.12392516949571271No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG8540.1140432055488563No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA7870.10509602197535117No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC7840.10469540181534348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTCG1250.025.16000221
GCCGGCA1350.023.29629715
GCCGCTC1300.022.7692327
GGTATCA7150.021.9930081
TTCGCCG1350.021.92592624
GCTTCGC1450.021.68965522
GTATCAA16800.021.4732131
TGCTCGC1650.021.3030310
CCGCTCT1400.021.14285928
CGCTCTC1500.020.96666529
AGGACCG1001.2880264E-820.355
TAGGACC2000.020.354
CGCCGCT1650.020.18181826
CCGTTTA555.140794E-420.18181827
GCTCGCC1750.020.08571411
TCGCCGC1700.019.58823425
CGTTAGA851.2441233E-619.5882342
AACGATT1052.2562745E-819.3809522
TTAACGG2100.019.3809535
CTTCGCC1650.019.06060623
Produced by FastQC (version 0.11.5)